Anti-Cathepsin D/Ctsd Antibody Picoband™

Boster Bio Anti-Cathepsin D/Ctsd Antibody Picoband™ catalog # A01361. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A01361
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC, WB

Product Name

Anti-Cathepsin D/Ctsd Antibody Picoband™

See all Cathepsin D products

SKU/Catalog Number

A01361

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cathepsin D/Ctsd Antibody Picoband™ catalog # A01361. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cathepsin D/Ctsd Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01361)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse Cathepsin D recombinant protein (Position: K161-Q358).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01361 is reactive to Ctsd in Mouse, Rat

Applications

A01361 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
Direct ELISA,0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Ctsd (Source: Uniprot.Org, NCBI)

Uniprot ID

P18242

Gene ID

13033

Gene Name

Ctsd

Full Name

Cathepsin D

Weight

44.954kDa

Superfamily

peptidase A1 family

Alternative Names

cathepsin D (lysosomal aspartyl protease); Cathepsin D; CPSD; CTSD; EC 3.4.23; EC 3.4.23.5; lysosomal aspartyl peptidase; lysosomal aspartyl protease; MGC2311; neuronal 10 Ctsd|CD, Cat, CatD|cathepsin D|cathepsin D

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ctsd, check out the Ctsd Infographic

Ctsd infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ctsd: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01361 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ma WQ,Sun XJ,Zhu Y,Liu NF. PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming. Cell Death Dis.2020 Nov 17;11(11):991.doi:10.1038/s41419-020-03162-w. PMID:33203874;PMCID:PMC7673024.
Species: Rat
A01361 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA

Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage checkpoint

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16 Customer Q&As for Anti-Cathepsin D/Ctsd Antibody Picoband™

Question

See below the WB image, lot number and protocol we used for adult mammalian kidney using anti-Cathepsin D/Ctsd antibody A01361. Please let me know if you require anything else.

C. Patel

Verified customer

Asked: 2020-02-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

We were well pleased with the WB result of your anti-Cathepsin D/Ctsd antibody. However we have observed positive staining in platelet lysosome. melanosome. secreted, using this antibody. Is that expected? Could you tell me where is CTSD supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

From what I have seen in literature, platelet does express CTSD. Generally CTSD expresses in lysosome. melanosome. secreted,. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789

Boster Scientific Support

Answered: 2020-02-24

Question

Is this A01361 anti-Cathepsin D/Ctsd antibody reactive to the isotypes of CTSD?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

The immunogen of A01361 anti-Cathepsin D/Ctsd antibody is E. coli-derived mouse Cathepsin D recombinant protein (Position: K161-Q358). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-24

Question

Does anti-Cathepsin D/Ctsd antibody A01361 work for WB with adult mammalian kidney?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

According to the expression profile of adult mammalian kidney, CTSD is highly expressed in adult mammalian kidney. So, it is likely that anti-Cathepsin D/Ctsd antibody A01361 will work for WB with adult mammalian kidney.

Boster Scientific Support

Answered: 2019-10-30

Question

We have observed staining in rat liver. What should we do? Is anti-Cathepsin D/Ctsd antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

Based on literature liver does express CTSD. Based on Uniprot.org, CTSD is expressed in adult mammalian kidney, kidney, liver, melanoma, plasma, platelet, spleen, among other tissues. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789

Boster Scientific Support

Answered: 2019-10-28

Question

We want using your anti-Cathepsin D/Ctsd antibody for r-mmu-2132295; mhc class ii antigen presentation studies. Has this antibody been tested with western blotting on mouse testis tissue? We would like to see some validation images before ordering.

D. Anderson

Verified customer

Asked: 2019-09-19

Answer

Thank you for your inquiry. This A01361 anti-Cathepsin D/Ctsd antibody is tested on mouse testis tissue, rat testis tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-19

Question

I see that the anti-Cathepsin D/Ctsd antibody A01361 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-03

Question

Can you help my question with product A01361, anti-Cathepsin D/Ctsd antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Li

Verified customer

Asked: 2019-08-12

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01361 anti-Cathepsin D/Ctsd antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-12

Question

Do you have a BSA free version of anti-Cathepsin D/Ctsd antibody A01361 available?

Z. Singh

Verified customer

Asked: 2019-07-01

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin D/Ctsd antibody A01361 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-01

Question

Will A01361 anti-Cathepsin D/Ctsd antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

It shows on the product datasheet, A01361 anti-Cathepsin D/Ctsd antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-24

Question

Is a blocking peptide available for product anti-Cathepsin D/Ctsd antibody (A01361)?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

We do provide the blocking peptide for product anti-Cathepsin D/Ctsd antibody (A01361). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-13

Question

We ordered your anti-Cathepsin D/Ctsd antibody for ELISA on spleen last year. I am using mouse, and I plan to use the antibody for WB next. I would like examining spleen as well as melanoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?

O. Krishna

Verified customer

Asked: 2018-08-17

Answer

I have checked the website and datasheets of our anti-Cathepsin D/Ctsd antibody and it appears that A01361 has been validated on mouse in both ELISA and WB. Thus A01361 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-08-17

Question

We are currently using anti-Cathepsin D/Ctsd antibody A01361 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-13

Answer

The anti-Cathepsin D/Ctsd antibody (A01361) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-13

Question

I am interested in to test anti-Cathepsin D/Ctsd antibody A01361 on mouse adult mammalian kidney for research purposes, then I may be interested in using anti-Cathepsin D/Ctsd antibody A01361 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-02-22

Answer

The products we sell, including anti-Cathepsin D/Ctsd antibody A01361, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-02-22

Question

I was wanting to use your anti-Cathepsin D/Ctsd antibody for WB for mouse adult mammalian kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse adult mammalian kidney identification?

Verified Customer

Verified customer

Asked: 2017-06-08

Answer

It shows on the product datasheet, A01361 anti-Cathepsin D/Ctsd antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse adult mammalian kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-08

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adult mammalian kidney using anti-Cathepsin D/Ctsd antibody A01361. Let me know if you need anything else.

R. Mitchell

Verified customer

Asked: 2016-11-04

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-11-04

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A01361
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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