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Product Info Summary
|Reactive Species:||Mouse, Rat|
|Application:||ELISA, IF, ICC, WB|
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Anti-Cathepsin D/Ctsd Antibody Picoband™
Boster Bio Anti-Cathepsin D/Ctsd Antibody Picoband™ catalog # A01361. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin D/Ctsd Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01361)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived mouse Cathepsin D recombinant protein (Position: K161-Q358).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01361 is reactive to Ctsd in Mouse, Rat
A01361 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Background of Cathepsin D
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunocytochemistry/Immunofluorescence, 5 μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cathepsin D using anti-Cathepsin D antibody (A01361).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates,
Lane 2: rat testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin D antigen affinity purified polyclonal antibody (Catalog # A01361) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 45KD. The expected band size for Cathepsin D is at 45KD.
Gene/Protein Information For Ctsd (Source: Uniprot.Org, NCBI)
peptidase A1 family
cathepsin D (lysosomal aspartyl protease); Cathepsin D; CPSD; CTSD; EC 3.4.23; EC 22.214.171.124; lysosomal aspartyl peptidase; lysosomal aspartyl protease; MGC2311; neuronal 10 Ctsd|CD, Cat, CatD|cathepsin D|cathepsin D*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ctsd, check out the Ctsd Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ctsd: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A01361 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma WQ,Sun XJ,Zhu Y,Liu NF. PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming. Cell Death Dis.2020 Nov 17;11(11):991.doi:10.1038/s41419-020-03162-w. PMID:33203874;PMCID:PMC7673024.
A01361 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA
Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage checkpoint
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