Anti-CD11c/Itgax Antibody Picoband®

Western blot analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (A00357-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11c/Itgax antigen affinity purified polyclonal antibody (Catalog # A00357-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11c/Itgax at approximately 127 kDa. The expected band size for CD11c/Itgax is at 127 kDa.

Effects of 1,25 VD3 supplementation on macrophage polarization and PPARγ expression in NASH rats. (A) M1-type KCs identified by double immunofluorescence staining; green arrows indicate CD68-positive cells, red arrows indicate CD11c-positive cells, and yellow arrows indicate CD11c/CD68 double-positive cells (M1KCs). (B) M2-type KCs identified by double immunofluorescence staining; green arrows indicate CD68-positive cells, red arrows indicate CD163-positive cells, and yellow arrows indicate CD163/CD68 double-positive cells (M2KCs). Antibodies were diluted as follows: anti-CD68 (1:100), anti-CD11c (1:100), and anti-CD163 (1:200). (C) The M1/M2 macrophage ratio was determined by quantifying the proportion of M1 and M2 KCs in liver tissue, as identified through double immunofluorescence staining. (D) PPARγ levels in liver tissue of the indicated groups were determined by qPCR and expressed as relative mRNA levels following normalization to GAPDH mRNA levels. Data are presented as mean ± SEM, n=6. *p < 0.05 and ***p < 0.001 vs CG; ##p < 0.01 and ###p < 0.001 vs CDG.
Index in PubMed under a CC BY license. PMID: 40190400

Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating CD163-positive cells, green arrows showing CD68-positive cells, and yellow arrows marking CD163 and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.
Index in PubMed under a CC BY license. PMID: 40626231

IHC analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (A00357-3).
CD11c/Itgax was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD11c/Itgax Antibody (A00357-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (A00357-3).
CD11c/Itgax was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD11c/Itgax Antibody (A00357-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (A00357-3).
CD11c/Itgax was detected in a paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD11c/Itgax Antibody (A00357-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.