Anti-CD11c/ITGAX Antibody Picoband™

CD11c antibody

Boster Bio Anti-CD11c/ITGAX Antibody Picoband™ catalog # A00357-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. Cited in 2 publication(s).

Product Info Summary

SKU: A00357-1
Size: 100μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-CD11c/ITGAX Antibody Picoband™

View all CD11c Antibodies

SKU/Catalog Number

A00357-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD11c/ITGAX Antibody Picoband™ catalog # A00357-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD11c/ITGAX Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00357-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E. coli-derived human CD11c recombinant protein (Position: S161-T342).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00357-1 is reactive to ITGAX in Human, Mouse

Applications

A00357-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of CD11c

CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a genethat encodes for CD11c. This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ITGAX (Source: Uniprot.Org, NCBI)

Uniprot ID

P20702

Gene ID

3687

Gene Name

ITGAX

Full Name

Integrin alpha-X

Weight

127.829kDa

Superfamily

integrin alpha chain family

Alternative Names

95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6 ITGAX CD11C, SLEB6 integrin subunit alpha X integrin alpha-X|CD11 antigen-like family member C|complement component 3 receptor 4 subunit|integrin alpha X|integrin, alpha X (antigen CD11C (p150), alpha polypeptide)|integrin, alpha X (complement component 3 receptor 4 subunit)|leu M5, alpha subunit|leukocyte adhesion glycoprotein p150,95 alpha chain|leukocyte adhesion receptor p150,95|leukocyte surface antigen p150,95, alpha subunit|myeloid membrane antigen, alpha subunit|p150 95 integrin alpha chain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ITGAX, check out the ITGAX Infographic

ITGAX infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ITGAX: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00357-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Mu L, Jing C, Guo Z. Iran J Basic Med Sci. 2014 Nov;17(11):874-8. Expressions Of Cd11A, Cd11B, And Cd11C Integrin Proteins In Rats With Myocardial Hypertrophy.

Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

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Find answers in Q&As, reviews.

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17 Customer Q&As for Anti-CD11c/ITGAX Antibody Picoband™

Question

Will A00357-1 anti-CD11c/ITGAX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

As indicated on the product datasheet, A00357-1 anti-CD11c/ITGAX antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-22

Question

I was wanting to use your anti-CD11c/ITGAX antibody for ELISA for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

It shows on the product datasheet, A00357-1 anti-CD11c/ITGAX antibody has been validated for ELISA, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-19

Question

Would anti-CD11c/ITGAX antibody A00357-1 work on horse WB with blood?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

Our lab technicians have not validated anti-CD11c/ITGAX antibody A00357-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CD11c/ITGAX antibody A00357-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-20

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD11c/ITGAX antibody A00357-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-13

Question

I see that the anti-CD11c/ITGAX antibody A00357-1 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-11

Question

We have observed staining in mouse liver. Any tips? Is anti-CD11c/ITGAX antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

From literature liver does express ITGAX. From Uniprot.org, ITGAX is expressed in blood, liver, among other tissues. Regarding which tissues have ITGAX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2019-07-29

Question

My question regarding product A00357-1, anti-CD11c/ITGAX antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00357-1 anti-CD11c/ITGAX antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-24

Question

We bought anti-CD11c/ITGAX antibody for IHC on liver a few months ago. I am using human, and We want to use the antibody for ELISA next. My lab would like examining liver as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-02-26

Answer

I looked at the website and datasheets of our anti-CD11c/ITGAX antibody and it appears that A00357-1 has been tested on human in both IHC and ELISA. Thus A00357-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-02-26

Question

Our lab were satisfied with the WB result of your anti-CD11c/ITGAX antibody. However we have observed positive staining in blood membrane using this antibody. Is that expected? Could you tell me where is ITGAX supposed to be expressed?

J. Li

Verified customer

Asked: 2018-11-05

Answer

According to literature, blood does express ITGAX. Generally ITGAX expresses in membrane. Regarding which tissues have ITGAX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2018-11-05

Question

Would anti-CD11c/ITGAX antibody A00357-1 work for ELISA with liver?

Verified Customer

Verified customer

Asked: 2018-05-25

Answer

According to the expression profile of liver, ITGAX is highly expressed in liver. So, it is likely that anti-CD11c/ITGAX antibody A00357-1 will work for ELISA with liver.

Boster Scientific Support

Answered: 2018-05-25

Question

We need to test anti-CD11c/ITGAX antibody A00357-1 on mouse liver for research purposes, then I may be interested in using anti-CD11c/ITGAX antibody A00357-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-05-07

Answer

The products we sell, including anti-CD11c/ITGAX antibody A00357-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-05-07

Question

See attached the WB image, lot number and protocol we used for liver using anti-CD11c/ITGAX antibody A00357-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-16

Question

We are currently using anti-CD11c/ITGAX antibody A00357-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-13

Answer

The anti-CD11c/ITGAX antibody (A00357-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-13

Question

Do you have a BSA free version of anti-CD11c/ITGAX antibody A00357-1 available?

Verified Customer

Verified customer

Asked: 2017-11-15

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD11c/ITGAX antibody A00357-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-11-15

Question

Is a blocking peptide available for product anti-CD11c/ITGAX antibody (A00357-1)?

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

We do provide the blocking peptide for product anti-CD11c/ITGAX antibody (A00357-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-06

Question

I would like using your anti-CD11c/ITGAX antibody for leukocyte migration studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-05-26

Answer

We appreciate your inquiry. This A00357-1 anti-CD11c/ITGAX antibody is validated on mouse thymus tissue, spleen tissue, tissue lysate, human tonsil tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-05-26

Question

Is this A00357-1 anti-CD11c/ITGAX antibody reactive to the isotypes of ITGAX?

J. Jones

Verified customer

Asked: 2013-07-18

Answer

The immunogen of A00357-1 anti-CD11c/ITGAX antibody is E. coli-derived human CD11c recombinant protein (Position: S161-T342). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-07-18

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00357-1

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A00357-1
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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