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Product Info Summary
| SKU: | A02187 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD158e KIR3DL1 Antibody
SKU/Catalog Number
A02187
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD158e KIR3DL1 Antibody catalog # A02187. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD158e KIR3DL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02187)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Killer cell immunoglobulin-like receptor 3DL1 at AA range: 21-70
Reactive Species
A02187 is reactive to KIR3DL1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
49.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02187 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of mouse-lung lysis using KIR3DL1 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000
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Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
Specific Publications For Anti-CD158e KIR3DL1 Antibody (A02187)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CD158e KIR3DL1 Antibody
Question
We are currently using anti-CD158e antibody A02187 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The anti-CD158e antibody (A02187) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-06
Question
My question regarding product A02187, anti-CD158e antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02187 anti-CD158e antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-07
Question
Is this A02187 anti-CD158e antibody reactive to the isotypes of KIR3DL1?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
The immunogen of A02187 anti-CD158e antibody is Synthesized peptide derived from the N-terminal region of human CD158e. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-17
Question
Would anti-CD158e antibody A02187 work on goat WB with natural killer cell?
L. Johnson
Verified customer
Asked: 2018-02-26
Answer
Our lab technicians have not tested anti-CD158e antibody A02187 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CD158e antibody A02187 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat natural killer cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood lymphocyte using anti-CD158e antibody A02187. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-30
