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Product Info Summary
| SKU: | A07190-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Interleukin-27 subunit beta EBI3 Antibody
SKU/Catalog Number
A07190-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Interleukin-27 subunit beta EBI3 Antibody (Catalog # A07190-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EBI3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Interleukin-27 subunit beta EBI3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07190-1)
Host
Rabbit
Contents
EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-EBI-3 antibody was raised against a peptide corresponding to 16 amino acids near the center of mature human EBI-3. The immunogen is located within amino acids 150 - 200 of EBI3.
Reactive Species
A07190-1 is reactive to EBI3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
25.4 kDa
Background of EBI3
EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07190-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 - 2 μg/mL; IF: 20 μg/mL ; IHC: 2.5 μg/mL;.
Antibody validated: Western Blot in human and rat samples; Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Rat Spleen Tissue
Loading: 15 μg of lysates per lane.
Antibodies: EBI-3 A07190-1 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Recombinant Protein Validation
Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane.
Antibodies: EBI-3 A07190-1, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane 1: 0.5 μg/mL,
Lane 2: 1 μg/mL,
Lane 3: 2 μg/mL,
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Immunohistochemistry Validation of EBI-3 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded Human Spleen tissue using anti-EBI-3 antibody (A07190-1) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of EBI-3 in Human Spleen Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling EBI-3 with A07190-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-Interleukin-27 subunit beta EBI3 Antibody (A07190-1)
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