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Product Info Summary
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Anti-IL27 Antibody Picoband™
Boster Bio Anti-IL27 Antibody Picoband™ catalog # A00857-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL27 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00857-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human IL27 recombinant protein (Position: F29-P243).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00857-1 is reactive to IL27 in Human
A00857-1 is guaranteed for ELISA, WB Boster Guarantee
Background of IL27
Interleukin 27 (IL-27) is a member of the IL-12 cytokine family. The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4 (+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4 (+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL27 using anti-IL27 antibody (A00857-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: recombinant human IL27 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL27 antigen affinity purified polyclonal antibody (Catalog # A00857-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL27 at approximately 60KD. The expected band size for IL27 is at 50KD.
Gene/Protein Information For IL27 (Source: Uniprot.org, NCBI)
Interleukin-27 subunit alpha
IL-27 p28 subunit; IL-27 subunit alpha; IL27; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A IL27 IL-27, IL-27AA, IL27p28, IL30, p28, IL27 interleukin 27 interleukin-27 subunit alpha|IL-27 p28 subunit|IL-27 subunit alpha|IL-27-A|IL27-A|interleukin-30*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on IL27, check out the IL27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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