Product Info Summary
SKU: | M00812-1 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371
SKU/Catalog Number
M00812-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 (Catalog # M00812-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00812-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM371
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human CD163
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human CD163
Reactive Species
M00812-1 is reactive to CD163 in Human
Reconstitution
Calculated molecular weight
125451 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00812-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of human placenta tissue lysate using anti-CD163 rabbit monoclonal antibody (Clone RM371) at a 1:2000 dilution.
Click image to see more details
Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human spleen tissue section using anti-CD163 rabbit monoclonal antibody (Clone RM371) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD163 (Source: Uniprot.org, NCBI)
Gene Name
CD163
Full Name
Scavenger receptor cysteine-rich type 1 protein M130
Weight
125451 MW
Alternative Names
Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130; CD163 M130, MM130, SCARI1 CD163 molecule scavenger receptor cysteine-rich type 1 protein M130|hemoglobin scavenger receptor|macrophage-associated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD163, check out the CD163 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD163: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 (M00812-1)
Hello CJ!
M00812-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Farag AGA,El Askary SA,Fathy WM,Elbassal F,Azzam AA,Tayel NR,Abdul Karim SS,Shehata WA. Relationship Between sCD163 and mCD163 and Their Implication in the Detection and Typing of Leprosy. Clin Cosmet Investig Dermatol.2020 Jun 2;13:379-389.doi:10.2147/CC
Species: Human
M00812-1 usage in article: APP:IHC, SAMPLE:BLOOD, DILUTION:NA
il-17 induces macrophages to M2-like phenotype via nF-%u03BAB
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371
Question
Can you help my question with product M00812-1, anti-CD163 Rabbit Monoclonal antibody, Clone#RM371. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00812-1 anti-CD163 Rabbit Monoclonal antibody, Clone#RM371, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-04
Question
We are currently using anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 (M00812-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-02
Question
We are interested in to test anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1 on human plasma for research purposes, then I may be interested in using anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The products we sell, including anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-04
Question
Do you have a BSA free version of anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1 available?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-24
Question
Our team were content with the WB result of your anti-CD163 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Based on literature, plasma does express CD163. Generally CD163 expresses in soluble cd163: secreted, cell membrane. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-09
Question
Our lab used your anti-CD163 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
I looked at the website and datasheets of our anti-CD163 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-09-16
Question
I see that the anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-20
Question
Would anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1 work for IHC with plasma?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
According to the expression profile of plasma, CD163 is highly expressed in plasma. So, it is likely that anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1 will work for IHC with plasma.
Boster Scientific Support
Answered: 2019-03-15
Question
Does M00812-1 anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Carter
Verified customer
Asked: 2018-10-26
Answer
You can see on the product datasheet, M00812-1 anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-26
Question
Is this M00812-1 anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 reactive to the isotypes of CD163?
N. Kulkarni
Verified customer
Asked: 2018-10-12
Answer
The immunogen of M00812-1 anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 is A peptide corresponding to the N-terminus of human CD163. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-12
Question
Is a blocking peptide available for product anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 (M00812-1)?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
We do provide the blocking peptide for product anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 (M00812-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-07
Question
We have seen staining in human liver. Any tips? Is anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 supposed to stain liver positively?
S. Krishna
Verified customer
Asked: 2017-08-15
Answer
From what I have seen in literature liver does express CD163. From what I have seen in Uniprot.org, CD163 is expressed in esophagus, spleen, plasma, liver, among other tissues. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-08-15
Question
Here is the WB image, lot number and protocol we used for plasma using anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 M00812-1. Please let me know if you require anything else.
A. Anderson
Verified customer
Asked: 2017-03-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-20
Question
I was wanting to use your anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 for IHC for human plasma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human plasma identification?
P. Li
Verified customer
Asked: 2016-05-10
Answer
You can see on the product datasheet, M00812-1 anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-10
Question
I am interested in using your anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 for acute-phase response studies. Has this antibody been tested with western blotting on human spleen tissue? We would like to see some validation images before ordering.
T. Miller
Verified customer
Asked: 2014-07-02
Answer
I appreciate your inquiry. This M00812-1 anti-CD163 Rabbit Monoclonal antibody, Clone#RM371 is tested on human spleen tissue, placenta tissue, tissue lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-07-02