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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human CD163 / M130 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CD163 / M130 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD163 / M130 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0715)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: G41-S1045
1.56 ng/ml - 100 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0715 is reactive to CD163 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK0715 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD163
CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
|EK0715-CAP||Anti-Human CD163 Pre-coated 96-well strip microplate||1|
|EK0715-ST||Human CD163 Standard||2 vials, 100 ng/tube|
|EK0715-DA||Human CD163 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CD163 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD163, Dilution ratio of 1:40, concentration in serum is around 500ng/ml.
Some articles we found to cite concentrations of CD163 in samples: 24548423, 25392926, 25156085, 24978196 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD163 / M130 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD163 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CD163 (Source: Uniprot.Org, NCBI)
Scavenger receptor cysteine-rich type 1 protein M130
CD163 antigenscavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; M130macrophage-associated antigen; MM130; RM3/1 CD163 M130, MM130, SCARI1 CD163 molecule scavenger receptor cysteine-rich type 1 protein M130|hemoglobin scavenger receptor|macrophage-associated antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CD163, check out the CD163 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD163: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0715 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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