Human CD163 / M130 ELISA Kit PicoKine™

Human CD163 / M130 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0715
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human CD163 / M130 ELISA Kit PicoKine™

See all CD163 products

SKU/Catalog Number

EK0715

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD163 / M130 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CD163 / M130 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0715)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G41-S1045

Sensitivity

<150 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0715 is reactive to CD163 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0715 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0715-CAP Anti-Human CD163 Pre-coated 96-well strip microplate 1
EK0715-ST Human CD163 Standard 2 vials, 100 ng/tube
EK0715-DA Human CD163 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.0120.0460.0960.2050.4490.9731.8642.469

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CD163, Dilution ratio of 1:40, concentration in serum is around 500ng/ml.

Some articles we found to cite concentrations of CD163 in samples: 24548423, 25392926, 25156085, 24978196 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD163 / M130 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3520114564467433681129448653
Standard deviation144.32526.973037.82195.34632.463357.05
CV (%)4.1%4.6%6.8%5.8%5.6%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD163 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135203403322230503298178.595.4%
Sample 21145611119132381207811972807.446.7%
Sample 344674429324230244756436661072.772.4%
*number of samples for each test n=16.

Gene/Protein Information For CD163 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q86VB7

Gene ID

9332

Gene Name

CD163

Full Name

Scavenger receptor cysteine-rich type 1 protein M130

Weight

125.451kDa

Alternative Names

CD163 antigenscavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; M130macrophage-associated antigen; MM130; RM3/1 CD163 M130, MM130, SCARI1 CD163 molecule scavenger receptor cysteine-rich type 1 protein M130|hemoglobin scavenger receptor|macrophage-associated antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD163, check out the CD163 Infographic

CD163 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD163: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0715 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

SIV Infection Impairs the Central Nervous System in Chinese Rhesus Macaques

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9 Customer Q&As for Human CD163 / M130 ELISA Kit PicoKine™

Question

Q: Can CD163 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Green

Verified customer

Asked: 2020-10-27

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-10-27

Question

Q: What is the optimal O.D. value for CD163 ELISA kit? I used your CD163 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD163 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-10-16

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD163 ELISA kit to have sensitivity of 150pg/ml, that means the minimum amount of CD163 that can be declared/interpreted as positive by the above standard is 150pg/ml.

Boster Scientific Support

Answered: 2020-10-16

Question

Q: how to thaw whole blood sample for CD163 ELISA after freezing?

P. Adams

Verified customer

Asked: 2020-09-27

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD163 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-09-27

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

M. Hill

Verified customer

Asked: 2020-09-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-09-14

Question

Could EDTA be used as an anticoagulant to collect plasma samples or do you advise to stick with the procedure in the EK0715 manual?

Verified customer

Asked: 2020-06-17

Answer

Usually we advise to follow the manual because EDTA plasma has chelating properties and may negatively affect results using the HSP70 ELISA Kit (High-Sensitivity) (EK7109).

Boster Scientific Support

Answered: 2020-06-17

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

A. Jackson

Verified customer

Asked: 2019-07-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-07-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-05-07

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-05-07

Question

Q: can I use EDTA plasma as samples in Human CD163 Picokine® ELISA Kit (Catalog # EK0715)?

Verified Customer

Verified customer

Asked: 2018-09-01

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD163 causing degradation of its protein structure. CD163 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD163 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-09-01

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

B. Chakrabarti

Verified customer

Asked: 2018-05-14

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-05-14

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