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Pecam1 antibody (A01513-3) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 85 Publications

a01513 3 pecam1 primary antibodies ihc testing 2

IHC analysis of Pecam1 using anti-Pecam1 antibody (A01513-3).
Pecam1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Pecam1 Antibody (A01513-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Anti-CD31/Pecam1 Antibody Picoband®

Name: Anti-CD31/Pecam1 Antibody Picoband®
Brand: Boster Bio
SKU: A01513-3
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

Pecam1 antibody

85 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, IHC, WB

Tested Applications

Pecam1 antibody was positively detected by WB in:

rat liver tissue, rat lung tissue, mouse lung tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Pecam1 antibody was positively detected by IHC in:

human placenta tissue, mouse lung tissue, rat liver tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Rat

Tested

Recommend

Mouse

Tested

Recommend

Human

Tested

Recommend

Product Information

Product Name

Anti-CD31/Pecam1 Antibody Picoband®
View all PECAM1 Antibodies

Form

Lyophilized

Uniprot

Q3SWT0

Gene ID

29583

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat CD31/Pecam1 recombinant protein (Position: Q41-E491).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

120-130 kDa

Calculated Molecular Weight

76.2 kDa

Background of Pecam1

CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily and this gene is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.

Cite This Product

Anti-CD31/Pecam1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01513-3)

Protocols

Western Blot protocol for Pecam1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Pecam1 antibody

Review Protocol

Publications

Electroacupuncture at LI11 and SP10 attenuates inflammation and pruritus in a rat model of passive cutaneous anaphylaxis-like urticaria via mast cell modulation related to transient receptor potential vanilloid 1

Published: 2026-07-15

Journal: Journal of Integrative Medicine-JIM

Application: IHC

Impact Factor: 5.20

Species: Rat

PMID: 42527214

SKU	A01513-3
Application	Immunofluorescence
Sample	Normal rat liver and alcoholic rat liver
Sample Processing Description	The samples consist of livers from normal SD rats and livers from SD rats with an alcoholic liver disease (ALD) model. The ALD model was established by gavaging the rats twice daily with 50% alcohol, while allowing them free access to alcoholic beverages, for a continuous period of 14 weeks.
Other Reagents	Goat Serum, DAPI, Anti-fade mounting medium
Primary Antibody	Anti-CD31/Pecam1 Antibody Picoband®
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	DyLight 594-conjugated Donkey Anti-Mouse IgG (H+L)
Secondary Incubation	1:500, 45 minutes in room temperature
Detection	Imaging system:Leica DM2500
Results Summary	CD31 is a marker of vascular endothelial cells. In normal liver, it is expressed in the endothelium of blood vessels, while liver sinusoidal endothelial cells (LSECs) show minimal expression. However, in alcoholic liver disease, the phenotype of LSECs changes and they begin to express CD31, leading to an overall increase in CD31 expression in the liver. This has also been confirmed by experimental results.

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Anti-CD31/Pecam1 Antibody Picoband®

Pecam1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Electroacupuncture at LI11 and SP10 attenuates inflammation and pruritus in a rat model of passive cutaneous anaphylaxis-like urticaria via mast cell modulation related to transient receptor potential vanilloid 1

Electroacupuncture at LI11 and SP10 is associated with alleviation of acute urticaria-like reactions in passive cutaneous anaphylaxis: an exploratory analysis of complement-related proteins and multiscale omics

Targeting DNA fragment extrusion: a new therapeutic avenue for CCl4-induced hepatic injury

Ozonated Water Modulates Cell Proliferation and Vascular Density in Solid Ehrlich Tumor of Mice

Multifunctional copper-based metal-organic frameworks/chitosan derivative/exosome composite sponge for skin healing

Effect of Artificial Dermal Scaffolds Compounded with Recombinant Human Epidermal Growth Factor on Wound Repair in Rats: A Comparative Study

Comprehensive analysis and validation of angiogenesis in vascular dementia from the perspective of diagnosis, prevention, and treatment

Salvianolic Acid B and Ginsenoside Rg1 Combination Attenuates Cerebral Edema Accompanying Glymphatic Modulation

Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

3D bioprinting of a dermal scaffold for full-thickness skin tissue regeneration

Phillygenin regulates the colorectal cancer tumor microenvironment by inhibiting hypoxia-inducible factor 1 alpha

Dual cross-linked self-healing hydrogel enhanced by dopamine nanoparticles and raffinose for wound healing

EPAC inhibitor suppresses angiogenesis and tumor growth of triple-negative breast cancer

Dual Network Hydrogels Based on PRP and SA Promote the Retention Rate and Vascularization of Transplanted Fat

Effect of 40 Hz light flicker on cognitive impairment and transcriptome of hippocampus in right unilateral common carotid artery occlusion mice

Exosomes Derived from E2F1–/– Adipose-Derived Stem Cells Promote Skin Wound Healing via miR-130b-5p/TGFBR3 Axis

G-Quadruplex/Hemin DNAzyme-Functionalized Silver Nanoclusters with Synergistic Antibacterial and Wound Healing Capabilities for Infected Wound Management

Antibacterial, conductive nanocomposite hydrogel based on dextran, carboxymethyl chitosan and chitosan oligosaccharide for diabetic wound therapy and health monitoring

Double Cross-Linked Biomimetic Hyaluronic Acid-Based Hydrogels with Thermo-Stimulated Self-Contraction and Tissue Adhesiveness for Accelerating Post-Wound Closure and Wound Healing

E2F1- deficient adipose-derived stem cells improve skin wound closure in mice by upregulating paracrine expression of VEGF and TGF-β1

Angiogenesis–Browning Interplay Mediated by Asprosin-Knockout Contributes to Weight Loss in Mice with Obesity

Pre-clinical evaluation of thermosensitive decellularized adipose tissue/platelet-rich plasma interpenetrating polymer network hydrogel for wound healing

Trimethylamine-N-oxide (TMAO) promotes balloon injury-induced neointimal hyperplasia via upregulating Beclin1 and impairing autophagic flux

Microglia Regulate Blood–Brain Barrier Integrity via MiR-126a-5p/MMP9 Axis during Inflammatory Demyelination

Intravoxel Incoherent Motion Diffusion-Weighted MR Imaging for Monitoring the Immune Response of Immunogenic Chemotherapy

Efficient starvation therapy with three-pathway blocking in combination with PTT/CDT for TME reversal and tumor apoptosis

Bioinspired design of mannose-decorated globular lysine dendrimers promotes diabetic wound healing by orchestrating appropriate macrophage polarization

An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties

Aerobic Exercise Attenuates Pressure Overload-Induced Cardiac Dysfunction through Promoting Skeletal Muscle Microcirculation and Increasing Muscle Mass

Icebreaker-inspired Janus nanomotors to combat barriers in the delivery of chemotherapeutic agents

Effect of Electrical Stimulation of Cervical Sympathetic Ganglia on Intraocular Pressure Regulation According to Different Circadian Rhythms in Rats

Near infrared light-triggered on-demand Cur release from Gel-PDA@Cur composite hydrogel for antibacterial wound healing

Autologous bionic tissue for inguinal hernia repair

Sodium Tanshinone IIA sulfonate improves post-ischemic angiogenesis in hyperglycemia

Attenuation of hyperoxic acute lung injury by Lycium barbarum polysaccharide via inhibiting NLRP3 inflammasome

Ginsenoside Rh2 Inhibits Angiogenesis in Prostate Cancer by Targeting CNNM1

Recombinant human growth hormone (rhGH) treatment of MKN-45 xenograft mice improves nutrition status and strengthens immune function without promoting tumor growth

Naringin enhances endothelial progenitor cell (EPC) proliferation and tube formation capacity through the CXCL12/CXCR4/PI3K/Akt signaling pathway

Endothelial progenitor cells contribute to neovascularization of non-small cell lung cancer via histone deacetylase 7-mediated cytoskeleton regulation and angiogenic genes transcription

Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats

Freeze-dried and irradiated allograft bone combined with fresh autologous coagula promotes angiogenesis in an ectopic bone allograft implantation model

Involvement of aberrantly activated HOTAIR/EZH2/miR-193a feedback loop in progression of prostate cancer

Acacetin attenuates mice endotoxin-induced acute lung injury via augmentation of heme oxygenase-1 activity

Peptide-Modified Chitosan Hydrogels Accelerate Skin Wound Healing by Promoting Fibroblast Proliferation, Migration, and Secretion.

Vimentin is a crucial target for anti-metastasis therapy of nasopharyngeal carcinoma

WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects

Immunomodulatory activity of interleukin-27 in human chronic periapical diseases

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/β-catenin signaling

Peptide-modified PELCL electrospun membranes for regulation of vascular endothelial cells

Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos

Angiotensin-Converting Enzyme Inhibition Attenuates Lipopolysaccharide-Induced Lung Injury by Regulating the Balance Between Angiotensin-Converting Enzyme and Angiotensin-Converting Enzyme 2 and Inhibiting Mitogen-Activated Protein Kinase Activation

Macrophage peroxisome proliferator-activated receptor γ deficiency delays skin wound healing through impairing apoptotic cell clearance in mice

SKLB-M8 Induces Apoptosis Through the AKT/mTOR Signaling Pathway in Melanoma Models and Inhibits Angiogenesis With Decrease of ERK1/2 Phosphorylation

Expression of nerve growth factor and hypoxia inducible factor-1α and its correlation with angiogenesis in non-small cell lung cancer

Different Angiogenic Abilities of Self-Setting Calcium Phosphate Cement Scaffolds Consisting of Different Proportions of Fibrin Glue

MicroRNA-885-3p inhibits the growth of HT-29 colon cancer cell xenografts by disrupting angiogenesis via targeting BMPR1A and blocking BMP/Smad/Id1 signaling

Expression of IL-17A and IL-17F in lipopolysaccharide-induced acute lung injury and the counteraction of anisodamine or methylprednisolone

Enhanced external counterpulsation inhibits endothelial apoptosis via modulation of BIRC2 and Apaf-1 genes in porcine hypercholesterolemia

SRPK1 Dissimilarly Impacts on the Growth, Metastasis, Chemosensitivity and Angiogenesis of Glioma in Normoxic and Hypoxic Conditions

Influence of Mesenchymal Stem Cells with Endothelial Progenitor Cells in Co-culture on Osteogenesis and Angiogenesis: An In Vitro Study

Electrosprayed Microparticles with Loaded pDNA-Calcium Phosphate Nanoparticles to Promote the Regeneration of Mature Blood Vessels

Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF

Pneumatic mold-aided construction of a three-dimensional hydrogel microvascular network in an integrated microfluidics and assay of cancer cell adhesion onto the endothelium

Cotransfection of Survivin and CD44v3 Short Hairpin RNAs Affects Proliferation, Apoptosis, and Invasiveness of Colorectal Cancer

Proteomics Identification of Annexin A2 as a Key Mediator in the Metastasis and Proangiogenesis of Endometrial Cells in Human Adenomyosis *