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Product Info Summary
| SKU: | M01513-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247
SKU/Catalog Number
M01513-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 (Catalog # M01513-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01513-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM247
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the cytoplasmic domain of human CD31
Cross-reactivity
This antibody reacts to human CD31 (Platelet endothelial cell adhesion molecule, PECAM-1).
Reactive Species
M01513-3 is reactive to PECAM1 in Human
Calculated molecular weight
82.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01513-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2500 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of Jurkat cell lysate, using Anti-CD31 RM247 at a 1:1000 dilution, showed CD31 (~130 kDa) expression in Jurkat cells.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CD31 RM247 at a 1:2500 dilution.
Specific Publications For Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 (M01513-3)
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Customer Reviews
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5 Customer Q&As for Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247
Question
We are currently using anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 M01513-3 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 (M01513-3) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-30
Question
We bought anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
I looked at the website and datasheets of our anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-11-28
Question
We have observed staining in human leukemic t-cell. What should we do? Is anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
From literature leukemic t-cell does express PECAM1. From Uniprot.org, PECAM1 is expressed in tendon of biceps brachii, lung, brain, platelet, liver, leukemic t-cell, among other tissues. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2018-10-03
Question
We are interested in using your anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 for rho protein signal transduction studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
Thanks for your inquiry. This M01513-3 anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 is validated on jurkat cell lysate, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-09-07
Question
Our lab were satisfied with the WB result of your anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone
C. Krishna
Verified customer
Asked: 2017-08-01
Answer
From what I have seen in literature, liver does express PECAM1. Generally PECAM1 expresses in cell membrane. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2017-08-01
