Human VEGF-C ELISA Kit PicoKine™

Human VEGF-C ELISA Kit PicoKine™ (96 Tests). Quantitate Human VEGFC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. Cited in 13 publication(s).

Product Info Summary

SKU: EK0588
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human VEGF-C ELISA Kit PicoKine™

See all VEGF-C products

SKU/Catalog Number

EK0588

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human VEGF-C ELISA Kit PicoKine™ (96 Tests). Quantitate Human VEGFC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human VEGF-C ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0588)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T103-R227

Sensitivity

<3 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0588 is reactive to VEGFC in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0588 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0588-CAP Anti-Human VEGFC Pre-coated 96-well strip microplate 1
EK0588-ST Human VEGFC Standard 2 vials, 10 ng/tube
EK0588-DA Human VEGFC Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.1100.1750.2170.2980.4820.7921.4132.357

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect VEGF-C, Dilution ratio of 1:1, concentration in serum is 500-1250pg/ml.

Some articles we found to cite concentrations of VEGF-C in samples: 22855190 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human VEGF-C ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)868451351898361341
Standard deviation4.0456.6172.955.5171.8976.43
CV (%)4.7%6.7%5.4%6.2%8.6%5.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of VEGF-C in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 186849088872.232.5%
Sample 284574581674878843.245.4%
Sample 31351128013491264131139.413%
*number of samples for each test n=16.

Gene/Protein Information For VEGFC (Source: Uniprot.Org, NCBI)

Uniprot ID

P49767

Gene ID

7424

Gene Name

VEGFC

Full Name

Vascular endothelial growth factor C

Weight

46.883kDa

Superfamily

PDGF/VEGF growth factor family

Alternative Names

Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM VEGFC Flt4-L, LMPH1D, LMPHM4, VRP vascular endothelial growth factor C vascular endothelial growth factor C|FLT4 ligand DHM|vascular endothelial growth factor-related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VEGFC, check out the VEGFC Infographic

VEGFC infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VEGFC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0588 has been cited in 13 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin
Species: Human

Zhu, G., Lin, C., Cheng, Z., Wang, Q., Hoffman, R. M., Singh, S. R., ... & Ye, J. (2020). TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140. Cancer Genomics-Proteomics, 17(1), 23-33.

RNA interference (RNAi)-mediated vascular endothelial growth factor-C (VEGF-C) reduction interferes with lymphangiogenesis and enhances epirubicin sensitivity of breast cancer cells.

Lipopolysaccharide increases the release of VEGF-C that enhances cell motility and promotes lymphangiogenesis and lymphatic metastasis through the TLR4- NF-?B/JNK pathways in colorectal cancer

SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin

Wang J, Zhao W, Guo Y, Zhang B, Xie Q, Xiang D, Gao J, Wang B, Chen Z. Oncology. 2009;76(6):420-9. Doi: 10.1159/000216837. Epub 2009 May 5. The Expression Of Rna-Binding Protein Hur In Non-Small Cell Lung Cancer Correlates With Vascular Endothelia...

Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.

Zhang X, Ding L, Diao Z, Yan G, Sun H, Hu Y. Am J Reprod Immunol. 2012 Mar;67(3):216-23. Doi: 10.1111/J.1600-0897.2011.01080.X. Epub 2011 Oct 24. Cyr61 Modulates The Vascular Endothelial Growth Factor C Expression Of Decidual Nk Cells Via Pi3K/Akt...

Chuangui C, Peng T, Zhentao Y. Pathol Oncol Res. 2012 Oct;18(4):1021-7. Epub 2012 Apr 29. The Expression Of High Mobility Group Box 1 Is Associated With Lymph Node Metastasis And Poor Prognosis In Esophageal Squamous Cell Carcinoma.

Xu Yf, Ge Fj, Han B, Yang Xq, Su H, Zhao Ac, Zhao Mh, Yang Yb, Yang J. World J Gastroenterol. 2015 Mar 21;21(11):3256-65. Doi: 10.3748/Wjg.V21.I11.3256. High-Mobility Group Box 1 Expression And Lymph Node Metastasis In Intrahepatic Cholangiocarcin...

Li Jt, Wang Lf, Zhao Yl, Yang T, Li W, Zhao J, Yu F, Wang L, Meng Yl, Liu Nn, Zhu Xs, Gao Cf, Jia Lt, Yang Ag. Breast Cancer Res. 2014 Oct 14;16(5):454. Doi: 10.1186/S13058-014-0454-2. Nuclear Factor Of Activated T Cells 5 Maintained By Hotair Sup...

Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.

Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ...

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8 Customer Q&As for Human VEGF-C ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for VEGF-C ELISA kit? I used your VEGF-C ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain VEGF-C even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-09-13

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this VEGF-C ELISA kit to have sensitivity of 3pg/ml, that means the minimum amount of VEGF-C that can be declared/interpreted as positive by the above standard is 3pg/ml.

Boster Scientific Support

Answered: 2020-09-13

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

L. Taylor

Verified customer

Asked: 2019-11-10

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-11-10

Question

Q: how to proceed with the analysis of ELISA data? I have obtained VEGF-C level in plasma.

R. Kumar

Verified customer

Asked: 2019-10-19

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-10-19

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-06-04

Question

Q: Can VEGF-C ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

K. Dayal

Verified customer

Asked: 2019-03-31

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-03-31

Question

Q: if the enzyme conjugated VEGF-C antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the VEGF-C antigen?

M. Surve

Verified customer

Asked: 2019-01-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-01-16

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-04-17

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-04-17

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2017-03-01

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-03-01

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