Human PAI-1 ELISA Kit PicoKine™

Human PAI-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINE1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0859
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ).

Product Name

Human PAI-1 ELISA Kit PicoKine™

See all Serpin E1/PAI-1 products

SKU/Catalog Number

EK0859

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PAI-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINE1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human PAI-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0859)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: sf21; Immunogen sequence: S22-P402

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0859 is reactive to SERPINE1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ).

Application Guarantee

EK0859 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0859-CAP Anti-Human SERPINE1 Pre-coated 96-well strip microplate 1
EK0859-ST Human SERPINE1 Standard 2 vials, 10 ng/tube
EK0859-DA Human SERPINE1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0330.1550.2790.4200.6491.0431.4602.024

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PAI-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2031208549619812445980
Standard deviation12.3871.27423.1915.0495.78520.26
CV (%)6.1%7.7%%7.7%7.6%7.7%8.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Serpin E1/PAI-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120321821823421810.965%
Sample 21208130813241403131069.375.2%
Sample 3549651846077569656133245.7%
*number of samples for each test n=16.

Gene/Protein Information For SERPINE1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05121

Gene ID

5054

Gene Name

SERPINE1

Full Name

Plasminogen activator inhibitor 1

Weight

45.06kDa

Superfamily

serpin family

Alternative Names

Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1 SERPINE1 PAI, PAI-1, PAI1, PLANH1 serpin family E member 1 plasminogen activator inhibitor 1|endothelial plasminogen activator inhibitor|serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1|serpin E1|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SERPINE1, check out the SERPINE1 Infographic

SERPINE1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SERPINE1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0859 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Oligodeoxyribonucleotides derived from salmon sperm DNA: an alternative to defibrotide

Nilotinib Does Not Alter the Secretory Functions of Carotid Artery Endothelial Cells in a Prothrombotic or Antithrombotic Fashion

The Association of Plasminogen Activator Inhibitor Type 1 (PAI-1) Level and PAI-1 4G/5G Gene Polymorphism with the Formation and the Grade of Endometrial Cancer

The prevalence of 4G/5G polymorphism of plasminogen activator inhibitor-1 (PAI-1) gene in central serous chorioretinopathy and its association with plasma PAI-1 levels.

Associations between homocysteine metabolism related SNPs and carotid intima-media thickness: a Chinese sib pair study

Disequilibrium of Blood Coagulation and Fibrinolytic System in Patients With Coronary Artery Ectasia

The lost correlation between heat shock protein 70 (HSPA1A) and plasminogen activator inhibitor-1 in patients with type 2 diabetes and albuminuria

Sun Y, Yang D, Xi L, Chen Y, Fu L, Sun K, Yin J, Li X, Liu S, Qin Y, Liu M, Hou Y. Cell Death Dis. 2018 Jan 26;9(2):122. doi: 10.1038/s41419-017-0133-1. Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity cha...

He, S., Le, N.A., Frediani, J.K., Winterberg, P.D., Jin, R., Liverman, R.,..., & Vos, M.B. (2017). Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. ...

Chen Y, Lu Zj, Yang Y, Lu Gp, Chen Wm, Zhang Le. Thromb Res. 2015 Jul;136(1):131-8. Doi: 10.1016/J.Thromres.2015.04.018. Epub 2015 Apr 22. Suppression Of Plasminogen Activator Inhibitor-1 By Inhaled Nitric Oxide Attenuates The Adverse Effects Of H...

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7 Customer Q&As for Human PAI-1 ELISA Kit PicoKine™

Question

Q: Can Serpin E1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-03-11

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Serpin E1 using the EK0859 Human Serpin E1 Picokine® ELISA Kit?

W. Mangal

Verified customer

Asked: 2019-01-17

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-01-17

Question

Q: is it okay to use EDTA plasma as samples in Human Serpin E1 Picokine® ELISA Kit (Catalog # EK0859)?

Verified Customer

Verified customer

Asked: 2019-01-05

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Serpin E1 causing disruption of its protein structure. Serpin E1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Serpin E1 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-01-05

Question

Q: how to proceed with the analysis of ELISA data? I have performed Serpin E1 level in serum.

Verified Customer

Verified customer

Asked: 2018-09-03

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-09-03

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-08-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-08-14

Question

Q: What is the optimal O.D. value for Serpin E1 ELISA kit? I used your Serpin E1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Serpin E1 even though the O.D. values are not very high?

W. Carter

Verified customer

Asked: 2017-07-22

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin E1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Serpin E1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2017-07-22

Question

Q: if the enzyme conjugated Serpin E1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin E1 antigen?

K. Kumar

Verified customer

Asked: 2015-07-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2015-07-16

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