Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300

Boster Bio Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300 catalog # M00885-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 25 publication(s).

Product Info Summary

SKU: M00885-2
Size: 100uL
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300

See all CD34 products

SKU/Catalog Number

M00885-2

Size

100uL

Description

Boster Bio Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300 catalog # M00885-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00885-2)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM300

Immunogen

A peptide corresponding to the C-terminus of human CD34

Reactive Species

M00885-2 is reactive to CD34 in Human, Mouse, Rat

Applications

M00885-2 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:100-1:200 dilution;
Western Blot (WB): 1:100-1:200 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For CD34 (Source: Uniprot.Org, NCBI)

Uniprot ID

P28906

Gene ID

947

Gene Name

CD34

Full Name

Hematopoietic progenitor cell antigen CD34

Weight

40.716kDa

Superfamily

CD34 family

Alternative Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 CD34 CD34 molecule hematopoietic progenitor cell antigen CD34|CD34 antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD34, check out the CD34 Infographic

CD34 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD34: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00885-2 has been cited in 25 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Assefa F,Lim J,Kim JA,Ihn HJ,Lim S,Nam SH,Bae YC,Park EK. Secretoneurin, a Neuropeptide, Enhances Bone Regeneration in a Mouse Calvarial Bone Defect Model. Tissue Eng Regen Med.2020 Nov 3.doi: 10.1007/s13770-020-00304-1.Epub ahead of print.PMID:33145742.
Species: Mouse
M00885-2 usage in article: APP:IHC, SAMPLE:CALVARIAL BONE, DILUTION:1:500

Dl-3-n-Butylphthalide treatment enhances hemodynamics and ameliorates memory deficits in rats with chronic cerebral hypoperfusion

Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1? expression in the transition zone

Heparin Impairs Angiogenesis through Inhibition of MicroRNA-10b

Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Incomplete radiofrequency ablation accelerates proliferation and angiogenesis of residual lung carcinomas via HSP70/HIF-1?

Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair

Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury

Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury

Modulating hair follicle size with?Wnt10b-DKK1?pair during hair regeneration

Li D, Wei X, Xie K, Chen K, Li J, Fang J. Br J Cancer. 2014 Jul 8;111(1):68-77. Doi: 10.1038/Bjc.2014.282. Epub 2014 May 29. A Novel Decoy Receptor Fusion Protein For Fgf-2 Potently Inhibits Tumour Growth.

Luo Bh, Xiong F, Wang Jp, Li Jh, Zhong M, Liu Ql, Luo Gq, Yang Xj, Xiao N, Xie B, Xiao H, Liu Rj, Dong Cs, Wang Ks, Wen Jf. Plos One. 2014 Jun 19;9(6):E99922. Doi: 10.1371/Journal.Pone.0099922. Ecollection 2014. Epidermal Growth Factor-Like Domain...

Fu W, Lv C, Zhuang W, Chen D, Lv E, Li F, Wang X. Neural Regen Res. 2013 Feb 15;8(5):427-34. Doi: 10.3969/J.Issn.1673-5374.2013.05.006. An Effective Inducer Of Dopaminergic Neuron-Like Differentiation.

Qiu W, Lei M, Li J, Wang N, Lian X. Int J Med Sci. 2014 Jul 30;11(10):1022-8. Doi: 10.7150/Ijms.8383. Ecollection 2014. Activated Hair Follicle Stem Cells And Wnt/??-Catenin Signaling Involve In Pathnogenesis Of Sebaceous Neoplasms.

Zhu Sk, Zhou Y, Cheng C, Zhong S, Wu Hq, Wang B, Fan P, Xiong Jx, Yang Hj, Wu Hs. Mol Clin Oncol. 2014 Nov;2(6):973-981. Epub 2014 Jul 9. Overexpression Of Membrane-Type 2 Matrix Metalloproteinase Induced By Hypoxia-Inducible Factor-1?? In Pancrea...

Jing Z, Shi C, Zhu L, Xiang Y, Chen P, Xiong Z, Li W, Ruan Y, Huang L. J Cereb Blood Flow Metab. 2015 Aug;35(8):1249-59. Doi: 10.1038/Jcbfm.2015.55. Epub 2015 Apr 8. Chronic Cerebral Hypoperfusion Induces Vascular Plasticity And Hemodynamics But A...

Chen J, Jia Zy, Ma Zl, Wang Yy, Teng Gj. Plos One. 2011;6(6):E20790. Doi: 10.1371/Journal.Pone.0020790. Epub 2011 Jun 24. In Vivo Serial Mr Imaging Of Magnetically Labeled Endothelial Progenitor Cells Homing To The Endothelium Injured Artery In Mice.

Zhou J, Wang L, Xu X, Tu Y, Qin S, Yin Y. Oncol Lett. 2012 Nov;4(5):976-980. Epub 2012 Aug 8. Antitumor Activity Of Endostar Combined With Radiation Against Human Nasopharyngeal Carcinoma In Mouse Xenograft Models.

Kong J, Kong J, Pan B, Ke S, Dong S, Li X, Zhou A, Zheng L, Sun Wb. Plos One. 2012;7(5):E37266. Doi: 10.1371/Journal.Pone.0037266. Epub 2012 May 15. Insufficient Radiofrequency Ablation Promotes Angiogenesis Of Residual Hepatocellular Carcinoma Vi...

Huang Xf, Yuan Sj, Yang C. Mol Med Rep. 2012 Sep;6(3):547-52. Doi: 10.3892/Mmr.2012.974. Epub 2012 Jun 29. Effects Of Total Flavonoids From Drynaria??fortunei On The Proliferation And Osteogenic Differentiation Of Rat Dental Pulp Stem ...

Zhao Cl, Zhang Gp, Xiao Zz, Ma Zk, Lei Cp, Song Sy, Feng Yy, Zhao Yc, Feng Xs. J Breast Cancer. 2015 Jun;18(2):126-33. Doi: 10.4048/Jbc.2015.18.2.126. Epub 2015 Jun 26. Recombinant Human Granulocyte Colony-Stimulating Factor Promotes Preinvasive A...

Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.

Jiang Jt, Zhang Lf, Zhou B, Zhang Sq, Li Sm, Zhang W, Zhang J, Qiao Z, Kong Rr, Ma Yf, Chen S. Asian Pac J Cancer Prev. 2012;13(7):3379-83. Relationships Of Upa And Vegf Expression In Esophageal Cancer And Microvascular Density With Tumorous Invas...

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16 Customer Q&As for Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300

Question

Our lab were content with the WB result of your anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

From what I have seen in literature, bone marrow does express CD34. Generally CD34 expresses in membrane. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-31

Question

I was wanting to use to test anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 on human megakaryoblast for research purposes, then I may be interested in using anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

The products we sell, including anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-11

Question

We have been able to see staining in human bone marrow. Are there any suggestions? Is anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 supposed to stain bone marrow positively?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

From what I have seen in literature bone marrow does express CD34. From what I have seen in Uniprot.org, CD34 is expressed in esophagus, bone marrow, megakaryoblast, heart, placenta, skin, among other tissues. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-01-31

Question

I am interested in using your anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 for metanephric glomerular mesangial cell differentiation studies. Has this antibody been tested with western blotting on human kidney tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

We appreciate your inquiry. This M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 is tested on mouse spleen tissue, tissue lysate, human kidney tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-24

Question

See attached the WB image, lot number and protocol we used for megakaryoblast using anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-06

Question

I was wanting to use your anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 for WB for human megakaryoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human megakaryoblast identification?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

It shows on the product datasheet, M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human megakaryoblast in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-29

Question

We are currently using anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

The anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 (M00885-2) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-23

Question

I see that the anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 works with WB, what is the protocol used to produce the result images on the product page?

G. Jones

Verified customer

Asked: 2018-06-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-06-13

Question

I have a question about product M00885-2, anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Li

Verified customer

Asked: 2018-01-23

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-23

Question

Is this M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 reactive to the isotypes of CD34?

Verified Customer

Verified customer

Asked: 2018-01-10

Answer

The immunogen of M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 is A peptide corresponding to the C-terminus of human CD34. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-10

Question

Is there a BSA free version of anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 available?

Verified Customer

Verified customer

Asked: 2017-08-03

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-08-03

Question

Does M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

R. Parker

Verified customer

Asked: 2017-06-27

Answer

As indicated on the product datasheet, M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-06-27

Question

Is a blocking peptide available for product anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 (M00885-2)?

C. Krishna

Verified customer

Asked: 2017-02-13

Answer

We do provide the blocking peptide for product anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 (M00885-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-02-13

Question

Would anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 work for WB with megakaryoblast?

E. Mitchell

Verified customer

Asked: 2015-12-09

Answer

According to the expression profile of megakaryoblast, CD34 is highly expressed in megakaryoblast. So, it is likely that anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 will work for WB with megakaryoblast.

Boster Scientific Support

Answered: 2015-12-09

Question

We ordered your anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone

B. Roberts

Verified customer

Asked: 2014-07-03

Answer

I took a look at the website and datasheets of our anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2014-07-03

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for megakaryoblast using anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2. Let me know if you need anything else.

J. Bhatt

Verified customer

Asked: 2013-06-19

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-06-19

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M00885-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$382.00

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