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Product Info Summary
| SKU: | PB9371 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD36 Antibody Picoband®
SKU/Catalog Number
PB9371
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD36 Antibody Picoband® catalog # PB9371. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD36 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9371)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD36, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9371 is reactive to CD36 in Human, Mouse, Rat
Observed Molecular Weight
88 kDa
Calculated molecular weight
53.1 kDa
Background of CD36
CD36 (cluster of differentiation 36), also known as FAT (fatty acid translocase), FAT/CD36, (FAT)/CD36, SCARB3, GP88, glycoprotein IV (gpIV), and glycoprotein IIIb (gpIIIb), is anintegral membrane protein found on the surface of many cell types in vertebrate animals. CD36 is a member of the class B scavenger receptor family of cell surface proteins. It is mapped to 7q21.11. And CD36 binds many ligands including collagen, thrombospondin, erythrocytes parasitized with Plasmodium falciparum, oxidized low density lipoprotein, native lipoproteins, oxidized phospholipids, and long-chain fatty acids. In addition, CD36 function in long-chain fatty acid uptake and signaling can be irreversibly inhibited by sulfo-N-succinimidyl oleate (SSO), which binds lysine 164 within a hydrophobic pocked shared by several CD36 ligands, e.g. fatty acid and oxLDL.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9371 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Liver Tissue, Rat Cardiac Muscle Tissue, Mouse Liver Tissue, Mouse Cardiac Muscle Tissue, SMMC Whole Cell
IHC: Rat Spleen Tissue, human Lung cancer tissue
Validation Images & Assay Conditions
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Anti-CD36 Picoband antibody, PB9371, IHC(P)
IHC(P): Rat Spleen Tissue
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Anti-CD36 Picoband antibody, PB9371, Western blotting
All lanes: Anti CD36 (PB9371) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 88KD
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IHC analysis of Galectin 1 using anti-Galectin 1 antibody (PB9371).
Galectin 1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Galectin 1 Antibody (PB9371) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-CD36 Antibody Picoband® (PB9371)
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8 Customer Q&As for Anti-CD36 Antibody Picoband®
Question
I would like to know about the Anti-CD36 Antibody Picoband, how long would the cells be treated for the blocking study?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We would recommend for the Anti-CD36 Antibody Picoband concentration is 2ug/ml, however the incubation time will need to be determined by the investigator depending on the biomarkers they are measuring.
Boster Scientific Support
Answered: 2020-04-14
Question
We were well pleased with the WB result of your anti-CD36 antibody. However we have been able to see positive staining in skeletal muscle cell membrane using this antibody. Is that expected? Could you tell me where is CD36 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
From literature, skeletal muscle does express CD36. Generally CD36 expresses in cell membrane. Regarding which tissues have CD36 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Liver, Pubmed ID: 19159218
Milk, Pubmed ID: 18780401
Placenta, Pubmed ID: 2473841
Platelet, Pubmed ID: 16263699, 2468669, 11668637, 7505064, 7518447, 12853948
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-18
Question
I am interested in using your anti-CD36 antibody for sensory perception of taste studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We appreciate your inquiry. This PB9371 anti-CD36 antibody is validated on rat liver tissue, tissue lysate, cardiac muscle tissue, mouse liver tissue, smmc whole cell lysate, spleen tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-09
Question
We are currently using anti-CD36 antibody PB9371 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The anti-CD36 antibody (PB9371) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
We have observed staining in human liver. Are there any suggestions? Is anti-CD36 antibody supposed to stain liver positively?
C. Edwards
Verified customer
Asked: 2019-09-02
Answer
Based on literature liver does express CD36. Based on Uniprot.org, CD36 is expressed in adipose tissue, placenta, platelet, skeletal muscle, adipocyte, milk, liver, among other tissues. Regarding which tissues have CD36 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Liver, Pubmed ID: 19159218
Milk, Pubmed ID: 18780401
Placenta, Pubmed ID: 2473841
Platelet, Pubmed ID: 16263699, 2468669, 11668637, 7505064, 7518447, 12853948
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-02
Question
We purchased anti-CD36 antibody for WB on skeletal muscle a few years ago. I am using human, and I plan to use the antibody for IHC next. I would like examining skeletal muscle as well as milk in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
I took a look at the website and datasheets of our anti-CD36 antibody and I see that PB9371 has been tested on human in both WB and IHC. Thus PB9371 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-26
Question
I would like to know what the antigen recognition site for this antibody PB9371, is it in the amino acid peptide sequence of CD36? Or peptide modified by glycosylation
Verified Customer
Verified customer
Asked: 2016-06-16
Answer
The immunogen for antibody PB9371 is 20 weeks old fetal erythrocytes and we have not further mapped the epitope for this antibody.
Boster Scientific Support
Answered: 2016-06-16
Question
How long is this PB9371 antibody stable at 4 degrees?
Verified Customer
Verified customer
Asked: 2013-06-25
Answer
The product PB9371 antibody must be stored at 4degrees under recommended conditions and this product would be stable for one year from the date of receipt.
Boster Scientific Support
Answered: 2013-06-25
