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- Table of Contents
CD34 antibody (PB9053) performs well in ELISA, IF, IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IF analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD34 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-CD34 Antibody Picoband®
CD34 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, IF, IHC, WB |
Tested Applications
CD34 antibody was positively detected by WB in:
human placenta tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CD34 antibody was positively detected by IHC in:
human lymphoma tissue, human gastric cancer tissue, human glioma tissue, human ovarian cancer tissue, human placenta tissue, human liver cancer tissue, rat kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CD34 antibody was positively detected by IF in:
human colon cancer tissue, human placenta tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD34 Antibody Picoband®
View all CD34 Antibodies
View all CD34 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD34 recombinant protein (Position: T151-L385). Human CD34 shares 79% amino acid (aa) sequence identity with mouse CD34.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
105 kDa
Calculated Molecular Weight
40.7 kDa
Background of CD34
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.
Cite This Product
Anti-CD34 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9053)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Dog
83%
Moderate Homology
Rabbit
81%
Moderate Homology
Mouse
79%
Moderate Homology
Sheep
71%
Moderate Homology
Cow
70%
Moderate Homology
Rat
52%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CD34 antibody
Immunohistochemistry (IHC) protocol for CD34 antibody
Immunofluorescence (IF) protocol for CD34 antibody
Publications
Application:
Species:
Exploring miR-21 as a key regulator in two distinct approaches of bone marrow stromal cells differentiation into Schwann-like cells
Published: 2024-05-23
Journal: SYNAPSE
Application: IF
Impact Factor: 1.60
Species: Rat
PMID: 38779935
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties
Published: 2021-11-19
Journal: BIOMATERIALS
Application: IHC
Impact Factor: 12.48
Species: Rat
PMID: 34823885
The Effects of Inactive Platelet-rich Plasma at Different Injection Time on Prefabricated Flap Viability in Rabbits
Published: 2021-06-01
Journal: ANNALS OF PLASTIC SURGERY
Application: IHC
Impact Factor: 1.54
Species: Rabbit
PMID: 34002724
Construction of Urokinase-Type Plasminogen Activator Receptor-Targeted Heterostructures for Efficient Photothermal Chemotherapy against Cervical Cancer To Achieve Simultaneous Anticancer and Antiangiogenesis
Published: 2019-10-07
Journal: ACS Applied Materials & Interfaces
Application: IHC
Impact Factor: 8.46
Species: Human
PMID: 31588724
Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration
Published: 2018-08-28
Journal: NPG Asia Materials
Application: IF
Impact Factor: 7.21
Species: Rabbit
Inhibiting trophoblast PAR-1 overexpression suppresses sFlt-1-induced anti-angiogenesis and abnormal vascular remodeling: a possible therapeutic approach for preeclampsia
Published: 2018-01-09
Journal: MOLECULAR HUMAN REPRODUCTION
Application: IHC
Impact Factor: 3.45
Species: Human
PMID: 29325127
Overexpression of Inducible Nitric Oxide Synthase Impairs the Survival of Bone marrow Stem Cells Transplanted into Rat Infarcted Myocardium
Published: 2014-04-26
Journal: LIFE SCIENCES
Application: IF
Impact Factor: 2.30
Species: Rat
PMID: 24780316
Histopathological studies of microtubule disassembling agent-induced myocardial lesions in rats
Published: 2012-11-03
Journal: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
Application: IHC
Impact Factor: 2.78
Species: Rat
PMID: 23131582
Experimental study on inhibitory effects of diallyl sulfide on growth and invasion of human osteosarcoma MG-63 cells
Published: 2012-08-11
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Impact Factor: 0.39
PMID: 22886974
Relationships of uPA and VEGF Expression in Esophageal Cancer and Microvascular Density with Tumorous Invasion and Metastasis
Published: 2012-07-31
Journal: Asian Pacific Journal of Cancer Prevention
Application: IHC
Impact Factor: 0.66
Species: Human
PMID: 22994764
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Product Promise
We promise Anti-CD34 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9053
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026