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Product Info Summary
| SKU: | A00137 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD14 Antibody Picoband™
SKU/Catalog Number
A00137
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD14 Antibody Picoband™ catalog # A00137. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD14 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00137)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD14 recombinant protein (Position: S16-A260).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00137 is reactive to CD14 in Mouse, Rat
Applications
A00137 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
40.076kDa
Background of CD14
CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD14 using anti-CD14 antibody (A00137).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 antigen affinity purified polyclonal antibody (Catalog # A00137) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD14 at approximately 50KD. The expected band size for CD14 is at 40KD.
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Figure 2. IHC analysis of CD14 using anti-CD14 antibody (A00137).
CD14 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD14 Antibody (A00137) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD14 using anti-CD14 antibody (A00137).
CD14 was detected in paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD14 Antibody (A00137) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CD14 using anti-CD14 antibody (A00137).
CD14 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD14 Antibody (A00137) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of mouse PBMC cells using anti-CD14 antibody (A00137).
Overlay histogram showing mouse PBMC cells stained with A00137 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD14 Antibody (A00137,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CD14 (Source: Uniprot.org, NCBI)
Gene Name
CD14
Full Name
Monocyte differentiation antigen CD14
Weight
40.076kDa
Alternative Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein CD14 CD14 molecule monocyte differentiation antigen CD14|myeloid cell-specific leucine-rich glycoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD14, check out the CD14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD14 Antibody Picoband™ (A00137)
Hello CJ!
A00137 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou Z,Liu F,Wang L,Zhu B,Chen Y,Yu Y,Wang X.Inflammation has synergistic effect with nicotine in periodontitis by up-regulating the expression of α7 nAChR via phosphorylated GSK-3β.J Cell Mol Med.2020 Feb;24(4):2663-2676.doi:10.1111/jcmm.14986.Epub 2020 Jan 13.PMID:31930698;PMCID:PMC7028870.
Species: Human,Mouse
A00137 usage in article: APP:FLOW CYTOMETRY, SAMPLE:PDLSCS, DILUTION:NA
Shi M,Shen K,Yang B,Zhang P,Lv K,Qi H,Wang Y,Li M,Yuan Q,Zhang Y. An electroporation strategy to synthesize the membrane-coated nanoparticles for enhanced anti-inflammation therapy in bone infection. Theranostics. 2021 Jan 1;11(5):2349-2363.doi:10.7150/th
Species: Mouse
A00137 usage in article: APP:WB, SAMPLE:RAW CELL, DILUTION:NA
Detection of Vulnerable Atherosclerotic Plaque and Prediction of Thrombosis Events in a Rabbit Model Using18F-FDG -PET/CT
ANTI-INFLAMMATORY ACTIVITY OF PLATYCODIN D ON ALCOHOL-INDUCED FATTY LIVER RATS VIA TLR4-MYD88-NF-?B SIGNAL PATH
Chinese medicinal formula, Qinggan Huoxue Recipe protects rats from alcoholic liver disease via the lipopolysaccharide-Kupffer cell signal conduction pathway
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-CD14 Antibody Picoband™
Question
We are currently using anti-CD14 antibody A00137 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-CD14 antibody (A00137) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
Is a blocking peptide available for product anti-CD14 antibody (A00137)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-CD14 antibody (A00137). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
I see that the anti-CD14 antibody A00137 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-13
Question
We have been able to see staining in mouse lymphocyte. Any tips? Is anti-CD14 antibody supposed to stain lymphocyte positively?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
According to literature lymphocyte does express CD14. According to Uniprot.org, CD14 is expressed in liver, lymphocyte, macrophage, promyelocytic leukemia, brain, glioblastoma, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have CD14 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Glioblastoma, Pubmed ID: 2779588
Liver, Pubmed ID: 19159218, 24275569
Lymphocyte, Pubmed ID: 2453848
Macrophage, Pubmed ID: 2472171
Plasma, Pubmed ID: 16335952
Promyelocytic leukemia, Pubmed ID: 18810425
Boster Scientific Support
Answered: 2019-07-16
Question
Does A00137 anti-CD14 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
You can see on the product datasheet, A00137 anti-CD14 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-09
Question
My question regarding product A00137, anti-CD14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00137 anti-CD14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-27
Question
Is there a BSA free version of anti-CD14 antibody A00137 available?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD14 antibody A00137 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-07
Question
Is this A00137 anti-CD14 antibody reactive to the isotypes of CD14?
J. Jones
Verified customer
Asked: 2019-04-05
Answer
The immunogen of A00137 anti-CD14 antibody is E. coli-derived mouse CD14 recombinant protein (Position: S16-A260). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-05
Question
Does anti-CD14 antibody A00137 work for WB with glioblastoma?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
According to the expression profile of glioblastoma, CD14 is highly expressed in glioblastoma. So, it is likely that anti-CD14 antibody A00137 will work for WB with glioblastoma.
Boster Scientific Support
Answered: 2018-12-14
Question
Please see the WB image, lot number and protocol we used for glioblastoma using anti-CD14 antibody A00137. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-30
Question
We want to test anti-CD14 antibody A00137 on rat glioblastoma for research purposes, then I may be interested in using anti-CD14 antibody A00137 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Miller
Verified customer
Asked: 2018-05-14
Answer
The products we sell, including anti-CD14 antibody A00137, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-14
Question
Our lab used your anti-CD14 antibody for WB on cerebrospinal fluid in the past. I am using rat, and We intend to use the antibody for ELISA next. you antibody examining cerebrospinal fluid as well as glioblastoma in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
I have checked the website and datasheets of our anti-CD14 antibody and it seems that A00137 has been validated on rat in both WB and ELISA. Thus A00137 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-09
Question
Our team were well pleased with the WB result of your anti-CD14 antibody. However we have been able to see positive staining in glioblastoma cell membrane using this antibody. Is that expected? Could you tell me where is CD14 supposed to be expressed?
S. Patel
Verified customer
Asked: 2017-10-24
Answer
According to literature, glioblastoma does express CD14. Generally CD14 expresses in cell membrane. Regarding which tissues have CD14 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Glioblastoma, Pubmed ID: 2779588
Liver, Pubmed ID: 19159218, 24275569
Lymphocyte, Pubmed ID: 2453848
Macrophage, Pubmed ID: 2472171
Plasma, Pubmed ID: 16335952
Promyelocytic leukemia, Pubmed ID: 18810425
Boster Scientific Support
Answered: 2017-10-24
Question
I was wanting to use your anti-CD14 antibody for WB for rat glioblastoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat glioblastoma identification?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
It shows on the product datasheet, A00137 anti-CD14 antibody has been tested for ELISA, Flow Cytometry, IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat glioblastoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for glioblastoma using anti-CD14 antibody A00137. Let me know if you need anything else.
M. Walker
Verified customer
Asked: 2014-11-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-24
