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Pack Size:100μg/vial
Validated Species:Human
Clone Number:CA-4
Application:Flow Cytometry, IHC, ICC
Data & Images


Product Name Anti-CD4 Antibody (Monoclonal, CA-4)
SKU/Catalog Number MA1016
Description Mouse IgG monoclonal antibody for CD4, CD4 molecule (CD4) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.
Cite This Product Anti-CD4 Antibody (Monoclonal, CA-4) CA-4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1016)
Replacement Item This antibody may replace the following items: sc-19643|sc-13573|sc-59041|sc-53664|sc-52385|sc-19641|sc-51547|sc-51545|sc-7219|sc-51544|sc-1140|sc-59034|sc-32811|sc-70659|sc-70670|sc-70674|sc-59032|sc-1176|sc-59031|sc-70660|sc-59042|sc-53838|sc-20079|sc-53042|sc-53041|sc-19642|sc-514746|sc-514571 from Santa Cruz Biotechnology.
Host Mouse
Validated Species Human
Application Flow Cytometry, IHC, ICC

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Monoclonal
Clone Number CA-4
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cd4
Protein Name T-cell surface glycoprotein CD4
Molecular Weight 51438 MW
Protein Function Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
Sequence Similarities Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P05540
Alternative Names T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;W3/25 antigen;CD4;Cd4;
Research Areas |immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|lymphoid|t lymphocytic lineage|myeloid|dendritic cell lineage|monocytic lineage| immunology|regulatory t cells|immune system diseases|antiviral signaling|hiv-related|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for T-cell surface glycoprotein CD4

CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

Anti-CD4 Antibody (Monoclonal, CA-4) Images

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Anti-CD4 Antibody (Monoclonal, CA-4)
Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (MA1016).
Overlay histogram showing human PBMC cells stained with MA1016 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (MA1016,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Customer Q&As

Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applications
A: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.