Anti-CD4 Antibody (Monoclonal, CA-4)
|Ig Isotype||Mouse IgG1|
|Applications||Flow Cytometry, IHC, ICC|
|Product Name||Anti-CD4 Antibody (Monoclonal, CA-4)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CD4, CD4 molecule (CD4) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CD4 Antibody (Monoclonal, CA-4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1016)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
Images And Assay Conditions
Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (MA1016).
Overlay histogram showing human PBMC cells stained with MA1016 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (MA1016,1Î¼g/1x106 cells) for 30 min at 20Â°C. DyLightÂ®488 conjugated goat anti-mouse IgG (BA1126, 5-10Î¼g/1x106 cells) was used as secondary antibody for 30 minutes at 20Â°C. Isotype control antibody (Green line) was mouse IgG (1Î¼g/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info (Source: Uniprot.org)
|Protein Name||T-cell surface glycoprotein CD4|
|Alternative Names||T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;W3/25 antigen;CD4;Cd4;|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Molecular Weight||51438 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.|
*You can search these to find other products in these research areas.
|Background||CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.|
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Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applicationsA: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to email@example.com.