|Application:||Flow Cytometry, IHC, ICC|
Data & Images
|Product Name||Anti-CD4 Antibody (Monoclonal, CA-4)|
|Description||Mouse IgG monoclonal antibody for CD4, CD4 molecule (CD4) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CD4 Antibody (Monoclonal, CA-4) CA-4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1016)|
|Replacement Item||This antibody may replace the following items: sc-19643|sc-13573|sc-59041|sc-53664|sc-52385|sc-19641|sc-51547|sc-51545|sc-7219|sc-51544|sc-1140|sc-59034|sc-32811|sc-70659|sc-70670|sc-70674|sc-59032|sc-1176|sc-59031|sc-70660|sc-59042|sc-53838|sc-20079|sc-53042|sc-53041|sc-19642|sc-514746|sc-514571 from Santa Cruz Biotechnology.|
|Application||Flow Cytometry, IHC, ICC
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||T-cell surface glycoprotein CD4|
|Molecular Weight||51438 MW|
|Protein Function||Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.|
|Sequence Similarities||Contains 3 Ig-like C2-type (immunoglobulin-like) domains.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;W3/25 antigen;CD4;Cd4;|
|Research Areas|||immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|lymphoid|t lymphocytic lineage|myeloid|dendritic cell lineage|monocytic lineage| immunology|regulatory t cells|immune system diseases|antiviral signaling|hiv-related||
Background for T-cell surface glycoprotein CD4
Anti-CD4 Antibody (Monoclonal, CA-4) Images
Click the images to enlarge.
Overlay histogram showing human PBMC cells stained with MA1016 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (MA1016,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.