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Product Info Summary
|Application:||Flow Cytometry, IHC, ICC|
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Anti-CD4 Antibody (Monoclonal, CA-4)
Boster Bio Anti-CD4 Antibody (Monoclonal, CA-4) catalog # MA1016. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD4 Antibody (Monoclonal, CA-4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1016)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
MA1016 is reactive to Cd4 in Human
MA1016 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee
Background of Cd4
CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (MA1016).
Overlay histogram showing human PBMC cells stained with MA1016 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (MA1016,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Gene/Protein Information For Cd4 (Source: Uniprot.Org, NCBI)
T-cell surface glycoprotein CD4
CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 Cd4|W3/25, p55|Cd4 molecule|T-cell surface glycoprotein CD4|CD4 antigen|T-cell surface antigen T4/Leu-3|W3/25 antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cd4, check out the Cd4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
MA1016 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.
Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X. Int J Nanomedicine. 2015 Jan 6;10:347-55. Doi: 10.2147/Ijn.S71245. Ecollection 2015. Treating Cutaneous Squamous Cell Carcinoma Using 5-Aminolevulinic Acid Pol...
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W. Nephron Exp Nephrol. 2014;128(1-2):46-56. Doi: 10.1159/000366446. Epub 2014 Nov 5. Increased Macrophage Activation Inhibited By Tacrolimus In The Kidney Of Diabetic Rats.
Chen X, Wan J, Liu J, Xie W, Diao X, Xu J, Zhu B, Chen Z. Lung Cancer. 2010 Sep;69(3):348-54. Doi: 10.1016/J.Lungcan.2009.11.013. Increased Il-17-Producing Cells Correlate With Poor Survival And Lymphangiogenesis In Nsclc Patients.
Ouyang W, An T, Guo D, Wu S, Tien P. Aids Res Hum Retroviruses. 2013 Mar;29(3):613-20. Doi: 10.1089/Aid.2012.0059. Epub 2013 Jan 11. The Potent Human Immunodeficiency Virus Type 1 (Hiv-1) Entry Inhibitor Hr212 Blocks Formation Of The Envelope Glyc...
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