Anti-CD4 Antibody (Monoclonal, CA-4)
Mouse IgG monoclonal antibody for CD4, CD4 molecule (CD4) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins. Cited in 7 publication(s).
Anti-CD4 Antibody (Monoclonal, CA-4) Info At A Glance
|Applications:||Flow Cytometry, IHC, ICC|
|Product Name||Anti-CD4 Antibody (Monoclonal, CA-4)
See all Cd4 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for CD4 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-F. Reactive species: Human. CD4 information: Molecular Weight: 51438 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.|
|Cite This Product||Anti-CD4 Antibody (Monoclonal, CA-4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1016)|
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|Anti-CD4 Antibody (Monoclonal, CA-4) may replace the following items: sc 19643|sc 13573|sc 59041|sc 53664|sc 52385|sc 19641|sc 51547|sc 51545|sc 7219|sc 51544|sc 1140|sc 59034|sc 32811|sc 70659|sc 70670|sc 70674|sc 59032|sc 1176|sc 59031|sc 70660|sc 59042|sc 53838|sc 20079|sc 53042|sc 53041|sc 19642|sc 514746|sc 514571.|
|Applications||Flow Cytometry, IHC, ICC
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Cd4 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||24932|
|Species Of This Entry||Rat|
|Protein Name||T-cell surface glycoprotein CD4|
|Alternative Names||Cd4|CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Glycosylation; Methylation; Acetylation; Demethylation; Reduction; Dephosphorylation; Ubiquitination; Deglycosylation; Biotinylation; Oxidation; Myristoylation; Ribosylation; Nitration; Deamidation; Deacetylation; Citrullination; Palmitoylation; Phosphorothioation|
|Gene Location||4q42, on Chromasome 4, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Cd4 (Source: Uniprot.org, NCBI)
|Protein Function||Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T- helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.|
|Research Areas||Adaptive Immunity; Cell Biology; Cellular Markers; Cytokine Research; Immunology; Stem Cell Markers|
|Related Diseases||Hiv Infections; Immunologic Deficiency Syndromes; Acquired Immunodeficiency Syndrome; Neoplasms; Autoimmune Reaction; Infective Disorder; Inflammation; Malignant Neoplasms; Autoimmune Diseases; Virus Diseases; Decreased Immunologic Activity [pe]; Aids-related Opportunistic Infections; Tuberculosis; Lymphoma; Hepatitis; Leukemia; Autoimmunity; Pneumonia|
|Related Pathways||Immune Response; Pathogenesis; Secretion; Cell Activation; Cytokine Production; Cell Proliferation; T Cell Activation; Cell Death; Viral Replication; T Cell Proliferation; Hypersensitivity; Cell Development; Cell Differentiation; Localization; Inflammatory Response; Cytokine Secretion; Tropism; Lymphocyte Proliferation; Drug Resistance; Aging|
|Background||CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.|
|Scientific References||PMID: 3104900 by Clark S.J., et al. Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: evidence for derivation from a structure with four immunoglobulin-related domains.|
PMID: 2113054 by Davis S.J., et al. High level expression in Chinese hamster ovary cells of soluble forms of CD4 T lymphocyte glycoprotein including glycosylation variants.
Our Boster Quality Guarantee for Anti-CD4 Antibody (Monoclonal, CA-4) covers its use in the following applications.Immunohistochemistry(Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Overlay histogram showing human PBMC cells stained with MA1016 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (MA1016,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 7
|Ouyang W, An T, Guo D, Wu S, Tien P. Aids Res Hum Retroviruses. 2013 Mar;29(3):613-20. Doi: 10.1089/Aid.2012.0059. Epub 2013 Jan 11. The Potent Human Immunodeficiency Virus Type 1 (Hiv-1) Entry Inhibitor Hr212 Blocks Formation Of The Envelope Glyc...||PubMed ID 23308369|
|Chen X, Wan J, Liu J, Xie W, Diao X, Xu J, Zhu B, Chen Z. Lung Cancer. 2010 Sep;69(3):348-54. Doi: 10.1016/J.Lungcan.2009.11.013. Increased Il-17-Producing Cells Correlate With Poor Survival And Lymphangiogenesis In Nsclc Patients.||PubMed ID 20022135|
|Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W. Nephron Exp Nephrol. 2014;128(1-2):46-56. Doi: 10.1159/000366446. Epub 2014 Nov 5. Increased Macrophage Activation Inhibited By Tacrolimus In The Kidney Of Diabetic Rats.||PubMed ID 25376933|
|Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...||PubMed ID 25816072|
|Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X. Int J Nanomedicine. 2015 Jan 6;10:347-55. Doi: 10.2147/Ijn.S71245. Ecollection 2015. Treating Cutaneous Squamous Cell Carcinoma Using 5-Aminolevulinic Acid Pol...||PubMed ID 25609949|
|Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.||PubMed ID 24038990|
|Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients||PubMed ID 15133849|
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