Human PROC/Protein C ELISA Kit PicoKine®

Coagulation Factor XIV/Protein C ELISA kit for Human

Human PROC/Protein C ELISA Kit PicoKine™ (96 Tests). Quantitate Human Proc in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1ng/ml.

Product Info Summary

SKU: EK1975
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

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Product Name

Human PROC/Protein C ELISA Kit PicoKine®

View all Coagulation Factor XIV/Protein C ELISA kits

SKU/Catalog Number

EK1975

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PROC/Protein C ELISA Kit PicoKine™ (96 Tests). Quantitate Human Proc in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PROC/Protein C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1975)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A43-P461

Sensitivity

<0.1 ng/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1975 is reactive to Proc in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Application Guarantee

EK1975 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Coagulation Factor XIV/Protein C

Protein C (PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped to 2q14.3. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombinthrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

Kit Components

Catalog Number Description Quantity
EK1975-CAP Anti-Human Proc Pre-coated 96-well strip microplate 1
EK1975-ST Human Proc Standard 2 vials, 50 ng/tube
EK1975-DA Human Proc Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0170.1350.2330.4450.8571.3431.8132.017

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Coagulation Factor XIV/Protein C, Dilution ratio of 1:200, concentration in serum is around 2.5ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PROC/Protein C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12235529252311347653925258
Standard deviation52.59 359.391185.8683.51320.411793.32
CV (%)4.3%6.5%4.7%6.2%4.9%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Coagulation Factor XIV/Protein C in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11223117713471353127576.776.0%
Sample 255295748653965446090458.097.5%
Sample 325231232582279425529242031193.034.9%
*number of samples for each test n=16.

Gene/Protein Information For Proc (Source: Uniprot.Org, NCBI)

Uniprot ID

P33587

Gene ID

19123

Gene Name

Proc

Full Name

Vitamin K-dependent protein C

Weight

51.818kDa

Superfamily

peptidase S1 family

Alternative Names

Anticoagulant protein C; APC; Autoprothrombin IIA; Blood coagulation factor XIV; Coagulation Factor XIV; EC 3.4.21; EC 3.4.21.69; PC; PROC; PROC1; protein C (inactivator of coagulation factors Va and VIIIa); Protein C; vitamin K-dependent protein C Proc|P, PC|protein C|vitamin K-dependent protein C|anticoagulant protein C|autoprothrombin IIA|blood coagulation factor XIV|inactivator of coagulation factors Va, VIII

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Proc, check out the Proc Infographic

Proc infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Proc: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human PROC/Protein C ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-02-24

Question

Q: how to proceed with the analysis of ELISA data? I have performed Protein C level in serum.

Verified Customer

Verified customer

Asked: 2020-01-26

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-01-26

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-07-13

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-07-13

Question

Q: if the enzyme conjugated Protein C antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Protein C antigen?

D. Mitchell

Verified customer

Asked: 2019-05-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-05-17

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

D. Hill

Verified customer

Asked: 2018-06-23

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-06-23

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Protein C using the EK1975 Human Protein C Picokine® ELISA Kit?

K. Robinson

Verified customer

Asked: 2017-01-02

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2017-01-02

Question

Q: Can Protein C ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

M. Wang

Verified customer

Asked: 2016-12-28

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-12-28

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