|Applications||ELISA, IHC, WB|
|Product Name||Anti-IL10 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Interleukin-10(IL10) detection. Tested with WB, IHC-P, ELISA in Rat.|
|Cite This Product||Anti-IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1016)|
|Immunogen||E. coli-derived rat IL-10 recombinant protein(Position: S19-N178).|
Assay Dilutions Overview
ELISA , 0.1-0.5μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-rat IL-10 antibody, RP1016, Western blotting
Lane 1: Recombinant Rat IL-10 Protein 10ng
Lane 2: Recombinant Rat IL-10 Protein 5ng
Lane 3: Recombinant Rat IL-10 Protein 2
Protein Target Info (Source: Uniprot.org)
|Alternative Names||Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;Il10;Il-10;|
|Molecular Weight||20426 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. .|
|Background||Interleukin-10(IL-10 or IL10), also known as human cytokine synthesis inhibitory factor(CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al.(1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al.(1997) mapped the IL10 gene to the junction between 1q31 and 1q32.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,