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Product Info Summary
|Application:||ELISA, IHC, WB|
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Anti-Interleukin-10 IL10 Antibody
Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1016. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-10 IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1016)
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
E. coli-derived rat IL-10 recombinant protein (Position: S19-N178).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
RP1016 is reactive to Il10 in Rat
RP1016 is guaranteed for ELISA, IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA , 0.1-0.5μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (RP1016).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # RP1016) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 21KD. The expected band size for IL10 is at 21KD.
Gene/Protein Information For Il10 (Source: Uniprot.Org, NCBI)
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF Il10|IL10X, If2a|interleukin 10|interleukin-10|CSIF|IL-10|cytokine synthesis inhibitory factor|interferon 2a*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il10, check out the Il10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
RP1016 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface
Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats
Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice
Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A
Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy
Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model
Tian Y, Li Y, Li J, Feng S, Li S, Mao J, Xie Y, Liu X, Dong H, Zheng W, Wang M. Evid Based Complement Alternat Med. 2017;2017:1768243. doi: 10.1155/2017/1768243. Epub 2017 Oct 30. Bufei Yishen Granules Combined with Acupoint Sticking Therapy Suppr...
Zhao B, Gao WW, Liu YJ, Jiang M, Liu L, Yuan Q, Hou JB, Xia ZY. Neural Regen Res. 2017 Oct;12(10):1632-1639. doi: 10.4103/1673-5374.217337. The role of glycogen synthase kinase 3 beta in brain injury induced by myocardial ischemia/reperfusion inju...
Gao, W., Zhao, B., Liu, L., Yuan, Q., Wu, X., & Xia, Z. (2017). Myocardial ischemic post-conditioning protects the lung against myocardial ischemia/reperfusion-induced damage by activating GSK-3β. Acta Cirurgica Brasileira, 32(5), 376-387. doi: 10...
Wang Zh, Zeng Xy, Han Sp, Fan Gx, Wang Jy. Neurochem Res. 2012 Aug;37(8):1811-9. Doi: 10.1007/S11064-012-0795-0. Epub 2012 May 15. Interleukin-10 Of Red Nucleus Plays Anti-Allodynia Effect In Neuropathic Pain Rats With Spared Nerve Injury.
Shao Q, Li Y, Wang Q, Zhao J. Neurochem Res. 2015 Apr;40(4):733-9. Doi: 10.1007/S11064-015-1521-5. Epub 2015 Jan 24. Il-10 And Il-1?? Mediate Neuropathic-Pain Like Behavior In The Ventrolateral Orbital Cortex.
Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H. J Exp Clin Cancer Res. 2011 May 20;30:62. Doi: 10.1186/1756-9966-30-62. Increased Il-10 Mrna Expression In Tumor-Associated Macrophage Correlated With Late Stage Of Lung Cancer.
Jin Hb, Yang Yb, Song Yl, Zhang Yc, Li Yr. Mol Biol Rep. 2012 Dec;39(12):11005-9. Doi: 10.1007/S11033-012-2002-4. Epub 2012 Oct 8. Protective Roles Of Quercetin In Acute Myocardial Ischemia And Reperfusion Injury In Rats.
Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.
Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...
Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...
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