Click image to see more details
Product Info Summary
| SKU: | RP1016 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Interleukin-10 IL10 Antibody Picoband®
SKU/Catalog Number
RP1016
Size
100 μg/vial
Form
Lyophilized
Description
IL10 is an immunoregulatory cytokine typically studied in anti-inflammatory signaling and immune homeostasis (putative here due to page access failure), often contrasted with pro-inflammatory cytokine programs in tissues. Assay context: validated for ELISA/IHC/Western blot in Rat; it is commonly interpreted alongside pro-inflammatory localization such as IL1A (putative counter-axis) and cytotoxic effector readouts such as GZMB (granzyme B) (putative cross-context), with quantitative confirmation sometimes performed via ELISA testing (method-context).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-10 IL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1016)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IL-10 recombinant protein (Position: S19-N178).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1016 is reactive to Il10 in Rat
Observed Molecular Weight
17 kDa
Calculated molecular weight
20.4 kDa
Background of Il10
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1016 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
IHC: rat spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IL10 using anti-IL10 antibody (RP1016).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat IL10 protein 10 ng,
Lane 2: recombinant rat IL10 protein 5 ng,
Lane 3: recombinant rat IL10 protein 1 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # RP1016) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa.
Click image to see more details
IHC analysis of IL10 using anti-IL10 antibody (RP1016).
IL10 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL10 Antibody (RP1016) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Interleukin-10 IL10 Antibody Picoband® (RP1016)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Interleukin-10 IL10 Antibody Picoband®
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-IL10 antibody RP1016. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-13
Question
Our lab used your anti-IL10 antibody for ELISA on esophagus in the past. I am using rat, and I plan to use the antibody for WB next. I would like examining esophagus as well as t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
I looked at the website and datasheets of our anti-IL10 antibody and it appears that RP1016 has been tested on rat in both ELISA and WB. Thus RP1016 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-12
Question
Will anti-IL10 antibody RP1016 work for ELISA with t-cell?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
According to the expression profile of t-cell, IL10 is highly expressed in t-cell. So, it is likely that anti-IL10 antibody RP1016 will work for ELISA with t-cell.
Boster Scientific Support
Answered: 2020-02-11
Question
We are interested in to test anti-IL10 antibody RP1016 on rat t-cell for research purposes, then I may be interested in using anti-IL10 antibody RP1016 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The products we sell, including anti-IL10 antibody RP1016, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-28
Question
We need using your anti-IL10 antibody for defense response to bacterium studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
I appreciate your inquiry. This RP1016 anti-IL10 antibody is tested on rat spleen tissue. It is guaranteed to work for ELISA, IHC, WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-14
Question
Does RP1016 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
It shows on the product datasheet, RP1016 anti-IL10 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-07
Question
Do you have a BSA free version of anti-IL10 antibody RP1016 available?
K. Williams
Verified customer
Asked: 2018-09-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody RP1016 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-12
Question
I see that the anti-IL10 antibody RP1016 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-29
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-08-29
Question
I was wanting to use your anti-IL10 antibody for ELISA for rat t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat t-cell identification?
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
It shows on the product datasheet, RP1016 anti-IL10 antibody has been validated for ELISA, IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-07
Question
Is this RP1016 anti-IL10 antibody reactive to the isotypes of IL10?
A. Parker
Verified customer
Asked: 2018-01-29
Answer
The immunogen of RP1016 anti-IL10 antibody is E. coli-derived rat IL-10 recombinant protein(Position: S19-N178). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-29
Question
I have a question about product RP1016, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Roberts
Verified customer
Asked: 2018-01-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1016 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-05
Question
We have observed staining in rat t-cell. Are there any suggestions? Is anti-IL10 antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
From what I have seen in literature t-cell does express IL10. From what I have seen in Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2017-10-27
Question
We are currently using anti-IL10 antibody RP1016 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on bovine tissues as well?
V. Patel
Verified customer
Asked: 2017-05-08
Answer
The anti-IL10 antibody (RP1016) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-08
Question
Our team were satisfied with the WB result of your anti-IL10 antibody. However we have been able to see positive staining in t-cell secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?
M. Krishna
Verified customer
Asked: 2016-12-28
Answer
From literature, t-cell does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2016-12-28
Question
Is a blocking peptide available for product anti-IL10 antibody (RP1016)?
C. Taylor
Verified customer
Asked: 2016-06-10
Answer
We do provide the blocking peptide for product anti-IL10 antibody (RP1016). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-06-10
Question
See attached the WB image, lot number and protocol we used for t-cell using anti-IL10 antibody RP1016. Please let me know if you require anything else.
F. Singh
Verified customer
Asked: 2014-04-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-04
