Anti-Interleukin-10 IL10 Antibody
Rabbit IgG polyclonal antibody for Interleukin-10(IL10) detection. Tested with WB, IHC-P, ELISA in Rat. Cited in 17 publication(s).
Anti-Interleukin-10 IL10 Antibody Info At A Glance
|Applications:||ELISA, IHC, WB|
|Product Name||Anti-Interleukin-10 IL10 Antibody
See all IL-10 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for IL 10/IL10 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Rat. IL 10/IL10 information: Molecular Weight: 20426 MW; Subcellular Localization: Secreted.|
|Cite This Product||Anti-Interleukin-10 IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1016)|
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|Anti-Interleukin-10 IL10 Antibody may replace the following items: sc 323967|sc 323968|sc 32815|sc 53705|sc 57244|sc 7888|sc 81028|sc 8438.|
|Applications||ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||E. coli-derived rat IL-10 recombinant protein(Position: S19-N178).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Il10 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||25325|
|Species Of This Entry||Rat|
|Alternative Names||IL-10|CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF|
|Post Tranlational Modifications||Phosphorylation; Methylation; Acetylation; Cleavage; Oxidation; Demethylation; Glycosylation; Citrullination; Dephosphorylation; Nitration; Nitrosylation; Ubiquitination; Iodination; Reduction; Deacetylation|
|Gene Location||13q13, on Chromasome 13, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Il10 (Source: Uniprot.org, NCBI)
|Protein Function||Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. .|
|Related Diseases||Inflammation; Malignant Neoplasms; Autoimmune Reaction; Inflammatory Response; Neoplasms; Colitis; Autoimmune Diseases; Arthritis; Infective Disorder; Inflammatory Bowel Diseases; Lymphoma; Rheumatoid Arthritis; Intestinal Diseases; Asthma; Sclerosis; Lupus Erythematosus, Systemic; Hepatitis; Diabetes Mellitus; Hiv Infections; Multiple Sclerosis|
|Related Pathways||Immune Response; Secretion; Pathogenesis; Cytokine Production; Inflammatory Response; Cytokine Secretion; Cell Proliferation; Cell Activation; Innate Immune Response; Cell Death; T Cell Proliferation; T Cell Activation; Hypersensitivity; Sensitization; Lymphocyte Proliferation; Cell Cycle; Macrophage Activation; Phagocytosis; Angiogenesis; Methylation|
|Background||Interleukin-10(IL-10 or IL10), also known as human cytokine synthesis inhibitory factor(CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al.(1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al.(1997) mapped the IL10 gene to the junction between 1q31 and 1q32.|
|Scientific References||PMID: 1280414 by Goodman R.E., et al. Synthesis and characterization of rat interleukin-10 (IL-10) cDNA clones from the RNA of cultured OX8-OX22-thoracic duct T cells.|
PMID: 8484757 by Feng L., et al. Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages.
Our Boster Quality Guarantee for Anti-Interleukin-10 IL10 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA , 0.1-0.5μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL10 antigen affinity purified polyclonal antibody (Catalog # RP1016) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 21KD. The expected band size for IL10 is at 21KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 17
|Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...||PubMed ID 25744647|
|Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.||PubMed ID 21246001|
|Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...||PubMed ID 26064167|
|Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.||PubMed ID 25075575|
|Jin Hb, Yang Yb, Song Yl, Zhang Yc, Li Yr. Mol Biol Rep. 2012 Dec;39(12):11005-9. Doi: 10.1007/S11033-012-2002-4. Epub 2012 Oct 8. Protective Roles Of Quercetin In Acute Myocardial Ischemia And Reperfusion Injury In Rats.||PubMed ID 23053990|
|Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H. J Exp Clin Cancer Res. 2011 May 20;30:62. Doi: 10.1186/1756-9966-30-62. Increased Il-10 Mrna Expression In Tumor-Associated Macrophage Correlated With Late Stage Of Lung Cancer.||PubMed ID 21595995|
|Shao Q, Li Y, Wang Q, Zhao J. Neurochem Res. 2015 Apr;40(4):733-9. Doi: 10.1007/S11064-015-1521-5. Epub 2015 Jan 24. Il-10 And Il-1?? Mediate Neuropathic-Pain Like Behavior In The Ventrolateral Orbital Cortex.||PubMed ID 25617163|
|Wang Zh, Zeng Xy, Han Sp, Fan Gx, Wang Jy. Neurochem Res. 2012 Aug;37(8):1811-9. Doi: 10.1007/S11064-012-0795-0. Epub 2012 May 15. Interleukin-10 Of Red Nucleus Plays Anti-Allodynia Effect In Neuropathic Pain Rats With Spared Nerve Injury.||PubMed ID 22584848|
|Gao, W., Zhao, B., Liu, L., Yuan, Q., Wu, X., & Xia, Z. (2017). Myocardial ischemic post-conditioning protects the lung against myocardial ischemia/reperfusion-induced damage by activating GSK-3β. Acta Cirurgica Brasileira, 32(5), 376-387. doi: 10...||PubMed ID 28591367|
|Zhao B, Gao WW, Liu YJ, Jiang M, Liu L, Yuan Q, Hou JB, Xia ZY. Neural Regen Res. 2017 Oct;12(10):1632-1639. doi: 10.4103/1673-5374.217337. The role of glycogen synthase kinase 3 beta in brain injury induced by myocardial ischemia/reperfusion inju...||PubMed ID 29171428|
|Tian Y, Li Y, Li J, Feng S, Li S, Mao J, Xie Y, Liu X, Dong H, Zheng W, Wang M. Evid Based Complement Alternat Med. 2017;2017:1768243. doi: 10.1155/2017/1768243. Epub 2017 Oct 30. Bufei Yishen Granules Combined with Acupoint Sticking Therapy Suppr...||PubMed ID 29234369|
|Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model||PubMed ID 24568666|
|Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy||PubMed ID 27074905|
|Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A||PubMed ID 27347328|
|Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice||PubMed ID 28344826|
|Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats||PubMed ID 26083117|
|Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface||PubMed ID 28902929|
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Note: sample size takes longer to process, average 5-7 days.
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