Anti-Interleukin-10 IL10 Antibody

IL-10 antibody

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1016. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. Cited in 10 publication(s).

Product Info Summary

SKU: RP1016
Size: 100 μg/vial
Reactive Species: Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Interleukin-10 IL10 Antibody

View all IL-10 Antibodies

SKU/Catalog Number

RP1016

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1016. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-10 IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1016)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat IL-10 recombinant protein (Position: S19-N178).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1016 is reactive to IL10 in Rat

Applications

RP1016 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

21 kDa

Calculated molecular weight

20.517kDa

Background of IL-10

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA , 0.1-0.5μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For IL10 (Source: Uniprot.org, NCBI)

Gene Name

IL10

Full Name

Interleukin-10

Weight

20.517kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL10, check out the IL10 Infographic

IL10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

RP1016 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Nuclear factor kappa B regulated monocyte chemoattractant protein-1/chemokine CC motif receptor-2 expressing in spinal cord contributes to the maintenance of cancer-induced bone pain in rats:

Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface

Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats

Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

Tian Y, Li Y, Li J, Feng S, Li S, Mao J, Xie Y, Liu X, Dong H, Zheng W, Wang M. Evid Based Complement Alternat Med. 2017;2017:1768243. doi: 10.1155/2017/1768243. Epub 2017 Oct 30. Bufei Yishen Granules Combined with Acupoint Sticking Therapy Suppr...

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16 Customer Q&As for Anti-Interleukin-10 IL10 Antibody

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-IL10 antibody RP1016. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-13

Question

Our lab used your anti-IL10 antibody for ELISA on esophagus in the past. I am using rat, and I plan to use the antibody for WB next. I would like examining esophagus as well as t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

I looked at the website and datasheets of our anti-IL10 antibody and it appears that RP1016 has been tested on rat in both ELISA and WB. Thus RP1016 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-12

Question

Will anti-IL10 antibody RP1016 work for ELISA with t-cell?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

According to the expression profile of t-cell, IL10 is highly expressed in t-cell. So, it is likely that anti-IL10 antibody RP1016 will work for ELISA with t-cell.

Boster Scientific Support

Answered: 2020-02-11

Question

We are interested in to test anti-IL10 antibody RP1016 on rat t-cell for research purposes, then I may be interested in using anti-IL10 antibody RP1016 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

The products we sell, including anti-IL10 antibody RP1016, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-28

Question

We need using your anti-IL10 antibody for defense response to bacterium studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

I appreciate your inquiry. This RP1016 anti-IL10 antibody is tested on rat spleen tissue. It is guaranteed to work for ELISA, IHC, WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-14

Question

Does RP1016 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-07

Answer

It shows on the product datasheet, RP1016 anti-IL10 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-07

Question

Do you have a BSA free version of anti-IL10 antibody RP1016 available?

K. Williams

Verified customer

Asked: 2018-09-12

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody RP1016 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-09-12

Question

I see that the anti-IL10 antibody RP1016 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-08-29

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-08-29

Question

I was wanting to use your anti-IL10 antibody for ELISA for rat t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat t-cell identification?

Verified Customer

Verified customer

Asked: 2018-03-07

Answer

It shows on the product datasheet, RP1016 anti-IL10 antibody has been validated for ELISA, IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-07

Question

Is this RP1016 anti-IL10 antibody reactive to the isotypes of IL10?

A. Parker

Verified customer

Asked: 2018-01-29

Answer

The immunogen of RP1016 anti-IL10 antibody is E. coli-derived rat IL-10 recombinant protein(Position: S19-N178). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-29

Question

I have a question about product RP1016, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Roberts

Verified customer

Asked: 2018-01-05

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1016 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-05

Question

We have observed staining in rat t-cell. Are there any suggestions? Is anti-IL10 antibody supposed to stain t-cell positively?

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

From what I have seen in literature t-cell does express IL10. From what I have seen in Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510

Boster Scientific Support

Answered: 2017-10-27

Question

We are currently using anti-IL10 antibody RP1016 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on bovine tissues as well?

V. Patel

Verified customer

Asked: 2017-05-08

Answer

The anti-IL10 antibody (RP1016) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-08

Question

Our team were satisfied with the WB result of your anti-IL10 antibody. However we have been able to see positive staining in t-cell secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?

M. Krishna

Verified customer

Asked: 2016-12-28

Answer

From literature, t-cell does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510

Boster Scientific Support

Answered: 2016-12-28

Question

Is a blocking peptide available for product anti-IL10 antibody (RP1016)?

C. Taylor

Verified customer

Asked: 2016-06-10

Answer

We do provide the blocking peptide for product anti-IL10 antibody (RP1016). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-06-10

Question

See attached the WB image, lot number and protocol we used for t-cell using anti-IL10 antibody RP1016. Please let me know if you require anything else.

F. Singh

Verified customer

Asked: 2014-04-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-04

Order DetailsPrice
RP1016

100μg

$370
RP1016-10ug

10μg sample (liquid)

$99
RP1016-Biotin

100 μg Biotin conjugated

$570
RP1016-Cy3

100 μg Cy3 conjugated

$570
RP1016-Dylight488

100 μg Dylight488 conjugated

$570
RP1016-Dylight550

100 μg Dylight550 conjugated

$570
RP1016-Dylight594

100 μg Dylight594 conjugated

$570
RP1016-FITC

100 μg FITC conjugated

$570
RP1016-HRP

100 μg HRP conjugated

$570
RP1016-APC

100 μg APC conjugated

$670
RP1016-PE

100 μg PE conjugated

$670
RP1016-carrier-free

Carrier Free

$370

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