Product Info Summary
| SKU: | M00068-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD40 Mouse Monoclonal Antibody [Clone ID: OTI8B8]
SKU/Catalog Number
M00068-1
Size
100 µl
Description
Boster Bio CD40 mouse monoclonal antibody, clone OTI8B8 (formerly 8B8). Catalog# M00068-1. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-CD40 Mouse Monoclonal Antibody [Clone ID: OTI8B8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00068-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI8B8
Isotype
IgG2a
Immunogen
Human recombinant protein fragment corresponding to amino acids 21-166 of human CD40 (NP_001241) produced in E.coli.
Reactive Species
M00068-1 is reactive to CD40 in Human
Calculated molecular weight
30.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00068-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD40 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
Specific Publications For Anti-CD40 Mouse Monoclonal Antibody [Clone ID: OTI8B8] (M00068-1)
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