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Pack Size:100μg/vial
Validated Species:Human, Mouse, Rat
Application:IHC, ICC, WB
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Product Name Anti-CD43 Picoband™ Antibody
SKU/Catalog Number PB9095
Description Rabbit IgG polyclonal antibody for Leukosialin(SPN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Cite This Product Anti-CD43 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9095)
Replacement Item This antibody may replace the following items: sc-14773|sc-14774|sc-20819|sc-21774|sc-32933|sc-373974|sc-376477|sc-398387|sc-514180|sc-51727|sc-6256|sc-69012|sc-69013|sc-69014|sc-7052|sc-7053|sc-70681|sc-70682 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SPN
Protein Name Leukosialin
Molecular Weight 40322 MW
Protein Function One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). .
Tissue Specificity Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P16150
Alternative Names Leukosialin;Galactoglycoprotein;GALGP;Leukocyte sialoglycoprotein;Sialophorin;CD43;SPN;CD43;
Research Areas |cell biology|apoptosis|intracellular|bcl2 family|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Leukosialin

CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

Anti-CD43 Picoband™ Antibody Images

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Anti-CD43 Picoband™ Antibody
Anti-CD43 Picoband antibody, PB9095, Western blotting
All lanes: Anti CD43 (PB9095) at 0.5ug/ml
WB: Recombinant Human CD43 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti-CD43 Picoband™ Antibody
Anti-CD43 Picoband antibody, PB9095, IHC(P)
IHC(P): Mouse Spleen Tissue
Anti-CD43 Picoband™ Antibody
Anti-CD43 Picoband antibody, PB9095, IHC(P)
IHC(P): Rat Spleen Tissue
Anti-CD43 Picoband™ Antibody
Anti-CD43 Picoband antibody, PB9095, IHC(P)
IHC(P): Human Tonsil Tissue
Anti-CD43 Picoband™ Antibody
Anti-CD43 Picoband antibody, PB9095, ICC
ICC: K562 Cell
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.