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Product Info Summary
| SKU: | M00393-4 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti- IFN Gamma Monoclonal Antibody
SKU/Catalog Number
M00393-4
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IFN Gamma Monoclonal Antibody catalog # M00393-4. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IFN Gamma Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00393-4)
Host
Mouse
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFIG-9
Isotype
Mouse IgG1
Immunogen
Recombinant human IFNγ
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00393-4 is reactive to IFNG in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
19.3 kDa
Background of IFNG
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00393-4 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Validation Images & Assay Conditions
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Western blot analysis of IFN Gamma using anti-IFN Gamma antibody (M00393-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human IFN Gamma protein 15ng,
Lane 2: recombinant human IFN Gamma protein 10ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IFN Gamma antigen affinity purified monoclonal antibody (Catalog # M00393-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Specific Publications For Anti- IFN Gamma Monoclonal Antibody (M00393-4)
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