Anti-CD46 Antibody Picoband™ (monoclonal, 9E9)
Mouse IgG monoclonal antibody for CD46 detection. Tested with WB, IHC-P, FCM in Human.
|Applications||Flow Cytometry, IHC-P, WB|
|Product Name||Anti-CD46 Antibody Picoband™ (monoclonal, 9E9)
See all CD46 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CD46 detection. Tested with WB, IHC-P, FCM in Human.|
|Cite This Product||Anti-CD46 Antibody Picoband™ (monoclonal, 9E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00377-2)|
|Specificity||Anti-CD46 Antibody Picoband™ (monoclonal, 9E9) (M00377-2) reacts with Human CD46, in native form and recombinant. Superfamily members of CD46 are not reactive to M00377-2.|
|Contents/Buffer||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500μg/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CD46 (366-392aa YRYLQRRKKKGTYLTDETHREVKFTSL).|
Our Boster Quality Guarantee for Anti-CD46 Antibody Picoband™ (monoclonal, 9E9) covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-CD46 Antibody Picoband™ (monoclonal, 9E9) (M00377-2).Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti- Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Images And Assay Conditions
Figure 1. Western blot analysis of CD46 using anti ZO-1 antibody (M00377-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- CD46 antigen affinity purified polyclonal antibody (Catalog # M00377-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD46 at approximately 50-70KD. The expected band size for CD46 is at 44KD.
Figure 2. IHC analysis of CD46 using anti- CD46 antibody (M00377-2).
DCK was detected in paraffin-embedded section of human rectum cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- CD46 Antibody (M00377-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis of CD46 using anti- CD46 antibody (M00377-2).
DCK was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD46 Antibody (M00377-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. Flow Cytometry analysis of PBMC cells using anti- CD46 antibody M00377-2).
Overlay histogram showing PBMC cells stained with M00377-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD46 Antibody (M00377-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info (Source: Uniprot.org)
|Protein Name||CD46 molecule, complement regulatory protein|
|Tissue Specificity||Expressed by all cells except erythrocytes.|
|Alternative Names||AHUS2 antibody; Antigen defined by monoclonal antibody; TRA 2 10 antibody; Antigen identified by monoclonal antibody; TRA 2 10 antibody; CD46 antibody; CD46 antigen antibody; CD46 antigen complement regulatory protein antibody; CD46 molecule antibody; CD46 molecule complement regulatory protein antibody; Complement membrane cofactor protein antibody; MCP antibody; MCP_HUMAN antibody; Measles virus receptor antibody; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody; Membrane cofactor protein antibody; MGC26544 antibody;MIC10 antibody; TLX antibody; TRA2.10 antibody; Trophoblast leucocyte common antigen antibody; Trophoblast leukocyte common antigen antibody; Trophoblast lymphocyte cross reactive antigen antibody|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Acts as a cofactor for complement factor I,a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10,and therefore are thought to prevent autoimmunity.|
*You can search these to find other products in these research areas.
|Background||CD46 complement regulatory protein also known as CD46 (cluster of differentiation 46) and Membrane Cofactor Protein is a protein which in humans is encoded by the CD46 gene. The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. And the encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.|
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