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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human CPE/Carboxypeptidase E ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CPE ELISA Kit PicoKine™ (96 Tests). Quantitate Human CPE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CPE/Carboxypeptidase E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1887)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L43-F476
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1887 is reactive to CPE in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1887 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carboxypeptidase E/CPE
This gene encodes a member of the M14 family of metallocarboxypeptidases. It is mapped to 4q32.3. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.
|EK1887-CAP||Anti-Human CPE Pre-coated 96-well strip microplate||1|
|EK1887-ST||Human CPE Standard||2 vials, 10 ng/tube|
|EK1887-DA||Human CPE Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CPE PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Carboxypeptidase E/CPE, Dilution ratio of 1:1, concentration in serum is around 1200pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CPE/Carboxypeptidase E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Carboxypeptidase E/CPE in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CPE (Source: Uniprot.Org, NCBI)
peptidase M14 family
Carboxypeptidase E; Carboxypeptidase H; CPE; CPH; CPH-1; EC 3.4.17; EC 22.214.171.124; Enkephalin convertase; Prohormone-processing carboxypeptidase CPE CPH carboxypeptidase E carboxypeptidase E|carbocypeptidase E|carboxypeptidase H|cobalt-stimulated chromaffin granule carboxypeptidase|enkephalin convertase|epididymis secretory sperm binding protein|insulin granule-associated carboxypeptidase|prohormone-processing carboxypeptidase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CPE, check out the CPE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CPE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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