Anti-CD59 glycoprotein CD59 Antibody

CD59 antibody

Boster Bio Anti-CD59 glycoprotein CD59 Antibody catalog # RP1076. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: RP1076
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-CD59 glycoprotein CD59 Antibody

View all CD59 Antibodies

SKU/Catalog Number

RP1076

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD59 glycoprotein CD59 Antibody catalog # RP1076. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD59 glycoprotein CD59 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1076)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CD59 recombinant protein (Position: L26-N102). Human CD59 shares 47.1% amino acid (aa) sequence identity with rat CD59.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1076 is reactive to CD59 in Human

Applications

RP1076 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

14.177kDa

Background of CD59

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. And this protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry, 1-3μg/1x106cells

Validation Images & Assay Conditions

Gene/Protein Information For CD59 (Source: Uniprot.org, NCBI)

Gene Name

CD59

Full Name

CD59 glycoprotein

Weight

14.177kDa

Alternative Names

16.3A5; 1F5 antigen; 1F5; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16; CD59 antigen; CD59 antigen, complement regulatory protein; CD59 glycoprotein; CD59 molecule, complement regulatory protein; CD59; EJ16; EJ30; EJ30, EL32 and G344); EL32; FLJ38134; FLJ92039; G344; HRF20; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MACIF; MAC-inhibitory protein; MAC-IP; MEM43 antigen; MEM43; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane in CD59 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20 CD59 molecule (CD59 blood group) CD59 glycoprotein|1F5 antigen|20 kDa homologous restriction factor|CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 blood group antigen|CD59 molecule, complement regulatory protein|Ly-6-like protein|MEM43 antigen|T cell-activating protein|human leukocyte antigen MIC11|lymphocytic antigen CD59/MEM43|membrane attack complex (MAC) inhibition factor|membrane attack complex inhibition factor|membrane inhibitor of reactive lysis|protectin|surface anitgen recognized by monoclonal antibody 16.3A5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD59, check out the CD59 Infographic

CD59 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD59: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-CD59 glycoprotein CD59 Antibody

Question

We need using your anti-CD59 antibody for copii-mediated vesicle transport studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

We appreciate your inquiry. This RP1076 anti-CD59 antibody is tested on human tonsil tissue, k562 cells. It is guaranteed to work for Flow Cytometry, IHC, ICC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-17

Question

Our lab used your anti-CD59 antibody for ICC on colon in the past. I am using human, and I plan to use the antibody for IHC next. I am looking for examining colon as well as chorionic villus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

I took a look at the website and datasheets of our anti-CD59 antibody and it appears that RP1076 has been tested on human in both ICC and IHC. Thus RP1076 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-24

Question

We have seen staining in human colon. Any tips? Is anti-CD59 antibody supposed to stain colon positively?

Verified Customer

Verified customer

Asked: 2019-02-28

Answer

According to literature colon does express CD59. According to Uniprot.org, CD59 is expressed in chorionic villus, t-cell, blood, colon, milk, liver, urine, among other tissues. Regarding which tissues have CD59 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1383553
Colon, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Milk, Pubmed ID: 18780401
T-cell, Pubmed ID: 2475570
Urine, Pubmed ID: 7520819

Boster Scientific Support

Answered: 2019-02-28

Question

My team were content with the WB result of your anti-CD59 antibody. However we have been able to see positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is CD59 supposed to be expressed?

C. Edwards

Verified customer

Asked: 2013-11-26

Answer

From literature, liver does express CD59. Generally CD59 expresses in cell membrane. Regarding which tissues have CD59 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1383553
Colon, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Milk, Pubmed ID: 18780401
T-cell, Pubmed ID: 2475570
Urine, Pubmed ID: 7520819

Boster Scientific Support

Answered: 2013-11-26

Question

We are currently using anti-CD59 antibody RP1076 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

P. Gonzalez

Verified customer

Asked: 2013-08-12

Answer

The anti-CD59 antibody (RP1076) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-08-12

Order DetailsPrice
RP1076

100μg

$370
RP1076-10ug

10μg sample (liquid)

$99
RP1076-Biotin

100 μg Biotin conjugated

$570
RP1076-Cy3

100 μg Cy3 conjugated

$570
RP1076-Dylight488

100 μg Dylight488 conjugated

$570
RP1076-Dylight550

100 μg Dylight550 conjugated

$570
RP1076-Dylight594

100 μg Dylight594 conjugated

$570
RP1076-FITC

100 μg FITC conjugated

$570
RP1076-HRP

100 μg HRP conjugated

$570
RP1076-APC

100 μg APC conjugated

$670
RP1076-PE

100 μg PE conjugated

$670
RP1076-carrier-free

Carrier Free

$370

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RP1076
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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