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CD63 antibody (PB9250) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IHC analysis of CD63 using anti-CD63 antibody (PB9250).
CD63 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD63 Antibody (PB9250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
CD63 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD63 Antibody (PB9250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-CD63 Antibody Picoband®
CD63 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
Tested Applications
CD63 antibody was positively detected by WB in:
human MDA-MB-453 whole cell, human U87 whole cell, human HL-60 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CD63 antibody was positively detected by IHC in:
human bladder urothelial carcinoma tissue, human spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD63 Antibody Picoband®
View all CD63 Antibodies
View all CD63 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD63 recombinant protein (Position: E97-M238). Human CD63 shares 74% and 73% amino acid (aa) sequence identity with mouse and rat CD63, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30-60 kDa
Calculated Molecular Weight
25.6 kDa
Background of CD63
CD63 antigen is a protein that in humans is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. It is mapped to 12q13.2. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression.
Cite This Product
Anti-CD63 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9250)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
85%
Moderate Homology
Rabbit
82%
Moderate Homology
Sheep
82%
Moderate Homology
Cow
81%
Moderate Homology
Mouse
74%
Moderate Homology
Rat
73%
Moderate Homology
Zebrafish
44%
Low homology
Drosophila
34%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CD63 antibody
Immunohistochemistry (IHC) protocol for CD63 antibody
Publications
Application:
Species:
Exosomes derived from human amniotic mesenchymal stem cells promotes angiogenesis in hUVECs by delivering novel miRNA N-194
Published: 2025-05-06
Journal: MOLECULAR MEDICINE
Application: WB
Impact Factor: 6.40
Species: Human
PMID: 40329179
Mir-574-3p and Mir-125a-5p in Adipose Derived Mesenchymal Stem Cell Exosomes Synergistically Target TGF-β1/Smad2 Signaling Pathway for the Treatment of Androgenic Alopecia
Published: 2025-04-28
Journal: JOURNAL OF INVESTIGATIVE DERMATOLOGY
Application: WB
Impact Factor: 5.70
Species: Human
PMID: 40306482
Liposome fusogenic enzyme-free circuit enables high-fidelity determination of single exosomal RNA
Published: 2023-03-20
Journal: Materials Today Bio
Application: WB
Impact Factor: 8.20
Species: Human
PMID: 37009069
Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2
Published: 2022-10-14
Journal: Journal of Immunology Research
Application: WB
Impact Factor: 4.49
Species: Human
PMID: 36277474
Cancer-Derived Small Extracellular Vesicles PICKER
Published: 2022-08-18
Journal: ANALYTICAL CHEMISTRY
Application: WB
Impact Factor: 8.01
Species: Human
PMID: 35980378
Hsp16.3 of mycobacterium tuberculosis in exosomes as a biomarker of tuberculosis
Published: 2021-04-24
Journal: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
Application: WB
Impact Factor: 3.27
Species: Human
PMID: 33893878
ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes
Published: 2020-11-09
Journal: MEDIATORS OF INFLAMMATION
Application: WB
Impact Factor: 3.76
Species: Human
PMID: 33223956
Cellular Evidence and Source of Exosomes in the Biliary System of the Chinese Soft-Shelled Turtle, Pelodiscus sinensis
Published: 2019-08-27
Journal: Frontiers in Physiology
Application: IHC,WB
Impact Factor: 3.20
Species: Turtle
PMID: 31507456
Elevated Serum Exosomal miR-125b Level as a Potential Marker for Poor Prognosis in Intermediate-Risk Acute Myeloid Leukemia
Published: 2018-10-10
Journal: ACTA HAEMATOLOGICA
Application: WB
Impact Factor: 1.31
Species: Human
PMID: 30304715
Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A
Published: 2018-10-01
Journal: International Journal of Clinical and Experimental Pathology
Application: WB
Impact Factor: 1.40
Species: Human
PMID: 31949563
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PB9250
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 24, 2026