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Product Info Summary
| SKU: | PB9250 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD63 Antibody Picoband®
SKU/Catalog Number
PB9250
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD63 Antibody Picoband® catalog # PB9250. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD63 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9250)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD63 recombinant protein (Position: E97-M238). Human CD63 shares 74% and 73% amino acid (aa) sequence identity with mouse and rat CD63, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9250 is reactive to CD63 in Human
Observed Molecular Weight
30-60 kDa
Calculated molecular weight
25.6 kDa
Background of CD63
CD63 antigen is a protein that in humans is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. It is mapped to 12q13.2. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9250 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human MDA-MB-453 whole cell, human U87 whole cell, human HL-60 whole cell
IHC: human bladder urothelial carcinoma tissue, human spleen tissue
Validation Images & Assay Conditions
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Identification of hAMSC-Exos ( A ) The morphology of hAMSC-Exos was observed using transmission electron microscopy. Scale bar = 100 nm. ( B ) The hAMSC-Exos particle size distribution was detected using NTA. ( C ) Western blot was used to detect the expression of hAMSC-Exos surface proteins CD9, CD63 and CD81
Index in PubMed under a CC BY license. PMID: 40329179
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Western blot analysis of CD63 using anti-CD63 antibody (PB9250).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MDA-MB-453 whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD63 antigen affinity purified polyclonal antibody (Catalog # PB9250) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD63 at approximately 30-60 kDa. The expected band size for CD63 is at 26 kDa.
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IHC analysis of CD63 using anti-CD63 antibody (PB9250).
CD63 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD63 Antibody (PB9250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of CD63 using anti-CD63 antibody (PB9250).
CD63 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD63 Antibody (PB9250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CD63 Antibody Picoband® (PB9250)
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Customer Q&As
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16 Customer Q&As for Anti-CD63 Antibody Picoband®
Question
Can you help my question with product PB9250, anti-CD63 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9250 anti-CD63 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-17
Question
Is there a BSA free version of anti-CD63 antibody PB9250 available?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD63 antibody PB9250 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-CD63 antibody PB9250. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-09
Question
Would anti-CD63 antibody PB9250 work for IHC with skeletal muscle?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
According to the expression profile of skeletal muscle, CD63 is highly expressed in skeletal muscle. So, it is likely that anti-CD63 antibody PB9250 will work for IHC with skeletal muscle.
Boster Scientific Support
Answered: 2019-06-27
Question
We are currently using anti-CD63 antibody PB9250 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The anti-CD63 antibody (PB9250) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-13
Question
I see that the anti-CD63 antibody PB9250 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-12
Question
We have been able to see staining in human platelet. Do you have any suggestions? Is anti-CD63 antibody supposed to stain platelet positively?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Based on literature platelet does express CD63. Based on Uniprot.org, CD63 is expressed in upper lobe of lung, ovary, skin, skeletal muscle, lung muscle, platelet, placenta, liver, among other tissues. Regarding which tissues have CD63 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, and Muscle, Pubmed ID: 15489334
Ovary, Pubmed ID: 2171551
Placenta, Pubmed ID: 17897319
Platelet, Pubmed ID: 7682577
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-13
Question
Is a blocking peptide available for product anti-CD63 antibody (PB9250)?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
We do provide the blocking peptide for product anti-CD63 antibody (PB9250). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-15
Question
My boss were satisfied with the WB result of your anti-CD63 antibody. However we have been able to see positive staining in ovary cell membrane using this antibody. Is that expected? Could you tell me where is CD63 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
From literature, ovary does express CD63. Generally CD63 expresses in cell membrane. Regarding which tissues have CD63 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, and Muscle, Pubmed ID: 15489334
Ovary, Pubmed ID: 2171551
Placenta, Pubmed ID: 17897319
Platelet, Pubmed ID: 7682577
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-12-03
Question
Is this PB9250 anti-CD63 antibody reactive to the isotypes of CD63?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
The immunogen of PB9250 anti-CD63 antibody is E.coli-derived human CD63 recombinant protein (Position: E97-M238). Human CD63 shares 74% and 73% amino acid (aa) sequence identity with mouse and rat CD63, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-02
Question
We are interested in to test anti-CD63 antibody PB9250 on human skeletal muscle for research purposes, then I may be interested in using anti-CD63 antibody PB9250 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Carter
Verified customer
Asked: 2018-04-27
Answer
The products we sell, including anti-CD63 antibody PB9250, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-27
Question
I was wanting to use your anti-CD63 antibody for IHC for human skeletal muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skeletal muscle identification?
K. Jones
Verified customer
Asked: 2018-02-28
Answer
As indicated on the product datasheet, PB9250 anti-CD63 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-28
Question
Will PB9250 anti-CD63 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Jones
Verified customer
Asked: 2017-08-30
Answer
It shows on the product datasheet, PB9250 anti-CD63 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-30
Question
We ordered your anti-CD63 antibody for WB on skeletal muscle a few years ago. I am using human, and We want to use the antibody for IHC next. I am interested in examining skeletal muscle as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
K. Moore
Verified customer
Asked: 2016-05-09
Answer
I took a look at the website and datasheets of our anti-CD63 antibody and it appears that PB9250 has been tested on human in both WB and IHC. Thus PB9250 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-05-09
Question
I am interested in using your anti-CD63 antibody for positive regulation of receptor internalization studies. Has this antibody been tested with western blotting on intestinal cancer tissue? We would like to see some validation images before ordering.
C. Anderson
Verified customer
Asked: 2015-02-19
Answer
We appreciate your inquiry. This PB9250 anti-CD63 antibody is validated on hela whole cell lysate, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-02-19
Question
See below the WB image, lot number and protocol we used for skeletal muscle using anti-CD63 antibody PB9250. Please let me know if you require anything else.
R. Banerjee
Verified customer
Asked: 2013-04-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-29
