Anti-IL33 Antibody Picoband™

Boster Bio Anti-IL33 Antibody Picoband™ catalog # A00113. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A00113
Size: 100ug/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-IL33 Antibody Picoband™

See all IL-33 products

SKU/Catalog Number

A00113

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-IL33 Antibody Picoband™ catalog # A00113. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL33 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00113)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human IL33 recombinant protein (Position: A95-T270).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00113 is reactive to IL33 in Human

Applications

A00113 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For IL33 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8BVZ5

Gene ID

77125

Gene Name

IL33

Full Name

Interleukin-33

Weight

29.991kDa

Superfamily

IL-1 family

Alternative Names

C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2 Il33|9230117N10Rik, Il-, Il-33, Il1f, Il1f11, NF-H, NF-HEV|interleukin 33|interleukin-33|nuclear factor from high endothelial venules

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL33, check out the IL33 Infographic

IL33 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL33: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00113

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4 Customer Q&As for Anti-IL33 Antibody Picoband™

Question

Our team were happy with the WB result of your anti-IL33 antibody. However we have seen positive staining in left coronary artery nucleus using this antibody. Is that expected? Could you tell me where is IL33 supposed to be expressed?

A. Johnson

Verified customer

Asked: 2017-04-07

Answer

From what I have seen in literature, left coronary artery does express IL33. Generally IL33 expresses in nucleus. Regarding which tissues have IL33 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Trachea, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Endothelial cell, Pubmed ID: 12819012

Boster Scientific Support

Answered: 2017-04-07

Question

We are currently using anti-IL33 antibody A00113 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

B. Yang

Verified customer

Asked: 2015-11-23

Answer

The anti-IL33 antibody (A00113) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-11-23

Question

We have tried in the past anti-IL33 antibody for WB on brain in a previous experiment. I am using human, and I plan to use the antibody for ELISA next. I am interested in examining brain as well as endothelial cell in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?

T. Jha

Verified customer

Asked: 2015-04-23

Answer

I have checked the website and datasheets of our anti-IL33 antibody and it appears that A00113 has been validated on human in both WB and ELISA. Thus A00113 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-04-23

Question

We have seen staining in human adrenal gland trachea. What should we do? Is anti-IL33 antibody supposed to stain adrenal gland trachea positively?

C. Zhang

Verified customer

Asked: 2014-09-02

Answer

Based on literature adrenal gland trachea does express IL33. Based on Uniprot.org, IL33 is expressed in left coronary artery, endothelial cell, adrenal gland trachea, brain, among other tissues. Regarding which tissues have IL33 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Trachea, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Endothelial cell, Pubmed ID: 12819012

Boster Scientific Support

Answered: 2014-09-02

Conjugation

how to order through PO

Size

Total: $280

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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A00113
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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