Overview
| Product Name |
Mouse IL-33 ELISA Kit PicoKine™
See all IL-33 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0930 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Mouse IL33 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL33 tissue specificity: |
| Cite This Product |
Mouse IL-33 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0930)
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
31.2pg/ml-2000pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: S109-I266 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For Il33 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q8BVZ5 |
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| NCBI Gene Id | 77125 |
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| Species Of This Entry | Mouse |
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| Gene Name | Il33 |
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| Protein Name | Interleukin-33 |
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| Superfamily | IL-1 family |
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| Alternative Names | IL-33|C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2 |
|---|
| Molecular Weight | 29991 |
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| Post Tranlational Modifications | Cleavage; Phosphorylation; Methylation; Biotinylation; Reduction; Citrullination |
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| Gene Location | 19|19 C1, on Chromasome 19, gene sequence: |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Il33 (Source: Uniprot.org, NCBI)
| Protein Function | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. |
|---|
| Related Diseases | Inflammation; Asthma; Arthritis; Rheumatoid Arthritis; Autoimmune Reaction; Eosinophilia; Ulcer; Atherosclerosis; Fibrosis; Colitis; Rhinorrhea; Ulcerative Colitis; Autoimmune Diseases; Dermatitis; Allergy; Dermatitis, Atopic; Allergic Asthma; Hypertrophy; Myocardial Infarction; Tissue Adhesions |
|---|
| Related Pathways | Pathogenesis; Secretion; Immune Response; Cytokine Production; Inflammatory Response; Angiogenesis; Cell Activation; Localization; Mast Cell Activation; Cell Differentiation; Basophil Activation; Wound Healing; Cell Adhesion; Cell Proliferation; Endothelial Cell Activation; Anaphylaxis; Proteolysis; Hypersensitivity; Protein Secretion; Reverse Transcription |
|---|
| Background | Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2. |
|---|
| Scientific References | PMID: 16286016 by Schmitz J., et al. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST 2 and induces T helper type 2-associated cytokines.
PMID: 19465481 by Talabot-Ayer D., et al. Interleukin-33 is biologically active independently of caspase-1 cleavage. |
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Kit Components And Assay QC Data Details Of Mouse IL-33 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0930-CAP | 96-well plate precoated with anti-Mouse IL-33 antibody | 1 |
| EK0930-ST | lyophilized recombinant Mouse IL-33 standard | 10ng/tube |
| EK0930-DA | biotinylated anti-Mouse IL-33 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0930 Mouse IL-33 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.016 | 0.070 | 0.122 | 0.234 | 0.450 | 0.909 | 1.536 | 2.294 |
We measured random samples of Mouse IL-33 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 63 | 290 | 939 | 67 | 300 | 883 |
| Standard deviation | 4.72 | 22.62 | 44.13 | 7.52 | 27.6 | 50.33 |
| CV(%) | 7.5% | 7.8% | 4.7% | 7.8% | 9.2% | 5.7% |
We measured the performance consistency of Mouse IL-33 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 63 | 56 | 58 | 57 | 58 | 2.69 | 4.6% |
| Sample 2 | 290 | 253 | 299 | 266 | 277 | 18.37 | 6.6% |
| Sample 3 | 939 | 813 | 894 | 950 | 899 | 53.9 | 5.9% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all IL-33 primary antibodies,
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Total number of citations: 1
| Shen, Z., Yang, H., Yang, S., Wang, W., Cui, X., Zhou, X.,..., & Liu, J. (2017). Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance. Nature Communications, 8(1), 2119. doi: 10.1038/s41467-017-02304-7 |
PubMed ID 29242561 |
