SKU | EK0930 |
---|---|
Size | 96wells/kit, with removable strips. |
Reactivity | Mouse |
Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
Sensitivity | <10pg/ml |
Assay Range | 31.2pg/ml-2000pg/ml |
Overview
Product Name | Mouse IL-33 PicoKine™ ELISA Kit |
---|---|
SKU/Catalog Number | EK0930 |
Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Size | 96wells/kit, with removable strips. |
Description | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-33. 96wells/kit, with removable strips. |
Cite This Product | Mouse IL-33 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0930) |
Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility. |
Sensitivity | <10pg/ml |
Assay Range | 31.2pg/ml-2000pg/ml |
Immunogen | Expression system for standard: E.coli; Immunogen sequence: S109-I266 |
Reactivity | Mouse |
Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
Antibody Clonalities | Capture Antibody | Detection Antibody: polyclonal antibody from goat|polyclonal antibody from goat |
Assay Details
Kit Components
Catalog number | Description | Quantity |
EK0930-CAP | 96-well plate precoated with anti-Mouse IL33 antibody | 1 |
EK0930-ST | lyophilized recombinant Mouse IL33 standard | 10ng/tube |
EK0930-DA | biotinylated anti-Mouse IL33 antibody | 130ul |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Mouse IL-33 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-20min)
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.016 | 0.070 | 0.122 | 0.234 | 0.450 | 0.909 | 1.536 | 2.294 |
Intra/Inter Assay Precision
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 63 | 290 | 939 | 67 | 300 | 883 |
Standard deviation | 4.72 | 22.62 | 44.13 | 7.52 | 27.6 | 50.33 |
CV(%) | 7.5% | 7.8% | 4.7% | 7.8% | 9.2% | 5.7% |
Reproducibility
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 63 | 56 | 58 | 57 | 58 | 2.69 | 4.6% |
Sample 2 | 290 | 253 | 299 | 266 | 277 | 18.37 | 6.6% |
Sample 3 | 939 | 813 | 894 | 950 | 899 | 53.9 | 5.9% |
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Target Info
Protein Target Info (Source: Uniprot.org)
Uniprot Id | Q8BVZ5 |
---|---|
Gene Name | IL33 |
Protein Name | Interleukin-33 |
Alternative Names | Interleukin-33;IL-33;Interleukin-33(102-266);Interleukin-33(109-266);Il33; |
Subcellular Localization | Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle . Secreted. Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent (By similarity). . |
Molecular Weight | 29991 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
Protein Function | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. |
---|---|
Research Areas | Angiogenesis, Cardiovascular, Cytokines, Hypertrophy, Immunology, Innate Immunity, Interleukins *You can search these to find other products in these research areas. |
Background | Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2. |
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (EK0930)
Promotion:
Order 4+ of the same ELISA kit and get 20% off| Order 20+ of the same ELISA kit and get 30% off .Troubleshooting
Boster Guarantee

Guaranteed product quality
We promise all of our products perform as described in datasheets.
Publications
Customer Q&As
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
Keywords: component, ingredient
Keywords: expiration, storage, temperature, size