Overview
| Product Name |
Mouse IL-33 ELISA Kit PicoKine™
See more IL-33 products |
| Catalog# |
EK0930 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Mouse IL-33 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il33 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Mouse IL-33 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0930)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
31.2 pg/ml - 2,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: S109-I266 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Il33 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q8BVZ5 |
|---|
| NCBI Gene Id | 77125 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Il33 |
|---|
| Protein Name | Interleukin-33 |
|---|
| Superfamily | IL-1 family |
|---|
| Alternative Names | IL-33|C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2 |
|---|
| Molecular Weight | 29991 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il33, check out the Il33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Il33: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Il33 infographic
Kit Components And Assay QC Data Details Of Mouse IL-33 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0930-CAP |
Anti-Mouse Il33 Pre-coated 96-well strip microplate | 1 |
| EK0930-ST | Mouse Il33 Standard | 2 vials, 10 ng/tube |
| EK0930-DA | Mouse Il33 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0930 Mouse IL-33 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.016 | 0.070 | 0.122 | 0.234 | 0.450 | 0.909 | 1.536 | 2.294 |
We measured random samples of Mouse IL-33 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 63 | 290 | 939 | 67 | 300 | 883 |
| Standard deviation | 4.72 | 22.62 | 44.13 | 7.52 | 27.6 | 50.33 |
| CV (%) | 7.5% | 7.8% | 4.7% | 7.8% | 9.2% | 5.7% |
We measured the performance consistency of Mouse IL-33 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 63 | 56 | 58 | 57 | 58 | 2.69 | 4.6% |
| Sample 2 | 290 | 253 | 299 | 266 | 277 | 18.37 | 6.6% |
| Sample 3 | 939 | 813 | 894 | 950 | 899 | 53.9 | 5.9% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| Shen, Z., Yang, H., Yang, S., Wang, W., Cui, X., Zhou, X.,..., & Liu, J. (2017). Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance. Nature Communications, 8(1), 2119. doi: 10.1038/s41467-017-02304-7 |
PubMed ID 29242561 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0930 Mouse IL-33 ELISA Kit PicoKine™
8 Related Questions
Question
Q: if the enzyme conjugated IL-33 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-33 antigen?
T. Adams
Asked: 2020-11-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-05
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2020-02-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-02-09
Question
Q: Can IL-33 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Asked: 2019-10-22
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-10-22
Question
Q: how to thaw whole blood sample for IL-33 ELISA after freezing?
K. Dayal
Asked: 2019-08-25
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-33 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2019-02-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-22
Question
Q: What is the optimal O.D. value for IL-33 ELISA kit? I used your IL-33 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-33 even though the O.D. values are not very high?
R. Surve
Asked: 2017-02-06
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-33 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-33 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-02-06
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2016-02-02
Boster Scientific Support
Answered: 2016-02-02
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-33 using the EK0930 Mouse IL-33 Picokine® ELISA Kit?
Verified Customer
Asked: 2015-11-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2015-11-13
