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Product Info Summary
| SKU: | M01399 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD79B Monoclonal Antibody
SKU/Catalog Number
M01399
BM5684 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD79B Monoclonal Antibody catalog # M01399. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD79B Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01399)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFBI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD79b
Reactive Species
M01399 is reactive to CD79B in Human, Mouse, Rat
Observed Molecular Weight
30-50 kDa
Calculated molecular weight
26.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01399 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD79b expression in Raji cell lysate.
Specific Publications For Anti-CD79B Monoclonal Antibody (M01399)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD79B Monoclonal Antibody
Question
I was wanting to use your anti-CD79B Monoclonal antibody for IF for mouse lymph on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lymph identification?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
You can see on the product datasheet, M01399 anti-CD79B Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
What is the sample target on Western blot analysis in your product image for M01399?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-CD79B Monoclonal Antibody (M01399) expression in Raji cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
Is there a BSA free version of anti-CD79B Monoclonal antibody M01399 available?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD79B Monoclonal antibody M01399 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-17
Question
Can you help my question with product M01399, anti-CD79B Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01399 anti-CD79B Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-27
Question
I have attached the WB image, lot number and protocol we used for lymph using anti-CD79B Monoclonal antibody M01399. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-19
Question
Is this M01399 anti-CD79B Monoclonal antibody reactive to the isotypes of CD79B?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
The immunogen of M01399 anti-CD79B Monoclonal antibody is A synthesized peptide derived from human CD79b. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-11
Question
you antibody to test anti-CD79B Monoclonal antibody M01399 on mouse lymph for research purposes, then I may be interested in using anti-CD79B Monoclonal antibody M01399 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Carter
Verified customer
Asked: 2019-05-31
Answer
The products we sell, including anti-CD79B Monoclonal antibody M01399, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-CD79B Monoclonal antibody M01399. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-24
Question
Will M01399 anti-CD79B Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Thomas
Verified customer
Asked: 2018-12-12
Answer
As indicated on the product datasheet, M01399 anti-CD79B Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-12
Question
Will anti-CD79B Monoclonal antibody M01399 work for IF with lymph?
C. Jha
Verified customer
Asked: 2018-11-21
Answer
According to the expression profile of lymph, CD79B is highly expressed in lymph. So, it is likely that anti-CD79B Monoclonal antibody M01399 will work for IF with lymph.
Boster Scientific Support
Answered: 2018-11-21
Question
We are currently using anti-CD79B Monoclonal antibody M01399 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
The anti-CD79B Monoclonal antibody (M01399) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-11
Question
We ordered your anti-CD79B Monoclonal antibody for IP on spleen a few years ago. I am using mouse, and We want to use the antibody for WB next. I am interested in examining spleen as well as small intestine spleen in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
I have checked the website and datasheets of our anti-CD79B Monoclonal antibody and it seems that M01399 has been validated on mouse in both IP and WB. Thus M01399 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-13
Question
Is a blocking peptide available for product anti-CD79B Monoclonal antibody (M01399)?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
We do provide the blocking peptide for product anti-CD79B Monoclonal antibody (M01399). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-08-25
Question
We have observed staining in rat lymph. What should we do? Is anti-CD79B Monoclonal antibody supposed to stain lymph positively?
K. Jones
Verified customer
Asked: 2017-04-24
Answer
From what I have seen in literature lymph does express CD79B. From what I have seen in Uniprot.org, CD79B is expressed in spleen, small intestine spleen, lymph, among other tissues. Regarding which tissues have CD79B expression, here are a few articles citing expression in various tissues:
Lymph, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-04-24
Question
Our lab were content with the WB result of your anti-CD79B Monoclonal antibody. However we have observed positive staining in small intestine spleen cell membrane using this antibody. Is that expected? Could you tell me where is CD79B supposed to be expressed?
J. Kulkarni
Verified customer
Asked: 2017-01-19
Answer
According to literature, small intestine spleen does express CD79B. Generally CD79B expresses in cell membrane. Regarding which tissues have CD79B expression, here are a few articles citing expression in various tissues:
Lymph, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-01-19
Question
I see that the anti-CD79B Monoclonal antibody M01399 works with IF, what is the protocol used to produce the result images on the product page?
K. Baker
Verified customer
Asked: 2013-06-10
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-06-10
