This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CD79B antibody (M01399-1) performs well in IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of CD79b using anti-CD79b antibody (M01399-1).
CD79b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD79b Antibody (M01399-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
CD79b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD79b Antibody (M01399-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Bundle Save
Anti-CD79b Antibody Picoband®(monoclonal, 6H11)
CD79B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Mouse | IHC, WB |
Tested Applications
CD79B antibody was positively detected by WB in:
human Raji whole cell,, human Raji whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CD79B antibody was positively detected by IHC in:
human tonsil tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Recommend
Product Information
Product Name
Anti-CD79b Antibody Picoband®(monoclonal, 6H11)
View all CD79B Antibodies
View all CD79B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6H11
Isotype
Mouse IgG1
Immunogen
E.coli-derived human CD79b recombinant protein (Position: A29-E229). Human CD79b shares 70% amino acid (aa) sequence identity with mouse CD79b.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
26.0 kDa
Background of CD79B
CD79b molecule, immunoglobulin-associated beta, also known as CD79B (Cluster of Differentiation 79B), is a human gene. By fluorescence in situ hybridization, It is mapped to 17q23.3. The CD79B protein together with the related CD79A protein, forms a dimer associated with membrane bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR) which is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). CD79b also can enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
Cite This Product
Anti-CD79b Antibody Picoband®(monoclonal, 6H11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01399-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
86%
Moderate Homology
Dog
78%
Moderate Homology
Sheep
73%
Moderate Homology
Rabbit
72%
Moderate Homology
Cow
72%
Moderate Homology
Mouse
70%
Moderate Homology
Rat
69%
Low homology
Zebrafish
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CD79B antibody
Immunohistochemistry (IHC) protocol for CD79B antibody
Review
Have you used Anti-CD79b Antibody Picoband®(monoclonal, 6H11)?
Submit a review and receive an Amazon gift card.
$30 for a review with an image
Submit A ReviewProduct Promise
We promise Anti-CD79b Antibody Picoband®(monoclonal, 6H11) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01399-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 20, 2026