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- Table of Contents
Cd8a antibody (A02236-1) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 20 Publications
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Flow Cytometry analysis of mouse spleen tissues using anti-CD8 alpha antibody (A02236-1).
Overlay histogram showing mouse spleen tissues stained with A02236-1 (Blue line). The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD8 alpha Antibody (A02236-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing mouse spleen tissues stained with A02236-1 (Blue line). The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD8 alpha Antibody (A02236-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CD8 alpha/Cd8a Antibody Picoband®
Cd8a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
Cd8a antibody was positively detected by WB in:
rat spleen tissue, rat thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cd8a antibody was positively detected by IHC in:
mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Cd8a antibody was positively detected by FC in:
mouse spleen tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
FC
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Tested
Product Information
Product Name
Anti-CD8 alpha/Cd8a Antibody Picoband®
View all CD8A Antibodies
View all CD8A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
27.5 kDa
Background of Cd8a
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Cite This Product
Anti-CD8 alpha/Cd8a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02236-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
62%
Low homology
Monkey
49%
Low homology
Human
49%
Low homology
Dog
44%
Low homology
Sheep
43%
Low homology
Rabbit
41%
Low homology
Cow
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cd8a antibody
Immunohistochemistry (IHC) protocol for Cd8a antibody
Flow Cytometry (FC) protocol for Cd8a antibody
Publications
Application:
Species:
Carrier-Free Nanoparticles Enhance Photothermal–Immune Therapy via Metabolic Reprogramming in Triple-Negative Breast Cancer
Published: 2026-07-11
Journal: Advanced Healthcare Materials
Application: FC
Impact Factor: 11.00
Species: Mouse
PMID: 42434841
Resilience and Vulnerabilities of Tumor Cells under Purine Shortage Stress
Published: 2025-10-15
Journal: CLINICAL CANCER RESEARCH
Application: IHC
Impact Factor: 10.20
Species: Mouse
PMID: 40788171
Lung Cancer Cell-intrinsic Asparagine Synthetase Potentiates Anti-Tumor Immunity via Modulating Immunogenicity and Facilitating Immune Remodeling in Metastatic Tumor-draining Lymph Nodes
Published: 2025-10-10
Journal: International Journal of Biological Sciences
Application: mIF
Impact Factor: 10.00
Species: Mouse
PMID: 41208878
LncRNA-CTD suppresses metastasis and immune evasion by modulating snail1 and MHC-I expression in colorectal cancer
Published: 2025-09-15
Journal: Journal for ImmunoTherapy of Cancer
Application: IHC
Impact Factor: 10.60
Species: Mouse
PMID: 40954077
Spatially resolved multi-omics reveals the renal cortex-metabolic reprogramming of Shenhua Tablet for intervention on IgA nephropathy
Published: 2025-04-14
Journal: PHYTOMEDICINE
Application: IHC
Impact Factor: 8.30
Species: Rat
PMID: 40233505
Ultrasmall PtIr Bimetallic Nanozyme Treats Myocardial Infarction via Ischemic/Inflammatory Cardiac Microenvironment Remodeling
Published: 2025-04-02
Journal: ACS Nano
Application: IHC
Impact Factor: 16.00
Species: Rat
PMID: 40175295
Adenosine triphosphate release inhibitors targeting pannexin1 improve recovery after spinal cord injury
Published: 2024-08-01
Journal: Nagoya Journal of Medical Science
Application: IHC
Impact Factor: 0.90
Species: Rat
PMID: 39355370
Electrospun nanofibrous mats loaded with gemcitabine and cisplatin suppress bladder tumor growth by improving the tumor immune microenvironment
Published: 2024-03-25
Journal: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Application: IF,IHC
Impact Factor: 4.20
Species: Mouse
PMID: 38526656
Targeting complement C5a to improve radiotherapy sensitivity in non-small cell lung cancer
Published: 2023-05-29
Journal: Translational Lung Cancer Research
Application: IF
Impact Factor: 4.00
Species: Mouse
PMID: 37323177
Treating cutaneous squamous cell carcinoma using 5-aminolevulinic acid polylactic-co-glycolic acid nanoparticle-mediated photodynamic therapy in a mouse model
Published: 2023-02-27
Journal: International Journal of Nanomedicine
Application: IHC
Impact Factor: 8.00
Species: Mouse
PMID: 25609949
Intravoxel Incoherent Motion Diffusion-Weighted MR Imaging for Monitoring the Immune Response of Immunogenic Chemotherapy
Published: 2022-05-13
Journal: Frontiers in Oncology
Application: IHC
Impact Factor: 5.74
Species: Human,Mouse
PMID: 35646652
Efficient starvation therapy with three-pathway blocking in combination with PTT/CDT for TME reversal and tumor apoptosis
Published: 2022-03-28
Journal: JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
Application: IHC
Impact Factor: 6.76
Species: Mouse
Mitochondrial targeted melanin@mSiO2 yolk-shell nanostructures for NIR-Ⅱ-driven photo-thermal-dynamic/immunotherapy
Published: 2022-01-24
Journal: CHEMICAL ENGINEERING JOURNAL
Application: IF
Impact Factor: 16.74
Species: Mouse
Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation
Published: 2021-11-23
Journal: CELL TRANSPLANTATION
Application: IHC
Impact Factor: 4.06
Species: Rat,Mouse
PMID: 34814739
A NO/ROS/RNS cascaded-releasing nano-platform for gas/PDT/PTT/immunotherapy of tumors
Published: 2021-06-29
Journal: Biomaterials Science
Application: IHC
Impact Factor: 6.84
Species: Mouse
PMID: 34269777
Berberine Prolongs Mouse Heart Allograft Survival by Activating T Cell Apoptosis via the Mitochondrial Pathway
Published: 2021-02-25
Journal: Frontiers in Immunology
Application: IF,IHC
Impact Factor: 7.56
Species: Mouse
PMID: 33732240
USP7 targeting modulates anti-tumor immune response by reprogramming Tumor-associated Macrophages in Lung Cancer
Published: 2020-07-23
Journal: Theranostics
Application: IF
Impact Factor: 8.58
Species: Mouse
PMID: 32802195
Increased Macrophage Activation Inhibited by Tacrolimus in the Kidney of Diabetic Rats
Published: 2014-11-05
Journal: NEPHRON EXPERIMENTAL NEPHROLOGY
Application: IHC
Impact Factor: 1.65
Species: Rat
PMID: 25376933
Interleukin-15 gene therapy and the mammalian target of rapamycin inhibitor everolimus inhibit the growth of metastatic breast cancer
Published: 2013-09-03
Journal: JOURNAL OF GENE MEDICINE
Application: IHC
Impact Factor: 2.16
Species: Mouse
PMID: 24038990
Induction of immune tolerance with heart-thymus composite allotransplantation in rats
Published: 2005-10-01
Journal: JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY
Impact Factor: 0.19
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Product Promise
We promise Anti-CD8 alpha/Cd8a Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A02236-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026