Anti-CD81 antigen CD81 Antibody

Boster Bio Anti-CD81 antigen CD81 Antibody catalog # A01281-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A01281-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-CD81 antigen CD81 Antibody

See all CD81 products

SKU/Catalog Number

A01281-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CD81 antigen CD81 Antibody catalog # A01281-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD81 antigen CD81 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01281-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human CD81.

Reactive Species

A01281-1 is reactive to CD81 in Human, Mouse, Rat

Applications

A01281-1 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For CD81 (Source: Uniprot.Org, NCBI)

Uniprot ID

P60033

Gene ID

975

Gene Name

CD81

Full Name

CD81 antigen

Weight

25.809kDa

Superfamily

tetraspanin (TM4SF) family

Alternative Names

CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7 CD81 CVID6, S5.7, TAPA1, TSPAN28 CD81 molecule CD81 antigen|26 kDa cell surface protein TAPA-1|CD81 antigen (target of antiproliferative antibody 1)|tetraspanin-28|tspan-28

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD81, check out the CD81 Infographic

CD81 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD81: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01281-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cao G,Meng X, Han X,Li J.Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a.Biosci Rep.2020 May 29;40(5): BSR20193436.doi:10.1042/BSR20193436.PMID:32400849;PMC
Species: Mouse
A01281-1 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELLS, DILUTION:NA

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15 Customer Q&As for Anti-CD81 antigen CD81 Antibody

Question

I am interested in using your anti-CD81 antibody for cellular response to low-density lipoprotein particle stimulus studies. Has this antibody been tested with western blotting on 4t1 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

Thanks for your inquiry. This A01281-1 anti-CD81 antibody is tested on 4t1 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-06

Question

Is this A01281-1 anti-CD81 antibody reactive to the isotypes of CD81?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

The immunogen of A01281-1 anti-CD81 antibody is Synthesized peptide derived from the Internal region of human CD81. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-26

Question

Do you have a BSA free version of anti-CD81 antibody A01281-1 available?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD81 antibody A01281-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-17

Question

I was wanting to use your anti-CD81 antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

You can see on the product datasheet, A01281-1 anti-CD81 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-21

Question

My colleagues were satisfied with the WB result of your anti-CD81 antibody. However we have been able to see positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is CD81 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

From what I have seen in literature, brain does express CD81. Generally CD81 expresses in cell membrane. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-11-14

Question

you antibody to test anti-CD81 antibody A01281-1 on rat brain for research purposes, then I may be interested in using anti-CD81 antibody A01281-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Wu

Verified customer

Asked: 2019-09-09

Answer

The products we sell, including anti-CD81 antibody A01281-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-09

Question

Will A01281-1 anti-CD81 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

You can see on the product datasheet, A01281-1 anti-CD81 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-21

Question

Can you help my question with product A01281-1, anti-CD81 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01281-1 anti-CD81 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-20

Question

See below the WB image, lot number and protocol we used for brain using anti-CD81 antibody A01281-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-13

Question

I see that the anti-CD81 antibody A01281-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-07

Question

Would anti-CD81 antibody A01281-1 work for WB with brain?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

According to the expression profile of brain, CD81 is highly expressed in brain. So, it is likely that anti-CD81 antibody A01281-1 will work for WB with brain.

Boster Scientific Support

Answered: 2019-06-06

Question

Is a blocking peptide available for product anti-CD81 antibody (A01281-1)?

Verified Customer

Verified customer

Asked: 2019-01-24

Answer

We do provide the blocking peptide for product anti-CD81 antibody (A01281-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-24

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CD81 antibody A01281-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-06-21

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-21

Question

We have observed staining in rat myometrium. What should we do? Is anti-CD81 antibody supposed to stain myometrium positively?

J. Moore

Verified customer

Asked: 2016-11-23

Answer

Based on literature myometrium does express CD81. Based on Uniprot.org, CD81 is expressed in myometrium, brain, among other tissues. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-11-23

Question

We are currently using anti-CD81 antibody A01281-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

G. Huang

Verified customer

Asked: 2016-09-28

Answer

The anti-CD81 antibody (A01281-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-09-28

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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A01281-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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