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Rat IL-4 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-4. 96wells/kit, with removable strips. Cited in 19 publication(s).
Rat IL-4 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0406 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Rat |
| Sample Type: | cell culture supernates and serum. |
| Sample Volume: | 100μl per well |
| Sensitivity: | <1pg/ml |
| Assay Range: | 15.6pg/ml-1000pg/ml |
Overview
| Product Name | Rat IL-4 ELISA Kit PicoKine™
See all Il4 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0406 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Rat IL4 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL4 tissue specificity: |
| Cite This Product | Rat IL-4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0406) |
Product Specs
| Reactivity/Species | Rat |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates and serum. |
| Sensitivity | <1pg/ml |
| Assay Range | 15.6pg/ml-1000pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: C25-S147 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P20096 |
|---|---|
| Gene Name | Il4 |
| Protein Name | Interleukin-4 |
| Subcellular Localization | Secreted. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Rat IL-4 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0406-CAP | 96-well plate precoated with anti-Rat Il4 antibody | 1 |
| EK0406-ST | lyophilized recombinant Rat Il4 standard | 10ng/tube |
| EK0406-DA | biotinylated anti-Rat Il4 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0406 Rat IL-4 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.068 | 0.107 | 0.143 | 0.229 | 0.470 | 0.793 | 1.561 | 2.163 |
We measured random samples of Rat IL-4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 35 | 164 | 456 | 32 | 175 | 481 |
| Standard deviation | 2.45 | 10.66 | 21.43 | 2.4 | 11.37 | 28.37 |
| CV(%) | 7% | 6.5% | 4.7% | 7.5% | 6.5% | 5.9% |
We measured the performance consistency of Rat IL-4 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 35 | 30 | 36 | 34 | 2.34 | 6.8% |
| Sample 2 | 164 | 156 | 144 | 155 | 154 | 7.11 | 4.6% |
| Sample 3 | 456 | 463 | 512 | 430 | 465 | 29.65 | 6.3% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 19
| Li L, Yu Ch, Ying Hz, Yu Jm. J Ethnopharmacol. 2013 May 2;147(1):238-44. Doi: 10.1016/J.Jep.2013.03.012. Epub 2013 Mar 15. Antiviral Effects Of Modified Dingchuan Decoction Against Respiratory Syncytial Virus Infection In Vitro And In An Immunosup... | PubMed ID 23506991 |
| Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ... | PubMed ID 23423691 |
| Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu... | PubMed ID 22464914 |
| Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (???????????? | PubMed ID 25428340 |
| Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro. | PubMed ID 20609400 |
| Li S, Hu X, Wang Z, Wu M, Zhang J. Inflamm Res. 2015 May;64(5):363-71. Doi: 10.1007/S00011-015-0816-Y. Epub 2015 Mar 27. Different Profiles Of Notch Signaling In Cigarette Smoke-Induced Pulmonary Emphysema And Bleomycin-Induced Pulmonary Fibrosis. | PubMed ID 25813392 |
| Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The... | PubMed ID 25744647 |
| Huang J, Wang P, Li M, Ge J, Chen J, Chen X. Int J Mol Med. 2015 Aug;36(2):493-500. Doi: 10.3892/Ijmm.2015.2249. Epub 2015 Jun 16. Trichostatin A Reduces Cisplatin-Induced Ototoxicity Through The Stat6 Signaling Pathway. | PubMed ID 26080623 |
| Wang Xj, Deng Hz, Jiang B, Yao H. Int J Colorectal Dis. 2012 May;27(5):575-81. Doi: 10.1007/S00384-011-1352-Z. Epub 2011 Nov 15. The Natural Plant Product Sophocarpine Ameliorates Dextran Sodium Sulfate-Induced Colitis In Mice By Regulating Cytoki... | PubMed ID 22083209 |
| Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert... | PubMed ID 24368037 |
| Qiu Jl, Zhu Wl, Lu Yj, Bai Zf, Liu Zg, Zhao P, Sun C, Zhang Yb, Li H, Liu W. Neurochem Int. 2015 Jun-Jul;85-86:46-52. Doi: 10.1016/J.Neuint.2015.04.007. Epub 2015 May 4. The Selective Mglur5 Agonist Chpg Attenuates So2-Induced Oxidative Stress And... | PubMed ID 25953665 |
| Jin Q, Cheng J, Liu Y, Wu J, Wang X, Wei S, Zhou X, Qin Z, Jia J, Zhen X. Brain Behav Immun. 2014 Aug;40:131-42. Doi: 10.1016/J.Bbi.2014.03.003. Epub 2014 Mar 12. Improvement Of Functional Recovery By Chronic Metformin Treatment Is Associated With... | PubMed ID 24632338 |
| Ou J, Shi W, Xu Y, Tao Z. Ann Allergy Asthma Immunol. 2014 Dec;113(6):658-665.E1. Doi: 10.1016/J.Anai.2014.08.015. Epub 2014 Sep 17. Intranasal Immunization With Dna Vaccine Coexpressing Der P 1 And Ubiquitin In An Allergic Rhinitis Mouse Model. | PubMed ID 25240330 |
| Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model. | PubMed ID 24738018 |
| Li J, Li Ff, Zuo W, Zhou Y, Hao Hy, Dang J, Jiang M, He Mz, Deng Dr. J Huazhong Univ Sci Technolog Med Sci. 2014 Aug;34(4):586-90. Doi: 10.1007/S11596-014-1320-2. Epub 2014 Aug 19. Up-Regulated Expression Of Tim-3/Gal-9 At Maternal-Fetal Interface... | PubMed ID 25135732 |
| Yuan Z, Zhang J, Yang C. Evid Based Complement Alternat Med. 2014;2014:620359. Doi: 10.1155/2014/620359. Epub 2014 Aug 17. Ligusticum Wallichii Extract Inhibited The Expression Of Il-1?? After Ami In Rats. | PubMed ID 25202335 |
| Tan Y, Liu H, Yang H, Wang L, Qin X. J Physiol Sci. 2013 Jan;63(1):63-9. Doi: 10.1007/S12576-012-0231-X. Epub 2012 Sep 26. An Inactivated Pseudomonas Aeruginosa Medicament Inhibits Airway Allergic Inflammation And Improves Epithelial Functions. | PubMed ID 23011634 |
| Song JW, Li K, Liang ZW, Dai C, Shen XF, Gong YZ, Wang S, Hu XY, Wang Z. Sci Rep. 2017 Apr 4;7(1):620. doi: 10.1038/s41598-017-00553-6. Low-level laser facilitates alternatively activated macrophage/microglia polarization and promotes functional r... | PubMed ID 28377600 |
| Treatment of surgical brain injury by immune tolerance induced by intrathymic and hepatic portal vein injection of brain antigens | PubMed ID 27554621 |
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Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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