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Product Info Summary
| SKU: | M01724 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD99 Mouse Monoclonal Antibody [Clone ID: OTI1D5]
SKU/Catalog Number
M01724
Size
100 µl
Description
Boster Bio CD99 mouse monoclonal antibody, clone OTI1D5 (formerly 1D5). Catalog# M01724. Tested in FC, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD99 Mouse Monoclonal Antibody [Clone ID: OTI1D5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01724)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1D5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CD99 (NP_002405) produced in HEK293T cell.
Reactive Species
M01724 is reactive to CD99 in Human
Calculated molecular weight
18.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01724 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Validation Images & Assay Conditions
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Flow cytometric Analysis of living Jurkat cells
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (M01724) Dilution: 1:150
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Western blot analysis of A549 cell lysate (35ug) by using anti-CD99 monoclonal antibody. Dilution: 1:500
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD99 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD99.
Specific Publications For Anti-CD99 Mouse Monoclonal Antibody [Clone ID: OTI1D5] (M01724)
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