Mouse CCL28 ELISA Kit PicoKine™

Mouse CCL28 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl28 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0691
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CCL28 ELISA Kit PicoKine™

See all Ccl28 products

SKU/Catalog Number

EK0691

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CCL28 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl28 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CCL28 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0691)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I23-R130

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0691 is reactive to Ccl28 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0691 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0691-CAP Anti-Mouse Ccl28 Pre-coated 96-well strip microplate 1
EK0691-ST Mouse Ccl28 Standard 2 vials, 10 ng/tube
EK0691-DA Mouse Ccl28 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)02000200020002000200020002000
O.D.0.0100.0920.1520.2900.5120.8971.5752.440

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CCL28 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7920983580199814
Standard deviation3.3911.0761.794.8812.9366.74
CV (%)4.3%5.3%7.4%6.1%6.5%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Ccl28 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 179849278835.536.6%
Sample 22092011872032008.064%
Sample 383575883190783252.696.3%
*number of samples for each test n=16.

Gene/Protein Information For Ccl28 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9JIL2

Gene ID

56838

Gene Name

Ccl28

Full Name

C-C motif chemokine 28

Weight

14.57kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC Ccl28|CCK1, MEC, Scya, Scya28|chemokine (C-C motif) ligand 28|C-C motif chemokine 28|small-inducible cytokine A28

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccl28, check out the Ccl28 Infographic

Ccl28 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccl28: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse CCL28 ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

S. Dhar

Verified customer

Asked: 2019-08-02

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-08-02

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-07-19

Question

Q: how to thaw whole blood sample for CCL28 ELISA after freezing?

C. Jones

Verified customer

Asked: 2019-03-26

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL28 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-03-26

Question

Q: What is the optimal O.D. value for CCL28 ELISA kit? I performed your CCL28 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL28 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-03-20

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL28 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CCL28 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-03-20

Question

Q: if the enzyme conjugated CCL28 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL28 antigen?

H. Banerjee

Verified customer

Asked: 2019-01-01

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-01-01

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CCL28 using the EK0691 Mouse CCL28 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-10-11

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-10-11

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

E. Davis

Verified customer

Asked: 2016-01-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-01-14

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