Human Annexin A1 ELISA Kit PicoKine™

Human Annexin A1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANXA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 100pg/ml.

Product Info Summary

SKU: EK1745
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Product Name

Human Annexin A1 ELISA Kit PicoKine™

See all Annexin A1 products

SKU/Catalog Number

EK1745

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Annexin A1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANXA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 100pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Annexin A1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1745)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-N346

Sensitivity

<100 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1745 is reactive to ANXA1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Application Guarantee

EK1745 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1745-CAP Anti-Human ANXA1 Pre-coated 96-well strip microplate 1
EK1745-ST Human ANXA1 Standard 2 vials, 10 ng/tube
EK1745-DA Human ANXA1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0210.0660.1960.2450.4520.8611.4371.943

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Annexin A1, Dilution ratio of 1:10 1:500, concentration in serum is around 20ng/ml around 1ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Annexin A1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2991474520930314995311
Standard deviation12.5664.86276.117.992.9414.3
CV (%)4.2%4.4%5.3%5.9%6.2%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Annexin A1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12992822932992936.942.3%
Sample 21474142513891444143330.832.1%
Sample 352094826501649925010135.842.7%
*number of samples for each test n=16.

Gene/Protein Information For ANXA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04083

Gene ID

301

Gene Name

ANXA1

Full Name

Annexin A1

Weight

38.714kDa

Superfamily

annexin family

Alternative Names

Annexin A1; annexin I (lipocortin I); Annexin I; Annexin-1; ANX1; ANXA1; Calpactin II; calpactin-2; chromobindin-9; Lipocortin I; LPC1; LPC1annexin-1; p35; Phospholipase A2 inhibitory protein ANXA1 ANX1, LPC1 annexin A1 annexin A1|annexin I (lipocortin I)|annexin-1|calpactin II|calpactin-2|chromobindin-9|epididymis secretory sperm binding protein|phospholipase A2 inhibitory protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANXA1, check out the ANXA1 Infographic

ANXA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANXA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Human Annexin A1 ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-08-17

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-08-17

Question

Q: Can Annexin A1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

A. Green

Verified customer

Asked: 2019-01-13

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-01-13

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-04-30

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-04-30

Question

Q: can I use citrate plasma as samples in Human Annexin A1 Picokine® ELISA Kit (Catalog # EK1745)?

H. Wu

Verified customer

Asked: 2018-03-25

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Annexin A1 causing degradation of its protein structure. Annexin A1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Annexin A1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-03-25

Question

Q: which procedure should I follow in order to thaw whole blood sample for Annexin A1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-05-01

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Annexin A1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-05-01

Question

Q: if the enzyme conjugated Annexin A1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Annexin A1 antigen?

T. King

Verified customer

Asked: 2015-12-24

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2015-12-24

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