Anti-Cdc2 P34 Antibody

Product Name

Anti-Cdc2 P34 Antibody

View all CDC2/CDK1 Antibodies

SKU/Catalog Number

A30951

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Cdc2 P34 Antibody (Catalog # A30951). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Cdc2 P34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30951)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

CDC2 peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30951 is reactive to CDK1 in Human

Reconstitution

Calculated molecular weight

114443 MW

Background of CDC2/CDK1

p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphorylation appears to be performed by p60src. The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A30951 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:60,000
IHC: 1:200 - 1:1,000
IP: 1:100
WB: 1:250 - 1:5,000

Validation Images & Assay Conditions

Gene/Protein Information For CDK1 (Source: Uniprot.org, NCBI)

Alternative Names

Cdc2, p34, Cell Division Cycle 2 Protein, Cyclin Dependent Kinase 1, p34 Cdk1, p34 protein kinase, Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK1, CDC2, CDC28A, CDKN1, P34CDC2 CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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