Anti-Cdc2 P34 Antibody

Product Name

Anti-Cdc2 P34 Antibody

View all CDK1 Antibodies

SKU/Catalog Number

A30951

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Cdc2 P34 Antibody (Catalog # A30951). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Cdc2 P34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30951)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

CDC2 peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Reactive Species

A30951 is reactive to CDK1 in Human

Calculated molecular weight

34.1 kDa

Background of CDK1

p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphorylation appears to be performed by p60src. The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A30951 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:60,000
IHC: 1:200 - 1:1,000
IP: 1:100
WB: 1:250 - 1:5,000
Anti-p34 cdc2 antibody has been tested in ELISA and western blot and is suitable for immunoblotting, immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting.  HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting.  LEP fibroblast cell lysate is suggested as a negative control. This product is suitable for the detection by immunoblot of human, rat and mouse cdc2. For immunohistochemistry use paraffin embedded tissue.

Validation Images & Assay Conditions

See full target information CDK1

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