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Product Info Summary
| SKU: | A13764-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cdc50B TMEM30B Antibody
SKU/Catalog Number
A13764-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cdc50B TMEM30B Antibody catalog # A13764-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdc50B TMEM30B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13764-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TMEM30B. AA range:91-140
Reactive Species
A13764-1 is reactive to TMEM30B in Human, Mouse
Calculated molecular weight
38.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13764-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562 cells using Cdc50B Polyclonal antibody.
Specific Publications For Anti-Cdc50B TMEM30B Antibody (A13764-1)
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Customer Q&As
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5 Customer Q&As for Anti-Cdc50B TMEM30B Antibody
Question
Will A13764-1 anti-Cdc50B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
It shows on the product datasheet, A13764-1 anti-Cdc50B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-04
Question
Is this A13764-1 anti-Cdc50B antibody reactive to the isotypes of TMEM30B?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
The immunogen of A13764-1 anti-Cdc50B antibody is Synthesized peptide derived from the Internal region of human Cdc50B. at AA rangle: 60-140. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-18
Question
My question regarding product A13764-1, anti-Cdc50B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13764-1 anti-Cdc50B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-Cdc50B antibody A13764-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The anti-Cdc50B antibody (A13764-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
Question
Is a blocking peptide available for product anti-Cdc50B antibody (A13764-1)?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
We do provide the blocking peptide for product anti-Cdc50B antibody (A13764-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-06-04
