Anti-Mesothelin/MSLN Antibody Picoband™

Mesothelin antibody

Boster Bio Anti-Mesothelin/MSLN Antibody Picoband™ catalog # PB9287. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9287
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB

Product Name

Anti-Mesothelin/MSLN Antibody Picoband™

View all Mesothelin Antibodies

SKU/Catalog Number

PB9287

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mesothelin/MSLN Antibody Picoband™ catalog # PB9287. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mesothelin/MSLN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9287)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9287 is reactive to MSLN in Human

Applications

PB9287 is guaranteed for ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

69 kDa

Calculated molecular weight

68.986kDa

Background of Mesothelin

Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For MSLN (Source: Uniprot.org, NCBI)

Gene Name

MSLN

Full Name

Mesothelin

Weight

68.986kDa

Superfamily

mesothelin family

Alternative Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein MSLN MPF, SMRP mesothelin mesothelin|CAK1 antigen|megakaryocyte potentiating factor|pre-pro-megakaryocyte-potentiating factor|soluble MPF mesothelin related protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MSLN, check out the MSLN Infographic

MSLN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MSLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-Mesothelin/MSLN Antibody Picoband™

Question

See attached the WB image, lot number and protocol we used for pancreatic tumor using anti-Mesothelin/MSLN antibody PB9287. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-05-08

Question

I am interested in using your anti-Mesothelin/MSLN antibody for post-translational protein modification studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

I appreciate your inquiry. This PB9287 anti-Mesothelin/MSLN antibody is tested on mesothelin cells, human hela, lung cancer tissue, siha cells, hela whole cell lysates. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-30

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreatic tumor using anti-Mesothelin/MSLN antibody PB9287. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-06

Question

My colleagues were content with the WB result of your anti-Mesothelin/MSLN antibody. However we have been able to see positive staining in carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is MSLN supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

Based on literature, carcinoma does express MSLN. Generally MSLN expresses in cell membrane. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035

Boster Scientific Support

Answered: 2020-01-13

Question

We have observed staining in human right uterine tube. What should we do? Is anti-Mesothelin/MSLN antibody supposed to stain right uterine tube positively?

H. Moore

Verified customer

Asked: 2019-12-03

Answer

Based on literature right uterine tube does express MSLN. Based on Uniprot.org, MSLN is expressed in right uterine tube, pancreatic cancer, carcinoma, lung spleen, pancreas placenta, pancreatic tumor, tear, among other tissues. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035

Boster Scientific Support

Answered: 2019-12-03

Question

I see that the anti-Mesothelin/MSLN antibody PB9287 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-25

Question

Does anti-Mesothelin/MSLN antibody PB9287 work for ELISA with pancreatic tumor?

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

According to the expression profile of pancreatic tumor, MSLN is highly expressed in pancreatic tumor. So, it is likely that anti-Mesothelin/MSLN antibody PB9287 will work for ELISA with pancreatic tumor.

Boster Scientific Support

Answered: 2019-10-18

Question

Do you have a BSA free version of anti-Mesothelin/MSLN antibody PB9287 available?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Mesothelin/MSLN antibody PB9287 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-30

Question

We are currently using anti-Mesothelin/MSLN antibody PB9287 for human tissue, and we are satisfied with the IHC-F results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

R. Lewis

Verified customer

Asked: 2019-06-17

Answer

The anti-Mesothelin/MSLN antibody (PB9287) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-17

Question

Is a blocking peptide available for product anti-Mesothelin/MSLN antibody (PB9287)?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

We do provide the blocking peptide for product anti-Mesothelin/MSLN antibody (PB9287). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-28

Question

Is this PB9287 anti-Mesothelin/MSLN antibody reactive to the isotypes of MSLN?

Verified Customer

Verified customer

Asked: 2019-01-25

Answer

The immunogen of PB9287 anti-Mesothelin/MSLN antibody is E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-25

Question

My question regarding product PB9287, anti-Mesothelin/MSLN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-12

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9287 anti-Mesothelin/MSLN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-12

Question

Does PB9287 anti-Mesothelin/MSLN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

As indicated on the product datasheet, PB9287 anti-Mesothelin/MSLN antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-01-09

Question

We bought anti-Mesothelin/MSLN antibody for ELISA on lung spleen in the past. I am using human, and We are going to use the antibody for WB next. We are interested in examining lung spleen as well as tear in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-01-04

Answer

I have checked the website and datasheets of our anti-Mesothelin/MSLN antibody and I see that PB9287 has been validated on human in both ELISA and WB. Thus PB9287 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-01-04

Question

I was wanting to use your anti-Mesothelin/MSLN antibody for ELISA for human pancreatic tumor on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreatic tumor identification?

M. Mangal

Verified customer

Asked: 2017-09-18

Answer

As indicated on the product datasheet, PB9287 anti-Mesothelin/MSLN antibody has been validated for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreatic tumor in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-18

Question

you antibody to test anti-Mesothelin/MSLN antibody PB9287 on human pancreatic tumor for research purposes, then I may be interested in using anti-Mesothelin/MSLN antibody PB9287 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-08-08

Answer

The products we sell, including anti-Mesothelin/MSLN antibody PB9287, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-08

Order DetailsPrice
PB9287

100μg

$370
PB9287-10ug

10μg sample (liquid)

$99
PB9287-Biotin

100 μg Biotin conjugated

$570
PB9287-Cy3

100 μg Cy3 conjugated

$570
PB9287-Dylight488

100 μg Dylight488 conjugated

$570
PB9287-Dylight550

100 μg Dylight550 conjugated

$570
PB9287-Dylight594

100 μg Dylight594 conjugated

$570
PB9287-FITC

100 μg FITC conjugated

$570
PB9287-HRP

100 μg HRP conjugated

$570
PB9287-APC

100 μg APC conjugated

$670
PB9287-PE

100 μg PE conjugated

$670
PB9287-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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