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Product Info Summary
| SKU: | EK1338 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. |
Product info
Product Name
Human Mesothelin ELISA Kit PicoKine®
SKU/Catalog Number
EK1338
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
MSLN (mesothelin) is a GPI-anchored cell-surface glycoprotein that may contribute to cell adhesion (e.g., reported binding to MUC16/CA125 in cancer biology); it is widely used as a tumor-associated antigen/biomarker in translational oncology contexts (interpretation depends on sample/source). Assay context: PicoKine ELISA for human biofluids with sensitivity <10 pg/mL and detection range 156–10,000 pg/mL, suited to quantitative workflows in ELISA testing and broader panel building via multiplex assay services (context-dependent). Often considered alongside immuno-oncology and tumor burden markers such as Human PD-L1 ELISA and tumor biology markers like p53 or CEA quick ELISA (panel selection is study-specific).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Mesothelin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1338)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E296-G580
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1338 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1338 is reactive to MSLN in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EK1338 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MSLN
Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1338-CAP | Anti-Human MSLN Pre-coated 96-well strip microplate | 1 |
| EK1338-ST | Human MSLN Standard | 2 vials, 10 ng/tube |
| EK1338-DA | Human MSLN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.051 | 0.151 | 0.199 | 0.331 | 0.548 | 0.932 | 1.622 | 2.269 |
Data Example Images
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Human Mesothelin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MSLN, Dilution ratio of 1:10, concentration in serum and plasma is around 7.7-48.24 ng/ml..
Some articles we found to cite concentrations of MSLN in samples: 25477701 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Mesothelin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 307 | 1077 | 6075 | 320 | 1138 | 6301 |
| Standard deviation | 12.58 | 52.77 | 443.47 | 17.28 | 70.55 | 516.68 |
| CV (%) | 4.1% | 4.9% | 7.3% | 5.4% | 6.2% | 8.2% |
Specific Publications For Human Mesothelin ELISA Kit PicoKine® (EK1338)
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Customer Reviews
0 Reviews For Human Mesothelin ELISA Kit PicoKine®
Customer Q&As
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8 Customer Q&As for Human Mesothelin ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Baker
Verified customer
Asked: 2020-07-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-03
Question
Q: Can Mesothelin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-03-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-03-07
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Mesothelin using the EK1338 Human Mesothelin Picokine® ELISA Kit?
G. White
Verified customer
Asked: 2019-12-31
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-12-31
Question
Q: how do I analyze ELISA data? I have obtained Mesothelin level in serum.
Verified Customer
Verified customer
Asked: 2019-10-20
Answer
A: please read this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2019-10-20
Question
Q: if the enzyme conjugated Mesothelin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Mesothelin antigen?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-09-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
L. Thompson
Verified customer
Asked: 2018-11-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-09
Question
Q: What is the optimal O.D. value for Mesothelin ELISA kit? I performed your Mesothelin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Mesothelin even though the O.D. values are not very high?
J. Yang
Verified customer
Asked: 2018-11-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Mesothelin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Mesothelin that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-11-05
Question
Q: is it okay to use citrate plasma as samples in Human Mesothelin Picokine® ELISA Kit (Catalog # EK1338)?
C. Walker
Verified customer
Asked: 2018-08-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Mesothelin causing degradation of its protein structure. Mesothelin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Mesothelin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-04
