Human Mesothelin ELISA Kit PicoKine™

Human Mesothelin ELISA Kit PicoKine™ (96 Tests). Quantitate Human MSLN in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1338
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Product Name

Human Mesothelin ELISA Kit PicoKine™

See all Mesothelin products

SKU/Catalog Number

EK1338

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Mesothelin ELISA Kit PicoKine™ (96 Tests). Quantitate Human MSLN in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Mesothelin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1338)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E296-G580

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1338 is reactive to MSLN in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Application Guarantee

EK1338 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1338-CAP Anti-Human MSLN Pre-coated 96-well strip microplate 1
EK1338-ST Human MSLN Standard 2 vials, 10 ng/tube
EK1338-DA Human MSLN Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010,000
O.D.0.0410.1250.1900.2820.4950.8151.2371.772

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Mesothelin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3071077607532011386301
Standard deviation12.5852.77443.4717.2870.55516.68
CV (%)4.1%4.9%7.3%5.4%6.2%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Mesothelin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 130728230734431022.127.1%
Sample 21077108212231218115070.546.1%
Sample 360755852550057765800205.483.5%
*number of samples for each test n=16.

Gene/Protein Information For MSLN (Source: Uniprot.Org, NCBI)

Uniprot ID

Q13421

Gene ID

10232

Gene Name

MSLN

Full Name

Mesothelin

Weight

68.986kDa

Superfamily

mesothelin family

Alternative Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein MSLN MPF, SMRP mesothelin mesothelin|CAK1 antigen|megakaryocyte potentiating factor|pre-pro-megakaryocyte-potentiating factor|soluble MPF mesothelin related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MSLN, check out the MSLN Infographic

MSLN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MSLN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1338

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8 Customer Q&As for Human Mesothelin ELISA Kit PicoKine™

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

M. Baker

Verified customer

Asked: 2020-07-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-07-03

Question

Q: Can Mesothelin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-03-07

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-03-07

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Mesothelin using the EK1338 Human Mesothelin Picokine® ELISA Kit?

G. White

Verified customer

Asked: 2019-12-31

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-12-31

Question

Q: how do I analyze ELISA data? I have obtained Mesothelin level in serum.

Verified Customer

Verified customer

Asked: 2019-10-20

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-10-20

Question

Q: if the enzyme conjugated Mesothelin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Mesothelin antigen?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-09-13

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

L. Thompson

Verified customer

Asked: 2018-11-09

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-11-09

Question

Q: What is the optimal O.D. value for Mesothelin ELISA kit? I performed your Mesothelin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Mesothelin even though the O.D. values are not very high?

J. Yang

Verified customer

Asked: 2018-11-05

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Mesothelin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Mesothelin that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-11-05

Question

Q: is it okay to use citrate plasma as samples in Human Mesothelin Picokine® ELISA Kit (Catalog # EK1338)?

C. Walker

Verified customer

Asked: 2018-08-04

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Mesothelin causing degradation of its protein structure. Mesothelin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Mesothelin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-08-04

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