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| SKU | MA1019 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human |
| Clonality | Monoclonal |
| Clone Number | DCS-180 |
| Host | Mouse |
| Ig Isotype | Mouse IgG1 |
| Applications | WB |
Overview
| Product Name | Anti-CDC6 Antibody (Monoclonal, DCS-180) |
|---|---|
| SKU/Catalog Number | MA1019 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S. cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivity with other proteins. |
| Cite This Product | Anti-CDC6 Antibody (Monoclonal, DCS-180) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1019) |
| Host | Mouse |
| Immunogen | Recombinant human Cdc6. |
| Reactivity | Human |
Assay Details
Assay Dilutions Overview
Western blot, 1-2μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | O89033 |
|---|---|
| Gene Name | Cdc6 |
| Protein Name | Cell division control protein 6 homolog |
| Alternative Names | Cell division control protein 6 homolog;CDC6-related protein;p62(cdc6);Cdc6; |
| Subcellular Localization | Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells. . |
| Molecular Weight | 62614 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. |
|---|---|
| Background | In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al.(1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells. |
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Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p62 antibody, cdc6 antibody