| SKU: | MA1019 |
|---|---|
| Pack Size: | 100μg/vial |
| Validated Species: | Human |
| Clonality: | Monoclonal |
| Clone Number: | DCS-180 |
| Host: | Mouse |
| Application: | WB |
- Technical
Specs - Kit
Components - Gene
Information - Application
Data & Images - Reviews
Publications - Customer
Q&As
Overview
| Product Name | Anti-CDC6 Antibody (Monoclonal, DCS-180) |
|---|---|
| SKU/Catalog Number | MA1019 |
| Description | Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S. cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivity with other proteins. |
| Cite This Product | Anti-CDC6 Antibody (Monoclonal, DCS-180) DCS-180 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1019) |
| Replacement Item | This antibody may replace the following items: sc-70826|sc-70827|sc-28553|sc-8341|sc-56273|sc-13136|sc-32507|sc-9964|sc-6318|sc-6317 from Santa Cruz Biotechnology. |
| Host | Mouse |
| Validated Species | Human |
| Application | WB *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Recommended Detection Systems | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot. *Blocking peptide can be purchased at $50. Contact us for more information **Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018! |
| Immunogen | Recombinant human Cdc6. |
| Cross Reactivity | No cross reactivity with other proteins |
| Pack Size | 100μg/vial |
Properties
| Clonality | Monoclonal |
|---|---|
| Clone Number | DCS-180 |
| Form | Lyophilized |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Concentration | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Purification | Ascites |
| Isotype | Mouse IgG1 |
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | Cdc6 |
|---|---|
| Protein Name | Cell division control protein 6 homolog |
| Molecular Weight | 62614 MW |
| Protein Function | Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. |
| Sequence Similarities | Belongs to the CDC6/cdc18 family. |
| Subcellular Localization | Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells. . |
| Uniprot ID | O89033 |
| Alternative Names | Cell division control protein 6 homolog;CDC6-related protein;p62(cdc6);Cdc6; |
| Research Areas | |epigenetics and nuclear signaling|dna / rna|dna synthesis| cell biology|cell cycle|cell division|other cell division antibodies| |
Background for Cell division control protein 6 homolog
In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al.(1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
Related Products
Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate (BA1054)
Price: FREE if purchase with Primary antibody
Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001)
Price: $100
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p62 antibody, cdc6 antibody
