Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM5(MCM5) detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
Anti-MCM5 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||Flow Cytometry, IF, IHC-P, ICC, WB|
GMP synthase (GMPS) is involved in purine biosynthesis. GMPS, which is a homodimer, catalyzes the last step in the GMP synthesis pathway, specifically the ATP-dependent amination of XMP to GMP. GMPS is comprised of one GMP-binding domain and one glutamine amidotransferase type-1 domain through which it communicates its catalytic activity. GMPS is engaged in the de novo synthesis of guanine nucleotides which are not only vital for DNA and RNA synthesis, but also supply GTP, which is involved in sevral cellular processes important for cell division. GMPS gene chromosomal translocations are linked with acute myeloid leukemias, suggesting a possible role for GMPS in carcinogenesis.
|Product Name||Anti-MCM5 Antibody
See all MCM5 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MCM5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MCM5 information: Molecular Weight: 82286 MW; Subcellular Localization: Nucleus.|
|Cite This Product||Anti-MCM5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1653)|
|Similar Products From
|Anti-MCM5 Antibody may replace the following items: sc 165995|sc 165994|sc 8281|sc 22780|sc 165993|sc 136366|sc 6686|sc 6687.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||Flow Cytometry, IF, IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5(720-734aa EIQHRMQRKVLYRLK), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MCM5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4174|
|Species Of This Entry||Human|
|Protein Name||DNA replication licensing factor MCM5|
|Alternative Names||MCM5|CDC46 homolog; CDC46DNA replication licensing factor MCM5; EC 184.108.40.206; MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae); MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGC5315; minichromosome maintenance complex component 5; minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46); minichromosome maintenance deficient 5 (cell division cycle 46); P1-CDC46|
|Post Tranlational Modifications||Phosphorylation; Dephosphorylation; Biotinylation; Acetylation; Methylation|
|Subcellular Localization||Nucleus. Cytoplasm, cytosol.|
|Gene Location||22q12.3, on Chromasome 22, gene sequence: NC_000022.11 (35400133..35454942)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MCM5 (Source: Uniprot.org, NCBI)
|Protein Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP. .|
|Interacting Proteins||HIST1H4A; L3MBTL1; MCM2; MCM3; MCM7; MCMBP|
|Research Areas||Cancer; Cell Cycle and Replication; Core ESC-Like Genes; DNA Repair; Stem Cell Markers|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Urinary Bladder; Dysplasia; Cell Transformation, Neoplastic; Adenocarcinoma; Bladder Neoplasm; Malignant Squamous Cell Neoplasm; Uterine Cervical Neoplasm; Malignant Tumor Of Cervix; Malignant Neoplasm Of Prostate; Retinoblastoma; Intraepithelial Neoplasia; Genomic Instability; Cervical Intraepithelial Neoplasia; Esophageal Neoplasms; Prostatic Neoplasms; Neoplasm Metastasis|
|Related Pathways||Dna Replication; Cell Cycle; S Phase; Cell Proliferation; Localization; Mitosis; G1 Phase; M Phase; Cell Division; Cell Cycle Arrest; Regulation Of Dna Replication; Cell Death; Cell Growth; Dna Replication Initiation; Interphase; Pathogenesis; Dna Repair; Single-stranded Dna Binding; Neurogenesis; Anaphase|
|Background||MCM5(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 5),also known as CDC46, is a protein that in humans is encoded by the MCM5 gene. MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. And MCM5 is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The MCM5 gene is mapped on 22q12.3. The MCM5 gene contains 16 exons. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.|
|Scientific References||PMID: 8265339 by Hu B., et al. The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins.|
PMID: 16899510 by Tsuji T., et al. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells.
Our Boster Quality Guarantee for Anti-MCM5 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate Lane3: JURKAT Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- MCM5 antigen affinity purified polyclonal antibody (Catalog # PA1653) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM5 at approximately 82KD. The expected band size for MCM5 is at 82KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (PA1653)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.