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Product Info Summary
| SKU: | PA1653 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MCM5 Antibody Picoband®
SKU/Catalog Number
PA1653
BA3685 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCM5 Antibody catalog # PA1653. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCM5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1653)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1653 is reactive to MCM5 in Human, Mouse, Rat
Observed Molecular Weight
82 kDa
Calculated molecular weight
82.3 kDa
Background of MCM5
MCM5 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 5),also known as CDC46, is a protein that in humans is encoded by the MCM5 gene. MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. And MCM5 is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The MCM5 gene is mapped on 22q12.3. The MCM5 gene contains 16 exons. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1653 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: Rat Testis Tissue, Rat Brain Tissue Lane3: JURKAT Cell,, human Jurkat whole cell,
IHC: Human Intestinal Cancer Tissue
ICC/IF: U20S cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Anti-MCM5 antibody, PA1653, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate Lane3: JURKAT Cell Lysate
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Anti-MCM5 antibody, PA1653, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Western blot analysis of MCM5 using anti-MCM5 antibody (PA1653).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM5 antigen affinity purified polyclonal antibody (Catalog # PA1653) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM5 at approximately 82KD. The expected band size for MCM5 is at 82KD.
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IF analysis of MCM5 using anti-MCM5 antibody (PA1653).
MCM5 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCM5 Antibody (PA1653) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of HL-60 cells using anti-MCM5 antibody (PA1653).
Overlay histogram showing HL-60 cells stained with PA1653 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM5 Antibody (PA1653, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-MCM5 Antibody Picoband® (PA1653)
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3 Customer Q&As for Anti-MCM5 Antibody Picoband®
Question
We need to test anti-MCM5 antibody PA1653 on mouse erythroleukemia for research purposes, then I may be interested in using anti-MCM5 antibody PA1653 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The products we sell, including anti-MCM5 antibody PA1653, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-24
Question
Is this PA1653 anti-MCM5 antibody reactive to the isotypes of MCM5?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The immunogen of PA1653 anti-MCM5 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5(720-734aa EIQHRMQRKVLYRLK), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-27
Question
We are currently using anti-MCM5 antibody PA1653 for mouse tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
S. Anderson
Verified customer
Asked: 2019-09-20
Answer
The anti-MCM5 antibody (PA1653) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-20
