Anti-MCM5 Antibody

MCM5 antibody

Boster Bio Anti-MCM5 Antibody catalog # PA1653. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1653
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-MCM5 Antibody

View all MCM5 Antibodies

SKU/Catalog Number

PA1653

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MCM5 Antibody catalog # PA1653. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MCM5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1653)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1653 is reactive to MCM5 in Human, Mouse, Rat

Applications

PA1653 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

82 kDa

Calculated molecular weight

82.286kDa

Background of MCM5

MCM5 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 5),also known as CDC46, is a protein that in humans is encoded by the MCM5 gene. MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. And MCM5 is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The MCM5 gene is mapped on 22q12.3. The MCM5 gene contains 16 exons. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For MCM5 (Source: Uniprot.org, NCBI)

Gene Name

MCM5

Full Name

DNA replication licensing factor MCM5

Weight

82.286kDa

Superfamily

MCM family

Alternative Names

CDC46 homolog; CDC46DNA replication licensing factor MCM5; EC 3.6.4.12; MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae); MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGC5315; minichromosome maintenance complex component 5; minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46); minichromosome maintenance deficient 5 (cell division cycle 46); P1-CDC46 MCM5 CDC46, MGORS8, P1-CDC46 minichromosome maintenance complex component 5 DNA replication licensing factor MCM5|CDC46 homolog|MCM5 minichromosome maintenance deficient 5, cell division cycle 46|minichromosome maintenance deficient 5 (cell division cycle 46)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MCM5, check out the MCM5 Infographic

MCM5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MCM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-MCM5 Antibody

Question

We need to test anti-MCM5 antibody PA1653 on mouse erythroleukemia for research purposes, then I may be interested in using anti-MCM5 antibody PA1653 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

The products we sell, including anti-MCM5 antibody PA1653, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-24

Question

Is this PA1653 anti-MCM5 antibody reactive to the isotypes of MCM5?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

The immunogen of PA1653 anti-MCM5 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5(720-734aa EIQHRMQRKVLYRLK), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-27

Question

We are currently using anti-MCM5 antibody PA1653 for mouse tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

S. Anderson

Verified customer

Asked: 2019-09-20

Answer

The anti-MCM5 antibody (PA1653) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-20

Order DetailsPrice
PA1653

100μg

$370
PA1653-10ug

10μg sample (liquid)

$99
PA1653-Biotin

100 μg Biotin conjugated

$570
PA1653-Cy3

100 μg Cy3 conjugated

$570
PA1653-Dylight488

100 μg Dylight488 conjugated

$570
PA1653-Dylight550

100 μg Dylight550 conjugated

$570
PA1653-Dylight594

100 μg Dylight594 conjugated

$570
PA1653-FITC

100 μg FITC conjugated

$570
PA1653-HRP

100 μg HRP conjugated

$570
PA1653-APC

100 μg APC conjugated

$670
PA1653-PE

100 μg PE conjugated

$670
PA1653-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1653
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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