Overview
| Product Name |
Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1)
See all Cdk7 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
MA1022 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description |
Monoclonal clone# G2 antibody for CDK7 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-F. Reactive species: Human. CDK7 information: Molecular Weight: 37141 MW; Subcellular Localization: Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Colocalizes with PRKCI in the cytoplasm and nucleus. Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides (By similarity); Tissue Specificity: CDK7: Ubiquitous. |
| Cite This Product |
Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1022)
|
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Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1) may replace the following items: sc 723|sc 856|sc 365075|sc 7344|sc 529|sc 393308|sc 22119|sc 22120|sc 56284|sc 271794|sc 856 X. |
Product Specs
| Host |
Mouse |
| Reactivity/Species |
Human |
| Predicted Reactivity |
Monkey
*This antibody is predicted to react with the above species based on antigen
sequence similarities. Our Boster Guarantee covers the use of this product with the above species. |
| Applications |
IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
Recombinant human Cdk7 protein. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For Cdk7 (Source: Uniprot.org, NCBI)
| Uniprot Id | P51952 |
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| NCBI Gene Id | 171150 |
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| Species Of This Entry | Rat |
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| Gene Name | Cdk7 |
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| Protein Name | Cyclin-dependent kinase 7 |
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| Superfamily | protein kinase superfamily |
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| Alternative Names | Cdk7|CAK1Xenopus laevis, cdk-activating kinase); CDK-activating kinase; Cell division protein kinase 7; cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase); cyclin-dependent kinase 7; EC 2.7.11; EC 2.7.11.22; EC 2.7.11.23; EC 2.7.11.24; p39 Mo15; serine/threonine kinase stk1,39 kDa protein kinase; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; TFIIH basal transcription factor complex kinase subunit |
|---|
| Molecular Weight | 37141 |
|---|
| Post Tranlational Modifications | Phosphorylation; Dephosphorylation; Cleavage; Methylation; Acetylation; Sumoylation; Reduction; Phosphorothioation; Deacetylation; Geranylgeranylation; Ubiquitination |
|---|
| Gene Location | 2q12, on Chromasome 2, gene sequence: |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Cdk7 (Source: Uniprot.org, NCBI)
| Protein Function | Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin- dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition (By similarity). . |
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| Research Areas | Cell Cycle and Replication; DNA Repair; Nucleotide Excision Repair; Signal Transduction; Transcription Factors and Regulators |
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| Related Diseases | Malignant Neoplasms; Neoplasms; Carcinoma; Retinoblastoma; Malignant Paraganglionic Neoplasm; Xeroderma; Leukemia; Xeroderma Pigmentosum; Mammary Neoplasms; Hiv Infections; Immunologic Deficiency Syndromes; Malignant Neoplasm Of Breast; Liver Carcinoma; Liver Neoplasms; Lymphoid Leukemia; Chronic Lymphocytic Leukemia; Lung Neoplasms; Lymphoma; Cell Transformation, Neoplastic; Malignant Neoplasm Of Lung |
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| Related Pathways | Cell Cycle; Dna Repair; Cell Cycle Arrest; Cell Proliferation; Cell Division; Mitosis; Localization; Cell Growth; Hyperphosphorylation; Cell Death; S Phase; G1 Phase; Induction Of Apoptosis; Dna Replication; Translation; M Phase; Protein Phosphorylation; Cell Cycle Phase; Aging; Reverse Transcription |
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| Background | CDK-activating kinases(CAKs) are multisubunit proteins that phosphorylate and thus activate certain cyclin-dependent protein kinases in the regulation of cell cycle progression. Cyclin dependent kinase7(CDK7) gene is mapped to chromosome 2p15-cen. CDK7 functions in both cyclin binding and T-loop phosphorylation and that these 2 steps of CDK1 activation are mutually dependent. |
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| Scientific References | PMID: 22673903 by Lundby A., et al. Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. |
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Our Boster Quality Guarantee for Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1) covers its use in the following applications.
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5μg/ml, Human, -
Western blot, 0.25μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-Cdk7/CAK antibody (monoclonal), MA1022, IHC(P)
IHC(P): Human Breast Tissue
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Cdk7 primary antibodies,
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