|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1)|
|Description||Mouse IgG monoclonal antibody for Cdk7/CAK, cyclin-dependent kinase 7 (CDK7) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1) MO-1.1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1022)|
|Replacement Item||This antibody may replace the following items: sc-723|sc-856|sc-365075|sc-7344|sc-529|sc-393308|sc-22119|sc-22120|sc-56284|sc-271794|sc-856-X from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Recombinant human Cdk7 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase 7|
|Molecular Weight||37141 MW|
|Protein Function||Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin- dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition (By similarity). .|
|Tissue Specificity||CDK7: Ubiquitous.|
|Sequence Similarities||Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.|
|Subcellular Localization||Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Colocalizes with PRKCI in the cytoplasm and nucleus. Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides (By similarity). .|
|Alternative Names||Cyclin-dependent kinase 7;220.127.116.11;18.104.22.168;39 protein kinase;P39 Mo15;CDK-activating kinase 1;Cell division protein kinase 7;TFIIH basal transcription factor complex kinase subunit;Cdk7;Cak, Cak1, Mo15;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases|cdks| epigenetics and nuclear signaling||