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Product Info Summary
| SKU: | PB9536 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cdk7 Antibody Picoband®
SKU/Catalog Number
PB9536
PB0564 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cdk7 Antibody Picoband® catalog # PB9536. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdk7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9536)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cdk7 recombinant protein (Position: D31-F346). Human Cdk7 shares 94.9% and 96.1% amino acid (aa) sequence identity with mouse and rat Cdk7, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9536 is reactive to CDK7 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.0 kDa
Background of CDK7
Cyclin-dependent kinase 7, also known as cell division protein kinase 7, is an enzyme that in humans is encoded by the CDK7 gene. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. The gene was assigned to human chromosome 5q13.2. Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. It is required for DNA-bound peptides-mediated transcription and cellular growth inhibition.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9536 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Whole Cell, HELA Whole Cell, A549 Whole Cell, HEPG2 Whole Cell, 293T Whole Cell
Validation Images & Assay Conditions
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Western blot analysis of Cdk7 using anti-Cdk7 antibody (PB9536).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: MCF-7 Whole Cell Lysate at 40ug,
Lane 2: HELA Whole Cell Lysate at 40ug,
Lane 3: A549 Whole Cell Lysate at 40ug,
Lane 4: HEPG2 Whole Cell Lysate at 40ug,
Lane 5: 293T Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk7 antigen affinity purified polyclonal antibody (Catalog # PB9536) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk7 at approximately 39 kDa. The expected band size for Cdk7 is at 39 kDa.
Specific Publications For Anti-Cdk7 Antibody Picoband® (PB9536)
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1 Customer Q&As for Anti-Cdk7 Antibody Picoband®
Question
We are currently using anti-Cdk7 antibody PB9536 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
C. Rodriguez
Verified customer
Asked: 2018-03-12
Answer
The anti-Cdk7 antibody (PB9536) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-12
