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- Table of Contents
CEBPA antibody (A00386-1) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of CACO-2 cells using anti-CEBP Alpha/CEBPA antibody (A00386-1).
Overlay histogram showing CACO-2 cells stained with A00386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Alpha/CEBPA Antibody (A00386-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with A00386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Alpha/CEBPA Antibody (A00386-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CEBP Alpha/CEBPA Antibody Picoband®
CEBPA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
CEBPA antibody was positively detected by WB in:
human THP-1 whole cell, human U-937 whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CEBPA antibody was positively detected by ICC/IF in:
HepG2 cell
CEBPA antibody was positively detected by FC in:
CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-CEBP Alpha/CEBPA Antibody Picoband®
View all CEBPA Antibodies
View all CEBPA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CEBP Alpha/CEBPA recombinant protein (Position: Y138-Q221).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated Molecular Weight
37.6 kDa
Background of CEBPA
CEBPA, CCAAT/enhancer-binding protein alpha is a protein that in humans is encoded by the CEBPA gene. The CEBPA gene is intronless. Using human/hamster somatic cell hybrids containing restricted fragments of human chromosome 19, the CEBPA gene is mapped to chromosome 19q13.1, between the GPI and TGFB1 genes. The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis.
Cite This Product
Anti-CEBP Alpha/CEBPA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00386-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Rabbit
95%
High Homology
Cow
95%
High Homology
Sheep
95%
High Homology
Zebrafish
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CEBPA antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CEBPA antibody
Flow Cytometry (FC) protocol for CEBPA antibody
Publications
Application:
Species:
Extracellular vesicles derived from dental follicle stem cells regulate tooth eruption by inhibiting osteoclast differentiation
Published: 2024-12-20
Journal: Frontiers in Cell and Developmental Biology
Application: WB
Impact Factor: 4.60
Species: Mouse
PMID: 39834384
Screening of the Lipid-Lowering Probiotic Lactiplantibacillus Plantarum SDJ09 and its Anti-Obesity Mechanism
Published: 2024-08-02
Journal: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Application: WB
Impact Factor: 3.10
Species: Mouse
PMID: 39093349
An intronic enhancer of Cebpa regulates adipocyte differentiation and adipose tissue development via long-range loop formation
Published: 2023-10-31
Journal: CELL PROLIFERATION
Application: IF
Impact Factor: 8.50
Species: Mouse
PMID: 37905345
Quinacrine attenuates diet-induced obesity by inhibiting adipogenesis via activation of AMPK signaling
Published: 2022-11-30
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 5.20
Species: Mouse
PMID: 36460132
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway
Published: 2022-07-04
Journal: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 2.34
Species: Mouse
PMID: 35786714
β-Cypermethrin promotes the adipogenesis of 3T3-L1 cells via inducing autophagy and shaping an adipogenesis-friendly microenvironment
Published: 2020-07-08
Journal: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Application: WB
Impact Factor: 2.84
Species: Mouse
PMID: 32637997
β-Cypermethrin Alleviated the Inhibitory Effect of Medium from RAW 264.7 Cells on 3T3-L1 Cell Maturation into Adipocytes
Published: 2020-03-31
Journal: LIPIDS
Application: WB
Impact Factor: 1.92
Species: Mouse
PMID: 32236964
Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head
Published: 2020-02-01
Journal: BONE
Application: WB
Impact Factor: 4.15
Species: Human
PMID: 32018039
LncRNA expression profiling of BMSCs in osteonecrosis of the femoral head associated with increased adipogenic and decreased osteogenic differentiation
Published: 2018-06-14
Journal: Scientific Reports
Application: IHC
Impact Factor: 4.12
Species: Human
PMID: 29904151
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A00386-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 18, 2026