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Product Info Summary
| SKU: | A01245 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Albumin Antibody
SKU/Catalog Number
A01245
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Albumin Antibody (Catalog # A01245). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Albumin antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Albumin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01245)
Host
Chicken
Contents
Albumin Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgY
Immunogen
Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin. The immunogen is located within amino acids 340 - 390 of Albumin.
Reactive Species
A01245 is reactive to ALB in Human, Mouse, Rat
Observed Molecular Weight
69 kDa
Calculated molecular weight
69.4 kDa
Background of ALB
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. It functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The resulting product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. At least two isoforms of Albumin are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01245 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; IHC-P/IF: 10-20 μg/mL; IHC-P: 2-10 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in mouse samples; Immunohistochemistry in human, mouse, and rat samples; . All other applications and species not yet tested.
Validation Images & Assay Conditions
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Western Blot Validation in Human Liver
Loading: 10 μg of lysates per lane.
Antibodies: Albumin, A01245, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Rat Liver
Loading: 10 μg of lysates per lane.
Antibodies: Albumin, A01245, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse Liver
Loading: 10 μg of lysates per lane.
Antibodies: Albumin, A01245, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of Albumin in Human Liver
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-albumin antibody (A01245) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of Albumin in Mouse Liver
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody (A01245) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of Albumin in Rat Liver
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody (A01245) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of Albumin in Mouse Liver
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with A01245 at 20 ???????/mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Specific Publications For Anti-Albumin Antibody (A01245)
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