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Product Info Summary
| SKU: | M00386 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-CEBP alpha Rabbit Monoclonal Antibody
SKU/Catalog Number
M00386
BM4562 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CEBP alpha Rabbit Monoclonal Antibody catalog # M00386. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEBP alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00386)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FIE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CEBP alpha
Reactive Species
M00386 is reactive to CEBPA in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
37.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00386 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IP 1:50
FC 1:50
Positive Control
WB: U937 cell
Validation Images & Assay Conditions
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Western blot analysis of CEBP alpha expression in U937 cell lysate.
Specific Publications For Anti-CEBP alpha Rabbit Monoclonal Antibody (M00386)
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4 Customer Q&As for Anti-CEBP alpha Rabbit Monoclonal Antibody
Question
We are currently using anti-CEBP alpha Rabbit Monoclonal antibody M00386 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-CEBP alpha Rabbit Monoclonal antibody (M00386) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
We were content with the WB result of your anti-CEBP alpha Rabbit Monoclonal antibody. However we have observed positive staining in adipose tissue of abdominal region nucleolus using this antibody. Is that expected? Could you tell me where is CEBPA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
According to literature, adipose tissue of abdominal region does express CEBPA. Generally CEBPA expresses in nucleus., isoform 4: nucleus, nucleolus. Regarding which tissues have CEBPA expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15057824
Pancreas, Pubmed ID: 15489334
Umbilical cord, Pubmed ID: 7575576
Boster Scientific Support
Answered: 2017-08-07
Question
We have seen staining in human umbilical cord. Any tips? Is anti-CEBP alpha Rabbit Monoclonal antibody supposed to stain umbilical cord positively?
N. Patel
Verified customer
Asked: 2014-10-03
Answer
Based on literature umbilical cord does express CEBPA. Based on Uniprot.org, CEBPA is expressed in adipose tissue of abdominal region, umbilical cord, liver, pancreas, among other tissues. Regarding which tissues have CEBPA expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15057824
Pancreas, Pubmed ID: 15489334
Umbilical cord, Pubmed ID: 7575576
Boster Scientific Support
Answered: 2014-10-03
Question
Our lab used your anti-CEBP alpha Rabbit Monoclonal antibody for IP on umbilical cord in a previous experiment. I am using mouse, and We are going to use the antibody for WB next. We need examining umbilical cord as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for WB?
J. Patel
Verified customer
Asked: 2013-06-13
Answer
I took a look at the website and datasheets of our anti-CEBP alpha Rabbit Monoclonal antibody and it appears that M00386 has been validated on mouse in both IP and WB. Thus M00386 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-06-13
