Anti-Chk2/CHEK2 Antibody

Boster Bio Anti-Chk2/CHEK2 Antibody catalog # PA1202. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1202
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Chk2/CHEK2 Antibody

See all Chk2 products

SKU/Catalog Number

PA1202

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Chk2/CHEK2 Antibody catalog # PA1202. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Chk2/CHEK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1202)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Chk2 (427-441aa FSEHRTQVSLKDQIT), different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1202 is reactive to CHEK2 in Human, Mouse, Rat

Applications

PA1202 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For CHEK2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O96017

Gene ID

11200

Gene Name

CHEK2

Full Name

Serine/threonine-protein kinase Chk2

Weight

60.915kDa

Superfamily

protein kinase superfamily

Alternative Names

CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog CHEK2 CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1 checkpoint kinase 2 serine/threonine-protein kinase Chk2|CHK2 checkpoint homolog|cds1 homolog|checkpoint-like protein CHK2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CHEK2, check out the CHEK2 Infographic

CHEK2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CHEK2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PA1202

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4 Customer Q&As for Anti-Chk2/CHEK2 Antibody

Question

My boss were happy with the WB result of your anti-Chk2/CHEK2 antibody. However we have been able to see positive staining in t-cell isoform 12: nucleus. using this antibody. Is that expected? Could you tell me where is CHEK2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

From literature, t-cell does express CHEK2. Generally CHEK2 expresses in isoform 2: nucleus. note=isoform 10 is, isoform 4: nucleus., isoform 7: nucleus., isoform 9: nucleus., isoform 12: nucleus., nucleus, pml body. nucleus, nucleoplasm. Regarding which tissues have CHEK2 expression, here are a few articles citing expression in various tissues:
Mammary gland, Pubmed ID: 15361853
Muscle, Pubmed ID: 15489334
T-cell, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-04-10

Question

I am looking for using your anti-Chk2/CHEK2 antibody for regulation of tp53 activity through methylation studies. Has this antibody been tested with western blotting on human k562? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

Thank you for your inquiry. This PA1202 anti-Chk2/CHEK2 antibody is tested on human k562, hepg2 whole cell lysates, k562 whole cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-24

Question

We are currently using anti-Chk2/CHEK2 antibody PA1202 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

The anti-Chk2/CHEK2 antibody (PA1202) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-11

Question

We have been able to see staining in mouse t-cell. What should we do? Is anti-Chk2/CHEK2 antibody supposed to stain t-cell positively?

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

From literature t-cell does express CHEK2. From Uniprot.org, CHEK2 is expressed in tibial nerve, mammary gland, colon carcinoma, t-cell, muscle, among other tissues. Regarding which tissues have CHEK2 expression, here are a few articles citing expression in various tissues:
Mammary gland, Pubmed ID: 15361853
Muscle, Pubmed ID: 15489334
T-cell, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-07-10

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1202
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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