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- Table of Contents
CHEK2 antibody (A00277) performs well in ELISA, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 μg/mL
Red: Chk2 Antibody (A00277)
Blue: DAPI staining
Red: Chk2 Antibody (A00277)
Blue: DAPI staining
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Anti-Chk2 CHEK2 Antibody
CHEK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
Chk2 antibody can be used for detection of Chk2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Chk2 CHEK2 Antibody
View all CHEK2 Antibodies
View all CHEK2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Chk2 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
Chk2 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2. The immunogen is located within the first 50 amino acids of Chk2.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
60.9 kDa
Background of CHEK2
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
Cite This Product
Anti-Chk2 CHEK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00277)
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Product Promise
We promise Anti-Chk2 CHEK2 Antibody performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00277
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026