Anti-Chk2 CHEK2 Antibody

Product Name

Anti-Chk2 CHEK2 Antibody

View all CHEK2 Antibodies

SKU/Catalog Number

A00277

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Chk2 CHEK2 Antibody (Catalog # A00277). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Chk2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Chk2 CHEK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00277)

Host

Rabbit

Contents

Chk2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2. The immunogen is located within the first 50 amino acids of Chk2.

Reactive Species

A00277 is reactive to CHEK2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

60.9 kDa

Background of CHEK2

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00277 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Chk2 antibody can be used for detection of Chk2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CHEK2

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