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Product Info Summary
| SKU: | M00277-1 |
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| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IF, IHC, WB |
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Product info
Product Name
Anti-Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI5C4]
SKU/Catalog Number
M00277-1
Size
100 µl
Description
Boster Bio CHEK2 (CHK2) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4). Catalog# M00277-1. Tested in FC, IF, IHC, IP, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI5C4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00277-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5C4
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00277-1 is reactive to CHEK2 in Dog, Human, Mouse, Rat
Reconstitution
Calculated molecular weight
60.915kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00277-1 is guaranteed for Flow Cytometry, IP, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Flow cytometric Analysis of Jurkat cells
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Anti-CHEK2 mouse monoclonal antibody (M00277-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2.
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Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (M00277-1).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CHEK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and CHEK2-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-CHEK2 monoclonal antibody M00277-1. Then the blotted membrane was stripped and reprobed with anti-β-actin as a loading control (1:500).
Protein Target Info & Infographic
Gene/Protein Information For CHEK2 (Source: Uniprot.org, NCBI)
Gene Name
CHEK2
Full Name
Serine/threonine-protein kinase Chk2
Weight
60.915kDa
Superfamily
protein kinase superfamily
Alternative Names
CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog CHEK2 CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1 checkpoint kinase 2 serine/threonine-protein kinase Chk2|CHK2 checkpoint homolog|cds1 homolog|checkpoint-like protein CHK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHEK2, check out the CHEK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHEK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI5C4] (M00277-1)
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