Product Info Summary
| SKU: | M04360 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-CISD1 Mouse Monoclonal Antibody [Clone ID: OTI2B3]
SKU/Catalog Number
M04360
Size
100 µl
Description
Boster Bio CISD1 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3). Catalog# M04360. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-CISD1 Mouse Monoclonal Antibody [Clone ID: OTI2B3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04360)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2B3
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CISD1 (NP_060934) produced in HEK293T cell.
Reactive Species
M04360 is reactive to CISD1 in Human, Mouse, Rat
Calculated molecular weight
12.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04360 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100~400
WB: 1:200
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
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Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-CISD1 monoclonal antibody (1:200).
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Flow cytometric Analysis of Jurkat cells
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Anti-CISD1 mouse monoclonal antibody (M04360) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1.
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Immunofluorescent staining of HeLa cells using anti-CISD1 mouse monoclonal antibody (M04360).
Specific Publications For Anti-CISD1 Mouse Monoclonal Antibody [Clone ID: OTI2B3] (M04360)
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